Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4145 and Psyr_4146 are separated by 0 nucleotides Psyr_4146 and Psyr_4147 are separated by 140 nucleotides Psyr_4147 and Psyr_4148 are separated by 75 nucleotides Psyr_4148 and Psyr_4149 are separated by 10 nucleotides Psyr_4149 and Psyr_4150 are separated by 2 nucleotides
Psyr_4145: Psyr_4145 - OstA-like protein, at 4,933,120 to 4,933,668
_4145
Psyr_4146: Psyr_4146 - ABC transporter, at 4,933,669 to 4,934,394
_4146
Psyr_4147: Psyr_4147 - RNA polymerase, sigma 54 subunit, RpoN/SigL, at 4,934,535 to 4,936,070
_4147
Psyr_4148: Psyr_4148 - sigma 54 modulation protein / SSU ribosomal protein S30P, at 4,936,146 to 4,936,454
_4148
Psyr_4149: Psyr_4149 - PTS IIA-like nitrogen-regulatory protein PtsN, at 4,936,465 to 4,936,929
_4149
Psyr_4150: Psyr_4150 - Uncharacterized P-loop ATPase protein UPF0042, at 4,936,932 to 4,937,789
_4150
Position (kb) 4934
4935
4936
4937 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4934.431 kb on + strand at 4934.431 kb on + strand at 4934.432 kb on - strand at 4934.440 kb on + strand at 4934.441 kb on - strand at 4934.455 kb on - strand at 4934.464 kb on - strand at 4934.496 kb on + strand at 4934.498 kb on + strand at 4934.498 kb on + strand at 4934.498 kb on + strand at 4934.507 kb on + strand at 4934.529 kb on + strand at 4934.529 kb on + strand at 4934.529 kb on + strand at 4934.532 kb on + strand at 4934.532 kb on + strand at 4934.532 kb on + strand at 4936.120 kb on + strand at 4936.145 kb on + strand at 4936.145 kb on + strand at 4936.145 kb on + strand at 4936.145 kb on + strand at 4936.200 kb on + strand, within Psyr_4148 at 4936.247 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.357 kb on - strand, within Psyr_4148 at 4936.357 kb on - strand, within Psyr_4148 at 4936.357 kb on - strand, within Psyr_4148 at 4936.357 kb on - strand, within Psyr_4148 at 4936.357 kb on - strand, within Psyr_4148 at 4936.387 kb on + strand, within Psyr_4148 at 4936.387 kb on + strand, within Psyr_4148 at 4936.387 kb on + strand, within Psyr_4148 at 4936.409 kb on + strand, within Psyr_4148 at 4936.463 kb on + strand at 4936.463 kb on + strand at 4936.463 kb on + strand at 4936.463 kb on + strand at 4936.976 kb on + strand at 4937.010 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 4,934,431 + -1.3 4,934,431 + +0.2 4,934,432 - -0.7 4,934,440 + +0.4 4,934,441 - -1.0 4,934,455 - -2.5 4,934,464 - +1.8 4,934,496 + +0.4 4,934,498 + -0.0 4,934,498 + -0.6 4,934,498 + +0.8 4,934,507 + -0.0 4,934,529 + +1.4 4,934,529 + +0.1 4,934,529 + -0.6 4,934,532 + -2.2 4,934,532 + +0.3 4,934,532 + +0.7 4,936,120 + +0.4 4,936,145 + +1.4 4,936,145 + +1.2 4,936,145 + +0.4 4,936,145 + +0.4 4,936,200 + Psyr_4148 0.17 -0.2 4,936,247 + Psyr_4148 0.33 -2.3 4,936,356 + Psyr_4148 0.68 -0.0 4,936,356 + Psyr_4148 0.68 +0.6 4,936,356 + Psyr_4148 0.68 -1.1 4,936,356 + Psyr_4148 0.68 +0.2 4,936,356 + Psyr_4148 0.68 +0.6 4,936,356 + Psyr_4148 0.68 -0.3 4,936,356 + Psyr_4148 0.68 +0.9 4,936,356 + Psyr_4148 0.68 +0.2 4,936,356 + Psyr_4148 0.68 -0.2 4,936,356 + Psyr_4148 0.68 +0.3 4,936,356 + Psyr_4148 0.68 -1.4 4,936,356 + Psyr_4148 0.68 -0.2 4,936,356 + Psyr_4148 0.68 +0.6 4,936,356 + Psyr_4148 0.68 -0.1 4,936,356 + Psyr_4148 0.68 +0.3 4,936,356 + Psyr_4148 0.68 +0.6 4,936,356 + Psyr_4148 0.68 +0.1 4,936,356 + Psyr_4148 0.68 +0.2 4,936,356 + Psyr_4148 0.68 +0.4 4,936,356 + Psyr_4148 0.68 -0.1 4,936,356 + Psyr_4148 0.68 -0.5 4,936,356 + Psyr_4148 0.68 +1.8 4,936,356 + Psyr_4148 0.68 +0.5 4,936,356 + Psyr_4148 0.68 -0.4 4,936,356 + Psyr_4148 0.68 -0.7 4,936,356 + Psyr_4148 0.68 +0.0 4,936,356 + Psyr_4148 0.68 -0.0 4,936,356 + Psyr_4148 0.68 -0.0 4,936,356 + Psyr_4148 0.68 +0.6 4,936,356 + Psyr_4148 0.68 -0.1 4,936,356 + Psyr_4148 0.68 -0.6 4,936,356 + Psyr_4148 0.68 +0.0 4,936,356 + Psyr_4148 0.68 +0.8 4,936,356 + Psyr_4148 0.68 +0.3 4,936,356 + Psyr_4148 0.68 +0.3 4,936,356 + Psyr_4148 0.68 +0.3 4,936,357 - Psyr_4148 0.68 -0.1 4,936,357 - Psyr_4148 0.68 -0.9 4,936,357 - Psyr_4148 0.68 -1.6 4,936,357 - Psyr_4148 0.68 -0.4 4,936,357 - Psyr_4148 0.68 -0.1 4,936,387 + Psyr_4148 0.78 +0.2 4,936,387 + Psyr_4148 0.78 +0.5 4,936,387 + Psyr_4148 0.78 -0.3 4,936,409 + Psyr_4148 0.85 +0.3 4,936,463 + +1.4 4,936,463 + -0.3 4,936,463 + -2.6 4,936,463 + +0.8 4,936,976 + -1.9 4,937,010 + +1.2
Or see this region's nucleotide sequence