Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_RNA74 and Psyr_4565 are separated by 507 nucleotides Psyr_4565 and Psyr_4566 are separated by 201 nucleotides
Psyr_RNA74: Psyr_RNA74 - 16S ribosomal RNA, at 5,426,727 to 5,428,264
_RNA74
Psyr_4565: Psyr_4565 - tyrosyl-tRNA synthetase, at 5,428,772 to 5,429,983
_4565
Psyr_4566: Psyr_4566 - Peptidase M23B, at 5,430,185 to 5,431,612
_4566
Position (kb)
5428
5429
5430 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5428.743 kb on + strand at 5428.743 kb on + strand at 5428.745 kb on + strand at 5428.745 kb on + strand at 5430.015 kb on - strand at 5430.111 kb on - strand at 5430.115 kb on + strand at 5430.181 kb on - strand at 5430.184 kb on + strand at 5430.184 kb on + strand at 5430.185 kb on - strand at 5430.355 kb on - strand, within Psyr_4566 at 5430.355 kb on - strand, within Psyr_4566 at 5430.597 kb on + strand, within Psyr_4566 at 5430.598 kb on - strand, within Psyr_4566 at 5430.598 kb on - strand, within Psyr_4566 at 5430.752 kb on + strand, within Psyr_4566 at 5430.797 kb on + strand, within Psyr_4566 at 5430.801 kb on + strand, within Psyr_4566 at 5430.801 kb on + strand, within Psyr_4566 at 5430.801 kb on + strand, within Psyr_4566 at 5430.801 kb on + strand, within Psyr_4566 at 5430.801 kb on + strand, within Psyr_4566 at 5430.802 kb on - strand, within Psyr_4566 at 5430.802 kb on - strand, within Psyr_4566 at 5430.802 kb on - strand, within Psyr_4566 at 5430.802 kb on - strand, within Psyr_4566 at 5430.802 kb on - strand, within Psyr_4566 at 5430.802 kb on - strand, within Psyr_4566 at 5430.802 kb on - strand, within Psyr_4566 at 5430.859 kb on + strand, within Psyr_4566 at 5430.859 kb on + strand, within Psyr_4566 at 5430.859 kb on + strand, within Psyr_4566 at 5430.859 kb on + strand, within Psyr_4566 at 5430.859 kb on + strand, within Psyr_4566 at 5430.860 kb on - strand, within Psyr_4566 at 5430.860 kb on - strand, within Psyr_4566 at 5430.860 kb on - strand, within Psyr_4566 at 5430.860 kb on - strand, within Psyr_4566 at 5430.945 kb on + strand, within Psyr_4566 at 5430.945 kb on + strand, within Psyr_4566 at 5430.945 kb on + strand, within Psyr_4566 at 5430.945 kb on + strand, within Psyr_4566 at 5430.946 kb on - strand, within Psyr_4566 at 5430.947 kb on + strand, within Psyr_4566 at 5430.948 kb on - strand, within Psyr_4566 at 5430.948 kb on - strand, within Psyr_4566 at 5430.948 kb on - strand, within Psyr_4566 at 5430.948 kb on - strand, within Psyr_4566 at 5430.948 kb on - strand, within Psyr_4566 at 5430.948 kb on - strand, within Psyr_4566 at 5430.979 kb on - strand, within Psyr_4566 at 5430.979 kb on - strand, within Psyr_4566
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 5,428,743 + -2.6 5,428,743 + -1.9 5,428,745 + +2.4 5,428,745 + -1.6 5,430,015 - +2.7 5,430,111 - -1.7 5,430,115 + +0.6 5,430,181 - +0.1 5,430,184 + -0.1 5,430,184 + -1.1 5,430,185 - -1.1 5,430,355 - Psyr_4566 0.12 -0.9 5,430,355 - Psyr_4566 0.12 +0.4 5,430,597 + Psyr_4566 0.29 -0.1 5,430,598 - Psyr_4566 0.29 +0.4 5,430,598 - Psyr_4566 0.29 +0.4 5,430,752 + Psyr_4566 0.40 +1.4 5,430,797 + Psyr_4566 0.43 +0.4 5,430,801 + Psyr_4566 0.43 -0.9 5,430,801 + Psyr_4566 0.43 -0.9 5,430,801 + Psyr_4566 0.43 -0.4 5,430,801 + Psyr_4566 0.43 -0.1 5,430,801 + Psyr_4566 0.43 -1.9 5,430,802 - Psyr_4566 0.43 -0.6 5,430,802 - Psyr_4566 0.43 +2.0 5,430,802 - Psyr_4566 0.43 +1.4 5,430,802 - Psyr_4566 0.43 -2.5 5,430,802 - Psyr_4566 0.43 -1.1 5,430,802 - Psyr_4566 0.43 +1.4 5,430,802 - Psyr_4566 0.43 -1.1 5,430,859 + Psyr_4566 0.47 -1.1 5,430,859 + Psyr_4566 0.47 -2.6 5,430,859 + Psyr_4566 0.47 -0.6 5,430,859 + Psyr_4566 0.47 -0.1 5,430,859 + Psyr_4566 0.47 -0.9 5,430,860 - Psyr_4566 0.47 -0.6 5,430,860 - Psyr_4566 0.47 -1.1 5,430,860 - Psyr_4566 0.47 -0.1 5,430,860 - Psyr_4566 0.47 +0.4 5,430,945 + Psyr_4566 0.53 -0.6 5,430,945 + Psyr_4566 0.53 -2.6 5,430,945 + Psyr_4566 0.53 +0.4 5,430,945 + Psyr_4566 0.53 -1.4 5,430,946 - Psyr_4566 0.53 -1.1 5,430,947 + Psyr_4566 0.53 +1.4 5,430,948 - Psyr_4566 0.53 +0.4 5,430,948 - Psyr_4566 0.53 -0.1 5,430,948 - Psyr_4566 0.53 +1.4 5,430,948 - Psyr_4566 0.53 -0.1 5,430,948 - Psyr_4566 0.53 -0.1 5,430,948 - Psyr_4566 0.53 -1.1 5,430,979 - Psyr_4566 0.56 -1.6 5,430,979 - Psyr_4566 0.56 +1.4
Or see this region's nucleotide sequence