Experiment: Varel_Bryant_medium_Glucose with Trimethoprim 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0205 and BT0206 are separated by 657 nucleotides BT0206 and BT0207 are separated by 18 nucleotides
BT0205: BT0205 - glutamine-dependent NAD+ synthetase (NCBI ptt file), at 220,121 to 222,046
BT0205
BT0206: BT0206 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 222,704 to 225,646
BT0206
BT0207: BT0207 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 225,665 to 227,200
BT0207
Position (kb) 222
223
224
225
226 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 222.060 kb on - strand at 222.066 kb on - strand at 222.139 kb on - strand at 222.228 kb on - strand at 222.400 kb on - strand at 222.400 kb on - strand at 222.564 kb on - strand at 222.602 kb on - strand at 222.602 kb on - strand at 222.602 kb on - strand at 222.602 kb on - strand at 222.783 kb on - strand at 222.867 kb on + strand at 222.886 kb on + strand at 223.021 kb on - strand, within BT0206 at 223.039 kb on - strand, within BT0206 at 223.039 kb on - strand, within BT0206 at 223.039 kb on - strand, within BT0206 at 223.039 kb on - strand, within BT0206 at 223.093 kb on + strand, within BT0206 at 223.232 kb on - strand, within BT0206 at 223.277 kb on - strand, within BT0206 at 223.317 kb on + strand, within BT0206 at 223.358 kb on - strand, within BT0206 at 223.403 kb on - strand, within BT0206 at 223.457 kb on - strand, within BT0206 at 223.554 kb on - strand, within BT0206 at 223.561 kb on - strand, within BT0206 at 223.667 kb on + strand, within BT0206 at 223.849 kb on + strand, within BT0206 at 223.891 kb on + strand, within BT0206 at 224.009 kb on - strand, within BT0206 at 224.009 kb on - strand, within BT0206 at 224.125 kb on + strand, within BT0206 at 224.126 kb on - strand, within BT0206 at 224.250 kb on + strand, within BT0206 at 224.282 kb on - strand, within BT0206 at 224.353 kb on + strand, within BT0206 at 224.356 kb on + strand, within BT0206 at 224.357 kb on - strand, within BT0206 at 224.357 kb on - strand, within BT0206 at 224.357 kb on - strand, within BT0206 at 224.539 kb on - strand, within BT0206 at 224.539 kb on - strand, within BT0206 at 224.663 kb on + strand, within BT0206 at 224.664 kb on - strand, within BT0206 at 224.789 kb on - strand, within BT0206 at 224.789 kb on - strand, within BT0206 at 224.789 kb on - strand, within BT0206 at 224.789 kb on - strand, within BT0206 at 224.792 kb on - strand, within BT0206 at 224.803 kb on - strand, within BT0206 at 224.832 kb on + strand, within BT0206 at 224.860 kb on + strand, within BT0206 at 224.913 kb on - strand, within BT0206 at 224.986 kb on - strand, within BT0206 at 224.993 kb on + strand, within BT0206 at 224.994 kb on - strand, within BT0206 at 224.997 kb on + strand, within BT0206 at 225.205 kb on + strand, within BT0206 at 225.300 kb on + strand, within BT0206 at 225.303 kb on - strand, within BT0206 at 225.303 kb on - strand, within BT0206 at 225.312 kb on - strand, within BT0206 at 225.554 kb on - strand at 225.588 kb on - strand at 225.651 kb on - strand at 225.688 kb on + strand at 225.780 kb on + strand at 225.780 kb on + strand at 225.781 kb on - strand at 225.781 kb on - strand at 225.859 kb on - strand, within BT0207 at 225.953 kb on - strand, within BT0207 at 225.956 kb on - strand, within BT0207 at 226.192 kb on - strand, within BT0207 at 226.251 kb on - strand, within BT0207 at 226.335 kb on + strand, within BT0207 at 226.336 kb on - strand, within BT0207 at 226.352 kb on + strand, within BT0207 at 226.353 kb on - strand, within BT0207 at 226.365 kb on - strand, within BT0207 at 226.403 kb on - strand, within BT0207 at 226.423 kb on + strand, within BT0207 at 226.430 kb on + strand, within BT0207 at 226.458 kb on - strand, within BT0207 at 226.506 kb on - strand, within BT0207 at 226.511 kb on - strand, within BT0207 at 226.514 kb on + strand, within BT0207 at 226.611 kb on - strand, within BT0207 at 226.611 kb on - strand, within BT0207
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with Trimethoprim 0.025 mM remove 222,060 - -2.2 222,066 - -3.9 222,139 - -2.3 222,228 - -1.1 222,400 - -2.6 222,400 - -1.3 222,564 - -2.1 222,602 - -1.1 222,602 - -3.0 222,602 - +1.3 222,602 - +1.5 222,783 - -0.7 222,867 + -0.1 222,886 + -0.1 223,021 - BT0206 0.11 -2.1 223,039 - BT0206 0.11 -5.2 223,039 - BT0206 0.11 -4.2 223,039 - BT0206 0.11 +0.4 223,039 - BT0206 0.11 -1.6 223,093 + BT0206 0.13 +0.9 223,232 - BT0206 0.18 -1.6 223,277 - BT0206 0.19 -0.4 223,317 + BT0206 0.21 -3.1 223,358 - BT0206 0.22 -1.1 223,403 - BT0206 0.24 +1.9 223,457 - BT0206 0.26 -2.1 223,554 - BT0206 0.29 -1.6 223,561 - BT0206 0.29 -1.4 223,667 + BT0206 0.33 -0.8 223,849 + BT0206 0.39 +0.9 223,891 + BT0206 0.40 -0.3 224,009 - BT0206 0.44 -1.6 224,009 - BT0206 0.44 -0.1 224,125 + BT0206 0.48 -0.1 224,126 - BT0206 0.48 -1.2 224,250 + BT0206 0.53 -0.3 224,282 - BT0206 0.54 -1.1 224,353 + BT0206 0.56 -1.6 224,356 + BT0206 0.56 -2.1 224,357 - BT0206 0.56 -2.6 224,357 - BT0206 0.56 -1.7 224,357 - BT0206 0.56 -1.5 224,539 - BT0206 0.62 -2.9 224,539 - BT0206 0.62 -0.9 224,663 + BT0206 0.67 -0.1 224,664 - BT0206 0.67 -2.6 224,789 - BT0206 0.71 -2.1 224,789 - BT0206 0.71 -1.9 224,789 - BT0206 0.71 +0.3 224,789 - BT0206 0.71 -1.3 224,792 - BT0206 0.71 -1.8 224,803 - BT0206 0.71 -3.1 224,832 + BT0206 0.72 -1.6 224,860 + BT0206 0.73 -0.8 224,913 - BT0206 0.75 -0.1 224,986 - BT0206 0.78 -2.2 224,993 + BT0206 0.78 -1.6 224,994 - BT0206 0.78 -3.2 224,997 + BT0206 0.78 +1.3 225,205 + BT0206 0.85 -1.6 225,300 + BT0206 0.88 -1.1 225,303 - BT0206 0.88 -1.6 225,303 - BT0206 0.88 -1.1 225,312 - BT0206 0.89 -1.5 225,554 - -2.7 225,588 - -3.9 225,651 - -2.2 225,688 + +1.7 225,780 + -1.5 225,780 + -0.1 225,781 - -2.2 225,781 - +0.5 225,859 - BT0207 0.13 -2.2 225,953 - BT0207 0.19 -2.1 225,956 - BT0207 0.19 -2.6 226,192 - BT0207 0.34 -0.1 226,251 - BT0207 0.38 -1.6 226,335 + BT0207 0.44 -2.4 226,336 - BT0207 0.44 -2.1 226,352 + BT0207 0.45 -2.1 226,353 - BT0207 0.45 -1.1 226,365 - BT0207 0.46 -0.1 226,403 - BT0207 0.48 -2.1 226,423 + BT0207 0.49 +2.7 226,430 + BT0207 0.50 -2.6 226,458 - BT0207 0.52 +2.5 226,506 - BT0207 0.55 -1.1 226,511 - BT0207 0.55 -1.1 226,514 + BT0207 0.55 -0.1 226,611 - BT0207 0.62 +1.5 226,611 - BT0207 0.62 +2.1
Or see this region's nucleotide sequence