Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4634 and Psyr_4635 are separated by 98 nucleotides Psyr_4635 and Psyr_4636 overlap by 10 nucleotides Psyr_4636 and Psyr_4637 are separated by 65 nucleotides
Psyr_4634: Psyr_4634 - Polynucleotide adenylyltransferase:Metal-dependent phosphohydrolase, HD subdomain protein, at 5,495,417 to 5,496,643
_4634
Psyr_4635: Psyr_4635 - 7, 8-Dihydro-6-hydroxymethylpterin-pyrophosphokinase, HPPK, at 5,496,742 to 5,497,266
_4635
Psyr_4636: Psyr_4636 - Dihydroneopterin aldolase family:Dihydroneopterin aldolase, at 5,497,257 to 5,497,610
_4636
Psyr_4637: Psyr_4637 - acyl-phosphate glycerol-3-phosphate acyltransferase, at 5,497,676 to 5,498,254
_4637
Position (kb) 5496
5497
5498 Strain fitness (log2 ratio) -1
0
1
2 at 5496.093 kb on + strand, within Psyr_4634 at 5496.105 kb on + strand, within Psyr_4634 at 5496.623 kb on + strand at 5496.624 kb on - strand at 5496.692 kb on + strand at 5496.692 kb on + strand at 5496.692 kb on + strand at 5496.692 kb on + strand at 5496.693 kb on - strand at 5496.747 kb on + strand at 5496.747 kb on + strand at 5496.748 kb on - strand at 5496.904 kb on + strand, within Psyr_4635 at 5497.651 kb on + strand at 5497.971 kb on - strand, within Psyr_4637 at 5497.971 kb on - strand, within Psyr_4637 at 5497.971 kb on - strand, within Psyr_4637 at 5497.971 kb on - strand, within Psyr_4637 at 5497.971 kb on - strand, within Psyr_4637 at 5498.078 kb on + strand, within Psyr_4637 at 5498.078 kb on + strand, within Psyr_4637 at 5498.079 kb on - strand, within Psyr_4637 at 5498.079 kb on - strand, within Psyr_4637 at 5498.218 kb on - strand at 5498.218 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 5,496,093 + Psyr_4634 0.55 +0.2 5,496,105 + Psyr_4634 0.56 -1.3 5,496,623 + +1.1 5,496,624 - +0.1 5,496,692 + +0.5 5,496,692 + -0.1 5,496,692 + -0.1 5,496,692 + -1.0 5,496,693 - +0.5 5,496,747 + +0.7 5,496,747 + +0.5 5,496,748 - +1.5 5,496,904 + Psyr_4635 0.31 -1.5 5,497,651 + -1.2 5,497,971 - Psyr_4637 0.51 -0.5 5,497,971 - Psyr_4637 0.51 +0.2 5,497,971 - Psyr_4637 0.51 +2.0 5,497,971 - Psyr_4637 0.51 +0.4 5,497,971 - Psyr_4637 0.51 -1.2 5,498,078 + Psyr_4637 0.69 -0.2 5,498,078 + Psyr_4637 0.69 +0.0 5,498,079 - Psyr_4637 0.70 -0.5 5,498,079 - Psyr_4637 0.70 -0.1 5,498,218 - -0.2 5,498,218 - +0.3
Or see this region's nucleotide sequence