Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4455 and Psyr_4456 overlap by 4 nucleotides Psyr_4456 and Psyr_4457 are separated by 85 nucleotides Psyr_4457 and Psyr_4458 are separated by 16 nucleotides Psyr_4458 and Psyr_4459 overlap by 4 nucleotides
Psyr_4455: Psyr_4455 - conserved hypothetical protein, at 5,319,696 to 5,320,115
_4455
Psyr_4456: Psyr_4456 - GTP cyclohydrolase II, at 5,320,112 to 5,320,777
_4456
Psyr_4457: Psyr_4457 - thiamine-phosphate kinase, at 5,320,863 to 5,321,828
_4457
Psyr_4458: Psyr_4458 - Antitermination protein NusB, at 5,321,845 to 5,322,342
_4458
Psyr_4459: Psyr_4459 - 6,7-dimethyl-8-ribityllumazine synthase, at 5,322,339 to 5,322,815
_4459
Position (kb)
5320
5321
5322 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5319.926 kb on - strand, within Psyr_4455 at 5319.963 kb on + strand, within Psyr_4455 at 5319.963 kb on + strand, within Psyr_4455 at 5319.963 kb on + strand, within Psyr_4455 at 5320.026 kb on + strand, within Psyr_4455 at 5320.748 kb on - strand at 5320.781 kb on - strand at 5320.781 kb on - strand at 5320.781 kb on - strand at 5320.781 kb on - strand at 5320.826 kb on - strand at 5320.826 kb on - strand at 5320.826 kb on - strand at 5320.826 kb on - strand at 5320.826 kb on - strand at 5320.847 kb on - strand at 5320.847 kb on - strand at 5320.847 kb on - strand at 5320.847 kb on - strand at 5320.847 kb on - strand at 5320.847 kb on - strand at 5320.847 kb on - strand at 5320.847 kb on - strand at 5320.847 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 5,319,926 - Psyr_4455 0.55 -0.4 5,319,963 + Psyr_4455 0.64 -0.6 5,319,963 + Psyr_4455 0.64 -0.1 5,319,963 + Psyr_4455 0.64 -0.4 5,320,026 + Psyr_4455 0.79 +1.2 5,320,748 - -1.8 5,320,781 - +2.0 5,320,781 - +0.4 5,320,781 - +0.4 5,320,781 - -0.4 5,320,826 - +0.2 5,320,826 - -1.4 5,320,826 - +1.2 5,320,826 - +1.2 5,320,826 - -3.1 5,320,847 - +0.4 5,320,847 - +0.8 5,320,847 - -0.3 5,320,847 - +0.6 5,320,847 - +0.6 5,320,847 - -0.1 5,320,847 - +1.2 5,320,847 - +0.3 5,320,847 - +0.4
Or see this region's nucleotide sequence