Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4304 and Psyr_4305 are separated by 184 nucleotides Psyr_4305 and Psyr_4306 are separated by 104 nucleotides Psyr_4306 and Psyr_4307 are separated by 567 nucleotides
Psyr_4304: Psyr_4304 - Endonuclease/exonuclease/phosphatase, at 5,129,769 to 5,130,911
_4304
Psyr_4305: Psyr_4305 - conserved hypothetical protein, at 5,131,096 to 5,131,344
_4305
Psyr_4306: Psyr_4306 - conserved hypothetical protein, at 5,131,449 to 5,131,646
_4306
Psyr_4307: Psyr_4307 - Protein of unknown function DUF142, at 5,132,214 to 5,133,149
_4307
Position (kb) 5131
5132 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5130.673 kb on + strand, within Psyr_4304 at 5130.673 kb on + strand, within Psyr_4304 at 5130.674 kb on - strand, within Psyr_4304 at 5130.674 kb on - strand, within Psyr_4304 at 5130.684 kb on + strand, within Psyr_4304 at 5130.685 kb on - strand, within Psyr_4304 at 5130.792 kb on + strand, within Psyr_4304 at 5130.792 kb on + strand, within Psyr_4304 at 5130.793 kb on - strand, within Psyr_4304 at 5130.793 kb on - strand, within Psyr_4304 at 5130.793 kb on - strand, within Psyr_4304 at 5130.801 kb on + strand at 5130.801 kb on + strand at 5130.801 kb on + strand at 5130.801 kb on + strand at 5130.802 kb on - strand at 5130.848 kb on + strand at 5130.848 kb on + strand at 5130.848 kb on + strand at 5130.848 kb on + strand at 5130.848 kb on + strand at 5130.848 kb on + strand at 5130.848 kb on + strand at 5130.848 kb on + strand at 5130.848 kb on + strand at 5130.848 kb on + strand at 5130.849 kb on - strand at 5130.849 kb on - strand at 5130.849 kb on - strand at 5130.849 kb on - strand at 5130.849 kb on - strand at 5130.849 kb on - strand at 5130.849 kb on - strand at 5130.849 kb on - strand at 5130.849 kb on - strand at 5130.849 kb on - strand at 5130.938 kb on + strand at 5130.997 kb on + strand at 5130.997 kb on + strand at 5130.998 kb on - strand at 5130.998 kb on - strand at 5130.998 kb on - strand at 5130.998 kb on - strand at 5131.077 kb on - strand at 5131.077 kb on - strand at 5131.119 kb on + strand at 5131.120 kb on - strand at 5131.120 kb on - strand at 5131.273 kb on - strand, within Psyr_4305 at 5131.408 kb on + strand at 5131.408 kb on + strand at 5131.408 kb on + strand at 5131.408 kb on + strand at 5131.408 kb on + strand at 5131.408 kb on + strand at 5131.409 kb on - strand at 5131.409 kb on - strand at 5131.409 kb on - strand at 5131.409 kb on - strand at 5131.409 kb on - strand at 5131.409 kb on - strand at 5131.409 kb on - strand at 5131.438 kb on + strand at 5131.438 kb on + strand at 5131.438 kb on + strand at 5131.438 kb on + strand at 5131.438 kb on + strand at 5131.438 kb on + strand at 5131.439 kb on - strand at 5131.439 kb on - strand at 5131.439 kb on - strand at 5131.439 kb on - strand at 5131.439 kb on - strand at 5131.439 kb on - strand at 5131.442 kb on + strand at 5131.443 kb on - strand at 5131.443 kb on - strand at 5131.605 kb on - strand, within Psyr_4306 at 5131.638 kb on - strand at 5131.697 kb on - strand at 5131.730 kb on - strand at 5131.730 kb on - strand at 5131.731 kb on + strand at 5131.731 kb on + strand at 5131.731 kb on + strand at 5131.732 kb on - strand at 5131.759 kb on - strand at 5131.759 kb on - strand at 5131.759 kb on - strand at 5131.759 kb on - strand at 5131.759 kb on - strand at 5131.762 kb on + strand at 5131.830 kb on - strand at 5131.830 kb on - strand at 5131.830 kb on - strand at 5131.913 kb on - strand at 5131.913 kb on - strand at 5131.922 kb on + strand at 5131.922 kb on + strand at 5131.922 kb on + strand at 5131.922 kb on + strand at 5131.922 kb on + strand at 5131.929 kb on + strand at 5131.929 kb on + strand at 5131.929 kb on + strand at 5131.930 kb on - strand at 5131.955 kb on + strand at 5131.955 kb on + strand at 5131.955 kb on + strand at 5131.955 kb on + strand at 5131.955 kb on + strand at 5131.956 kb on - strand at 5131.956 kb on - strand at 5131.956 kb on - strand at 5131.956 kb on - strand at 5131.956 kb on - strand at 5131.956 kb on - strand at 5131.963 kb on - strand at 5131.963 kb on - strand at 5131.968 kb on - strand at 5132.105 kb on - strand at 5132.138 kb on + strand at 5132.138 kb on + strand at 5132.139 kb on - strand at 5132.139 kb on - strand at 5132.166 kb on + strand at 5132.166 kb on + strand at 5132.166 kb on + strand at 5132.167 kb on - strand at 5132.167 kb on - strand at 5132.167 kb on - strand at 5132.168 kb on + strand at 5132.169 kb on - strand at 5132.169 kb on - strand at 5132.169 kb on - strand at 5132.169 kb on - strand at 5132.169 kb on - strand at 5132.169 kb on - strand at 5132.172 kb on + strand at 5132.172 kb on + strand at 5132.172 kb on + strand at 5132.173 kb on - strand at 5132.173 kb on - strand at 5132.173 kb on - strand at 5132.173 kb on - strand at 5132.173 kb on - strand at 5132.173 kb on - strand at 5132.174 kb on + strand at 5132.190 kb on + strand at 5132.190 kb on + strand at 5132.190 kb on + strand at 5132.191 kb on - strand at 5132.193 kb on + strand at 5132.193 kb on + strand at 5132.194 kb on - strand at 5132.194 kb on - strand at 5132.194 kb on - strand at 5132.194 kb on - strand at 5132.229 kb on + strand at 5132.230 kb on - strand at 5132.281 kb on + strand at 5132.281 kb on + strand at 5132.281 kb on + strand at 5132.282 kb on - strand at 5132.309 kb on - strand, within Psyr_4307 at 5132.332 kb on - strand, within Psyr_4307 at 5132.333 kb on + strand, within Psyr_4307 at 5132.333 kb on + strand, within Psyr_4307 at 5132.334 kb on - strand, within Psyr_4307 at 5132.417 kb on - strand, within Psyr_4307 at 5132.453 kb on + strand, within Psyr_4307 at 5132.454 kb on - strand, within Psyr_4307 at 5132.515 kb on - strand, within Psyr_4307 at 5132.526 kb on + strand, within Psyr_4307 at 5132.545 kb on + strand, within Psyr_4307 at 5132.545 kb on + strand, within Psyr_4307 at 5132.545 kb on + strand, within Psyr_4307 at 5132.545 kb on + strand, within Psyr_4307 at 5132.545 kb on + strand, within Psyr_4307 at 5132.545 kb on + strand, within Psyr_4307 at 5132.545 kb on + strand, within Psyr_4307 at 5132.545 kb on + strand, within Psyr_4307 at 5132.545 kb on + strand, within Psyr_4307 at 5132.545 kb on + strand, within Psyr_4307 at 5132.545 kb on + strand, within Psyr_4307 at 5132.545 kb on + strand, within Psyr_4307 at 5132.545 kb on + strand, within Psyr_4307 at 5132.545 kb on + strand, within Psyr_4307 at 5132.545 kb on + strand, within Psyr_4307 at 5132.545 kb on + strand, within Psyr_4307 at 5132.545 kb on + strand, within Psyr_4307 at 5132.546 kb on - strand, within Psyr_4307 at 5132.546 kb on - strand, within Psyr_4307 at 5132.546 kb on - strand, within Psyr_4307 at 5132.546 kb on - strand, within Psyr_4307 at 5132.546 kb on - strand, within Psyr_4307 at 5132.546 kb on - strand, within Psyr_4307 at 5132.546 kb on - strand, within Psyr_4307 at 5132.546 kb on - strand, within Psyr_4307 at 5132.546 kb on - strand, within Psyr_4307 at 5132.546 kb on - strand, within Psyr_4307 at 5132.546 kb on - strand, within Psyr_4307 at 5132.546 kb on - strand, within Psyr_4307 at 5132.546 kb on - strand, within Psyr_4307 at 5132.546 kb on - strand, within Psyr_4307 at 5132.546 kb on - strand, within Psyr_4307 at 5132.546 kb on - strand, within Psyr_4307
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 5,130,673 + Psyr_4304 0.79 -0.2 5,130,673 + Psyr_4304 0.79 +1.2 5,130,674 - Psyr_4304 0.79 -0.6 5,130,674 - Psyr_4304 0.79 -0.2 5,130,684 + Psyr_4304 0.80 +0.3 5,130,685 - Psyr_4304 0.80 -0.9 5,130,792 + Psyr_4304 0.90 +2.0 5,130,792 + Psyr_4304 0.90 +0.4 5,130,793 - Psyr_4304 0.90 -0.3 5,130,793 - Psyr_4304 0.90 -0.2 5,130,793 - Psyr_4304 0.90 -1.4 5,130,801 + -0.9 5,130,801 + +0.0 5,130,801 + +0.5 5,130,801 + -0.3 5,130,802 - -0.1 5,130,848 + +0.9 5,130,848 + -0.0 5,130,848 + -1.3 5,130,848 + -0.6 5,130,848 + +0.4 5,130,848 + -0.4 5,130,848 + +0.9 5,130,848 + -0.3 5,130,848 + -1.9 5,130,848 + +0.9 5,130,849 - +0.9 5,130,849 - -1.0 5,130,849 - +0.2 5,130,849 - -0.5 5,130,849 - +0.2 5,130,849 - +0.3 5,130,849 - -1.5 5,130,849 - -0.6 5,130,849 - +0.9 5,130,849 - +0.6 5,130,938 + +0.2 5,130,997 + +0.3 5,130,997 + +0.9 5,130,998 - +1.1 5,130,998 - -0.2 5,130,998 - -0.1 5,130,998 - -0.3 5,131,077 - -0.1 5,131,077 - +1.5 5,131,119 + +0.1 5,131,120 - -0.7 5,131,120 - +0.1 5,131,273 - Psyr_4305 0.71 +0.3 5,131,408 + +1.4 5,131,408 + +1.4 5,131,408 + +0.4 5,131,408 + -1.6 5,131,408 + +0.7 5,131,408 + +1.2 5,131,409 - +0.1 5,131,409 - +0.3 5,131,409 - -0.6 5,131,409 - -0.3 5,131,409 - -1.3 5,131,409 - +0.1 5,131,409 - -0.7 5,131,438 + +0.4 5,131,438 + +1.3 5,131,438 + -0.9 5,131,438 + -0.9 5,131,438 + -0.1 5,131,438 + -1.7 5,131,439 - +0.1 5,131,439 - -0.4 5,131,439 - -1.2 5,131,439 - +0.9 5,131,439 - +0.0 5,131,439 - +0.9 5,131,442 + +0.1 5,131,443 - +0.3 5,131,443 - -0.1 5,131,605 - Psyr_4306 0.79 -0.6 5,131,638 - +0.7 5,131,697 - +0.5 5,131,730 - -0.2 5,131,730 - +1.1 5,131,731 + +0.1 5,131,731 + -1.0 5,131,731 + -2.0 5,131,732 - +1.9 5,131,759 - +0.3 5,131,759 - -0.3 5,131,759 - +1.2 5,131,759 - +2.3 5,131,759 - +0.2 5,131,762 + +0.2 5,131,830 - +1.1 5,131,830 - +0.2 5,131,830 - +0.7 5,131,913 - +0.5 5,131,913 - -1.1 5,131,922 + -0.5 5,131,922 + +0.4 5,131,922 + -0.1 5,131,922 + -0.5 5,131,922 + +0.1 5,131,929 + +0.0 5,131,929 + -0.1 5,131,929 + -0.9 5,131,930 - -0.2 5,131,955 + -0.3 5,131,955 + +1.3 5,131,955 + +0.4 5,131,955 + -1.0 5,131,955 + +0.2 5,131,956 - -0.1 5,131,956 - -0.1 5,131,956 - +0.1 5,131,956 - -0.5 5,131,956 - -0.1 5,131,956 - +0.3 5,131,963 - -0.0 5,131,963 - +0.8 5,131,968 - +0.2 5,132,105 - -0.3 5,132,138 + -1.1 5,132,138 + -2.0 5,132,139 - -1.2 5,132,139 - +0.1 5,132,166 + -0.6 5,132,166 + +1.2 5,132,166 + +0.0 5,132,167 - -1.6 5,132,167 - -0.2 5,132,167 - -0.3 5,132,168 + -0.7 5,132,169 - -0.1 5,132,169 - -2.0 5,132,169 - -0.9 5,132,169 - +0.4 5,132,169 - +0.2 5,132,169 - +0.4 5,132,172 + +0.4 5,132,172 + +0.8 5,132,172 + -1.1 5,132,173 - -0.8 5,132,173 - +0.2 5,132,173 - +1.8 5,132,173 - +1.3 5,132,173 - +0.3 5,132,173 - +0.7 5,132,174 + +1.1 5,132,190 + -0.0 5,132,190 + -0.1 5,132,190 + -0.6 5,132,191 - +0.4 5,132,193 + -2.1 5,132,193 + +0.0 5,132,194 - +0.1 5,132,194 - -0.1 5,132,194 - +0.3 5,132,194 - +0.7 5,132,229 + -1.4 5,132,230 - -1.1 5,132,281 + -1.9 5,132,281 + +0.1 5,132,281 + -0.6 5,132,282 - +0.0 5,132,309 - Psyr_4307 0.10 +1.0 5,132,332 - Psyr_4307 0.13 +0.8 5,132,333 + Psyr_4307 0.13 +0.3 5,132,333 + Psyr_4307 0.13 +0.4 5,132,334 - Psyr_4307 0.13 -0.1 5,132,417 - Psyr_4307 0.22 +0.9 5,132,453 + Psyr_4307 0.26 -2.1 5,132,454 - Psyr_4307 0.26 +1.4 5,132,515 - Psyr_4307 0.32 -0.1 5,132,526 + Psyr_4307 0.33 +0.9 5,132,545 + Psyr_4307 0.35 -0.4 5,132,545 + Psyr_4307 0.35 -0.6 5,132,545 + Psyr_4307 0.35 +1.4 5,132,545 + Psyr_4307 0.35 -0.9 5,132,545 + Psyr_4307 0.35 -0.7 5,132,545 + Psyr_4307 0.35 +1.0 5,132,545 + Psyr_4307 0.35 -1.3 5,132,545 + Psyr_4307 0.35 -1.2 5,132,545 + Psyr_4307 0.35 -1.6 5,132,545 + Psyr_4307 0.35 +0.6 5,132,545 + Psyr_4307 0.35 -0.8 5,132,545 + Psyr_4307 0.35 -1.7 5,132,545 + Psyr_4307 0.35 +0.0 5,132,545 + Psyr_4307 0.35 +0.1 5,132,545 + Psyr_4307 0.35 +0.2 5,132,545 + Psyr_4307 0.35 -0.1 5,132,545 + Psyr_4307 0.35 -1.0 5,132,546 - Psyr_4307 0.35 -0.3 5,132,546 - Psyr_4307 0.35 -0.3 5,132,546 - Psyr_4307 0.35 +0.1 5,132,546 - Psyr_4307 0.35 +0.9 5,132,546 - Psyr_4307 0.35 +0.9 5,132,546 - Psyr_4307 0.35 -0.8 5,132,546 - Psyr_4307 0.35 -0.0 5,132,546 - Psyr_4307 0.35 -0.2 5,132,546 - Psyr_4307 0.35 -1.1 5,132,546 - Psyr_4307 0.35 +0.3 5,132,546 - Psyr_4307 0.35 -1.0 5,132,546 - Psyr_4307 0.35 -0.0 5,132,546 - Psyr_4307 0.35 +0.1 5,132,546 - Psyr_4307 0.35 +2.0 5,132,546 - Psyr_4307 0.35 -0.1 5,132,546 - Psyr_4307 0.35 +0.3
Or see this region's nucleotide sequence