Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4352 and Psyr_4353 are separated by 89 nucleotides Psyr_4353 and Psyr_4354 are separated by 41 nucleotides Psyr_4354 and Psyr_4355 are separated by 5 nucleotides Psyr_4355 and Psyr_4356 are separated by 147 nucleotides Psyr_4356 and Psyr_4357 are separated by 83 nucleotides
Psyr_4352: Psyr_4352 - leucyl-tRNA synthetase, at 5,205,329 to 5,207,935
_4352
Psyr_4353: Psyr_4353 - Rare lipoprotein B, at 5,208,025 to 5,208,630
_4353
Psyr_4354: Psyr_4354 - DNA polymerase III, delta subunit, at 5,208,672 to 5,209,709
_4354
Psyr_4355: Psyr_4355 - hypothetical protein, at 5,209,715 to 5,209,927
_4355
Psyr_4356: Psyr_4356 - conserved hypothetical protein, at 5,210,075 to 5,210,230
_4356
Psyr_4357: Psyr_4357 - HopAJ2 protein, at 5,210,314 to 5,211,651
_4357
Position (kb) 5208
5209
5210 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5207.973 kb on + strand at 5207.973 kb on + strand at 5207.973 kb on + strand at 5207.973 kb on + strand at 5207.973 kb on + strand at 5207.973 kb on + strand at 5207.973 kb on + strand at 5208.628 kb on + strand at 5208.628 kb on + strand at 5208.628 kb on + strand at 5208.628 kb on + strand at 5208.628 kb on + strand at 5208.628 kb on + strand at 5208.628 kb on + strand at 5209.708 kb on - strand at 5209.725 kb on + strand at 5209.725 kb on + strand at 5209.725 kb on + strand at 5209.725 kb on + strand at 5209.725 kb on + strand at 5209.725 kb on + strand at 5209.725 kb on + strand at 5209.726 kb on - strand at 5209.726 kb on - strand at 5209.726 kb on - strand at 5209.726 kb on - strand at 5209.726 kb on - strand at 5209.726 kb on - strand at 5209.726 kb on - strand at 5209.726 kb on - strand at 5209.726 kb on - strand at 5209.729 kb on + strand at 5209.729 kb on + strand at 5209.729 kb on + strand at 5209.729 kb on + strand at 5209.730 kb on - strand at 5209.730 kb on - strand at 5209.730 kb on - strand at 5209.730 kb on - strand at 5209.811 kb on + strand, within Psyr_4355 at 5209.811 kb on + strand, within Psyr_4355 at 5209.811 kb on + strand, within Psyr_4355 at 5209.812 kb on - strand, within Psyr_4355 at 5209.812 kb on - strand, within Psyr_4355 at 5209.812 kb on - strand, within Psyr_4355 at 5209.818 kb on + strand, within Psyr_4355 at 5209.819 kb on - strand, within Psyr_4355 at 5209.953 kb on + strand at 5209.954 kb on - strand at 5209.954 kb on - strand at 5209.954 kb on - strand at 5209.979 kb on + strand at 5209.979 kb on + strand at 5209.979 kb on + strand at 5209.979 kb on + strand at 5209.979 kb on + strand at 5209.979 kb on + strand at 5209.979 kb on + strand at 5209.980 kb on - strand at 5209.980 kb on - strand at 5209.980 kb on - strand at 5209.983 kb on + strand at 5209.983 kb on + strand at 5209.984 kb on - strand at 5209.984 kb on - strand at 5209.984 kb on - strand at 5209.995 kb on + strand at 5209.995 kb on + strand at 5209.996 kb on - strand at 5209.996 kb on - strand at 5210.006 kb on + strand at 5210.049 kb on + strand at 5210.049 kb on + strand at 5210.049 kb on + strand at 5210.050 kb on - strand at 5210.050 kb on - strand at 5210.050 kb on - strand at 5210.050 kb on - strand at 5210.194 kb on + strand, within Psyr_4356 at 5210.194 kb on + strand, within Psyr_4356 at 5210.195 kb on - strand, within Psyr_4356 at 5210.218 kb on + strand at 5210.219 kb on - strand at 5210.289 kb on - strand at 5210.297 kb on + strand at 5210.297 kb on + strand at 5210.582 kb on + strand, within Psyr_4357 at 5210.582 kb on + strand, within Psyr_4357 at 5210.583 kb on - strand, within Psyr_4357 at 5210.583 kb on - strand, within Psyr_4357 at 5210.675 kb on - strand, within Psyr_4357 at 5210.675 kb on - strand, within Psyr_4357
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 5,207,973 + +0.2 5,207,973 + +0.8 5,207,973 + -0.1 5,207,973 + +1.0 5,207,973 + +0.3 5,207,973 + +1.0 5,207,973 + -0.4 5,208,628 + -0.8 5,208,628 + +0.1 5,208,628 + -0.9 5,208,628 + -0.2 5,208,628 + -0.5 5,208,628 + -0.8 5,208,628 + +0.4 5,209,708 - -0.3 5,209,725 + -0.4 5,209,725 + +0.4 5,209,725 + -0.1 5,209,725 + -1.6 5,209,725 + -0.6 5,209,725 + +1.4 5,209,725 + -0.6 5,209,726 - +0.2 5,209,726 - +0.2 5,209,726 - -0.7 5,209,726 - +0.4 5,209,726 - -0.7 5,209,726 - +0.4 5,209,726 - -0.2 5,209,726 - +0.4 5,209,726 - -0.4 5,209,729 + +0.2 5,209,729 + -0.9 5,209,729 + -0.6 5,209,729 + +2.0 5,209,730 - -0.7 5,209,730 - +0.7 5,209,730 - -0.8 5,209,730 - -0.6 5,209,811 + Psyr_4355 0.45 -1.1 5,209,811 + Psyr_4355 0.45 -0.9 5,209,811 + Psyr_4355 0.45 -0.7 5,209,812 - Psyr_4355 0.46 +0.3 5,209,812 - Psyr_4355 0.46 -0.1 5,209,812 - Psyr_4355 0.46 +0.4 5,209,818 + Psyr_4355 0.48 +1.0 5,209,819 - Psyr_4355 0.49 -0.1 5,209,953 + +0.1 5,209,954 - -0.7 5,209,954 - -0.4 5,209,954 - +0.1 5,209,979 + -0.6 5,209,979 + -1.6 5,209,979 + -0.2 5,209,979 + +0.6 5,209,979 + -0.9 5,209,979 + +0.8 5,209,979 + +2.4 5,209,980 - -1.2 5,209,980 - +0.4 5,209,980 - -0.1 5,209,983 + +0.1 5,209,983 + +0.2 5,209,984 - -0.4 5,209,984 - +1.8 5,209,984 - -0.7 5,209,995 + -2.4 5,209,995 + +0.0 5,209,996 - +0.3 5,209,996 - +0.6 5,210,006 + +0.2 5,210,049 + -0.1 5,210,049 + -2.0 5,210,049 + -1.0 5,210,050 - +0.2 5,210,050 - -0.8 5,210,050 - -1.0 5,210,050 - -0.1 5,210,194 + Psyr_4356 0.76 +2.0 5,210,194 + Psyr_4356 0.76 +0.4 5,210,195 - Psyr_4356 0.77 +0.3 5,210,218 + -0.6 5,210,219 - -0.3 5,210,289 - +0.7 5,210,297 + -0.4 5,210,297 + -0.2 5,210,582 + Psyr_4357 0.20 -0.9 5,210,582 + Psyr_4357 0.20 +0.3 5,210,583 - Psyr_4357 0.20 +0.2 5,210,583 - Psyr_4357 0.20 -0.5 5,210,675 - Psyr_4357 0.27 +0.3 5,210,675 - Psyr_4357 0.27 -0.5
Or see this region's nucleotide sequence