Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4758 and Psyr_4759 are separated by 98 nucleotides Psyr_4759 and Psyr_4760 are separated by 9 nucleotides Psyr_4760 and Psyr_4761 are separated by 97 nucleotides Psyr_4761 and Psyr_4762 are separated by 45 nucleotides
Psyr_4758: Psyr_4758 - Phosphopantetheine adenylyltransferase, at 5,634,887 to 5,635,366
_4758
Psyr_4759: Psyr_4759 - 4Fe-4S ferredoxin, iron-sulfur binding protein, at 5,635,465 to 5,635,716
_4759
Psyr_4760: Psyr_4760 - conserved hypothetical protein, at 5,635,726 to 5,636,064
_4760
Psyr_4761: Psyr_4761 - DNA-(apurinic or apyrimidinic site) lyase / Formamidopyrimidine-DNA glycosylase, at 5,636,162 to 5,636,974
_4761
Psyr_4762: Psyr_4762 - conserved hypothetical protein, at 5,637,020 to 5,637,886
_4762
Position (kb)
5635
5636
5637 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5634.744 kb on + strand at 5634.796 kb on - strand at 5635.365 kb on - strand at 5635.428 kb on + strand at 5635.727 kb on + strand at 5635.728 kb on - strand at 5635.958 kb on + strand, within Psyr_4760 at 5636.090 kb on + strand at 5636.090 kb on + strand at 5636.090 kb on + strand at 5636.090 kb on + strand at 5636.090 kb on + strand at 5636.090 kb on + strand at 5636.090 kb on + strand at 5636.090 kb on + strand at 5636.091 kb on - strand at 5636.091 kb on - strand at 5636.091 kb on - strand at 5636.091 kb on - strand at 5636.091 kb on - strand at 5636.094 kb on + strand at 5636.094 kb on + strand at 5636.094 kb on + strand at 5636.095 kb on - strand at 5636.108 kb on + strand at 5636.108 kb on + strand at 5636.108 kb on + strand at 5636.108 kb on + strand at 5636.109 kb on - strand at 5636.109 kb on - strand at 5636.109 kb on - strand at 5636.110 kb on + strand at 5636.110 kb on + strand at 5636.110 kb on + strand at 5636.110 kb on + strand at 5636.111 kb on - strand at 5636.111 kb on - strand at 5636.111 kb on - strand at 5636.111 kb on - strand at 5636.111 kb on - strand at 5636.111 kb on - strand at 5636.128 kb on - strand at 5636.158 kb on + strand at 5636.158 kb on + strand at 5636.158 kb on + strand at 5636.158 kb on + strand at 5636.158 kb on + strand at 5636.159 kb on - strand at 5636.159 kb on - strand at 5636.159 kb on - strand at 5636.159 kb on - strand at 5636.184 kb on + strand at 5636.185 kb on - strand at 5636.185 kb on - strand at 5636.460 kb on + strand, within Psyr_4761 at 5636.460 kb on + strand, within Psyr_4761 at 5636.460 kb on + strand, within Psyr_4761 at 5636.460 kb on + strand, within Psyr_4761 at 5636.460 kb on + strand, within Psyr_4761 at 5636.460 kb on + strand, within Psyr_4761 at 5636.461 kb on - strand, within Psyr_4761 at 5636.461 kb on - strand, within Psyr_4761 at 5636.461 kb on - strand, within Psyr_4761 at 5636.461 kb on - strand, within Psyr_4761 at 5636.461 kb on - strand, within Psyr_4761 at 5636.461 kb on - strand, within Psyr_4761 at 5636.461 kb on - strand, within Psyr_4761 at 5636.464 kb on + strand, within Psyr_4761 at 5636.464 kb on + strand, within Psyr_4761 at 5636.464 kb on + strand, within Psyr_4761 at 5636.464 kb on + strand, within Psyr_4761 at 5636.465 kb on - strand, within Psyr_4761 at 5636.541 kb on + strand, within Psyr_4761 at 5636.659 kb on - strand, within Psyr_4761 at 5636.799 kb on + strand, within Psyr_4761 at 5636.799 kb on + strand, within Psyr_4761 at 5636.800 kb on - strand, within Psyr_4761 at 5636.800 kb on - strand, within Psyr_4761 at 5636.800 kb on - strand, within Psyr_4761 at 5636.800 kb on - strand, within Psyr_4761 at 5636.964 kb on - strand at 5636.964 kb on - strand at 5637.057 kb on + strand at 5637.057 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 5,634,744 + +0.3 5,634,796 - -0.8 5,635,365 - -0.3 5,635,428 + -0.5 5,635,727 + +0.3 5,635,728 - -0.3 5,635,958 + Psyr_4760 0.68 -1.1 5,636,090 + +1.0 5,636,090 + +0.3 5,636,090 + -1.6 5,636,090 + +0.4 5,636,090 + +1.4 5,636,090 + -1.1 5,636,090 + +0.9 5,636,090 + -0.1 5,636,091 - -0.3 5,636,091 - -1.1 5,636,091 - +0.6 5,636,091 - +0.7 5,636,091 - +1.2 5,636,094 + +0.6 5,636,094 + +0.2 5,636,094 + -0.5 5,636,095 - -1.8 5,636,108 + -0.0 5,636,108 + +0.2 5,636,108 + +0.5 5,636,108 + +0.0 5,636,109 - -0.0 5,636,109 - -0.5 5,636,109 - +0.4 5,636,110 + +0.5 5,636,110 + -2.8 5,636,110 + -0.4 5,636,110 + -1.4 5,636,111 - +1.5 5,636,111 - +0.3 5,636,111 - -1.0 5,636,111 - +0.8 5,636,111 - -1.3 5,636,111 - +1.5 5,636,128 - +0.5 5,636,158 + -0.5 5,636,158 + +0.4 5,636,158 + +0.9 5,636,158 + +0.3 5,636,158 + +1.2 5,636,159 - +1.1 5,636,159 - -0.2 5,636,159 - -0.6 5,636,159 - -0.1 5,636,184 + +1.0 5,636,185 - -0.2 5,636,185 - +0.2 5,636,460 + Psyr_4761 0.37 -0.1 5,636,460 + Psyr_4761 0.37 -0.5 5,636,460 + Psyr_4761 0.37 -1.8 5,636,460 + Psyr_4761 0.37 +0.5 5,636,460 + Psyr_4761 0.37 -0.7 5,636,460 + Psyr_4761 0.37 +1.2 5,636,461 - Psyr_4761 0.37 -0.0 5,636,461 - Psyr_4761 0.37 +0.6 5,636,461 - Psyr_4761 0.37 -0.1 5,636,461 - Psyr_4761 0.37 +0.1 5,636,461 - Psyr_4761 0.37 +0.0 5,636,461 - Psyr_4761 0.37 +0.5 5,636,461 - Psyr_4761 0.37 +0.5 5,636,464 + Psyr_4761 0.37 +0.3 5,636,464 + Psyr_4761 0.37 +0.5 5,636,464 + Psyr_4761 0.37 +0.7 5,636,464 + Psyr_4761 0.37 -0.6 5,636,465 - Psyr_4761 0.37 +0.6 5,636,541 + Psyr_4761 0.47 -0.0 5,636,659 - Psyr_4761 0.61 -0.2 5,636,799 + Psyr_4761 0.78 +0.9 5,636,799 + Psyr_4761 0.78 +0.4 5,636,800 - Psyr_4761 0.78 +0.0 5,636,800 - Psyr_4761 0.78 -1.3 5,636,800 - Psyr_4761 0.78 -0.7 5,636,800 - Psyr_4761 0.78 +0.3 5,636,964 - -0.4 5,636,964 - +0.3 5,637,057 + +1.4 5,637,057 + -1.1
Or see this region's nucleotide sequence