Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4016 and Psyr_4017 are separated by 222 nucleotides Psyr_4017 and Psyr_4018 are separated by 77 nucleotides
Psyr_4016: Psyr_4016 - Phosphate transporter, at 4,783,510 to 4,784,982
_4016
Psyr_4017: Psyr_4017 - conserved hypothetical protein, at 4,785,205 to 4,785,405
_4017
Psyr_4018: Psyr_4018 - Formyltetrahydrofolate deformylase, at 4,785,483 to 4,786,334
_4018
Position (kb) 4785
4786 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4784.348 kb on - strand, within Psyr_4016 at 4784.464 kb on + strand, within Psyr_4016 at 4784.693 kb on + strand, within Psyr_4016 at 4784.694 kb on - strand, within Psyr_4016 at 4784.737 kb on + strand, within Psyr_4016 at 4784.738 kb on - strand, within Psyr_4016 at 4785.066 kb on + strand at 4785.066 kb on + strand at 4785.066 kb on + strand at 4785.066 kb on + strand at 4785.075 kb on + strand at 4785.075 kb on + strand at 4785.075 kb on + strand at 4785.075 kb on + strand at 4785.075 kb on + strand at 4785.075 kb on + strand at 4785.149 kb on + strand at 4785.211 kb on - strand at 4785.335 kb on + strand, within Psyr_4017 at 4785.479 kb on + strand at 4785.479 kb on + strand at 4785.479 kb on + strand at 4785.480 kb on - strand at 4785.480 kb on - strand at 4785.480 kb on - strand at 4785.673 kb on + strand, within Psyr_4018 at 4785.673 kb on + strand, within Psyr_4018 at 4785.673 kb on + strand, within Psyr_4018 at 4785.673 kb on + strand, within Psyr_4018 at 4785.673 kb on + strand, within Psyr_4018 at 4785.673 kb on + strand, within Psyr_4018 at 4785.673 kb on + strand, within Psyr_4018 at 4785.673 kb on + strand, within Psyr_4018 at 4785.673 kb on + strand, within Psyr_4018 at 4785.673 kb on + strand, within Psyr_4018 at 4785.673 kb on + strand, within Psyr_4018 at 4785.673 kb on + strand, within Psyr_4018 at 4785.673 kb on + strand, within Psyr_4018 at 4785.673 kb on + strand, within Psyr_4018 at 4785.674 kb on - strand, within Psyr_4018 at 4785.674 kb on - strand, within Psyr_4018 at 4785.674 kb on - strand, within Psyr_4018 at 4785.674 kb on - strand, within Psyr_4018 at 4785.674 kb on - strand, within Psyr_4018 at 4785.674 kb on - strand, within Psyr_4018 at 4785.674 kb on - strand, within Psyr_4018 at 4785.674 kb on - strand, within Psyr_4018 at 4785.724 kb on + strand, within Psyr_4018 at 4785.823 kb on + strand, within Psyr_4018 at 4785.824 kb on - strand, within Psyr_4018 at 4785.824 kb on - strand, within Psyr_4018 at 4785.824 kb on - strand, within Psyr_4018 at 4785.922 kb on + strand, within Psyr_4018 at 4785.922 kb on + strand, within Psyr_4018 at 4785.922 kb on + strand, within Psyr_4018 at 4785.922 kb on + strand, within Psyr_4018 at 4785.922 kb on + strand, within Psyr_4018 at 4785.922 kb on + strand, within Psyr_4018 at 4785.922 kb on + strand, within Psyr_4018 at 4785.922 kb on + strand, within Psyr_4018 at 4785.922 kb on + strand, within Psyr_4018 at 4785.923 kb on - strand, within Psyr_4018 at 4785.923 kb on - strand, within Psyr_4018 at 4785.925 kb on + strand, within Psyr_4018 at 4785.925 kb on + strand, within Psyr_4018 at 4785.925 kb on + strand, within Psyr_4018 at 4785.926 kb on - strand, within Psyr_4018 at 4786.293 kb on + strand at 4786.293 kb on + strand at 4786.293 kb on + strand at 4786.294 kb on - strand at 4786.294 kb on - strand at 4786.371 kb on + strand at 4786.372 kb on - strand at 4786.396 kb on + strand at 4786.396 kb on + strand at 4786.396 kb on + strand at 4786.397 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 4,784,348 - Psyr_4016 0.57 -1.2 4,784,464 + Psyr_4016 0.65 +0.9 4,784,693 + Psyr_4016 0.80 -0.2 4,784,694 - Psyr_4016 0.80 -1.2 4,784,737 + Psyr_4016 0.83 -2.2 4,784,738 - Psyr_4016 0.83 +0.4 4,785,066 + +2.0 4,785,066 + +0.5 4,785,066 + +0.1 4,785,066 + -0.9 4,785,075 + +0.3 4,785,075 + +0.4 4,785,075 + +0.2 4,785,075 + -0.7 4,785,075 + -0.9 4,785,075 + -0.3 4,785,149 + +0.2 4,785,211 - -1.4 4,785,335 + Psyr_4017 0.65 -0.5 4,785,479 + -0.8 4,785,479 + -0.2 4,785,479 + +0.5 4,785,480 - -0.3 4,785,480 - +0.1 4,785,480 - -0.9 4,785,673 + Psyr_4018 0.22 +0.9 4,785,673 + Psyr_4018 0.22 +0.2 4,785,673 + Psyr_4018 0.22 -0.7 4,785,673 + Psyr_4018 0.22 +0.0 4,785,673 + Psyr_4018 0.22 -0.8 4,785,673 + Psyr_4018 0.22 -0.1 4,785,673 + Psyr_4018 0.22 -0.1 4,785,673 + Psyr_4018 0.22 +0.0 4,785,673 + Psyr_4018 0.22 +0.2 4,785,673 + Psyr_4018 0.22 -1.3 4,785,673 + Psyr_4018 0.22 -1.5 4,785,673 + Psyr_4018 0.22 +0.4 4,785,673 + Psyr_4018 0.22 -1.2 4,785,673 + Psyr_4018 0.22 -0.5 4,785,674 - Psyr_4018 0.22 +1.1 4,785,674 - Psyr_4018 0.22 -0.2 4,785,674 - Psyr_4018 0.22 -0.0 4,785,674 - Psyr_4018 0.22 +0.0 4,785,674 - Psyr_4018 0.22 -0.3 4,785,674 - Psyr_4018 0.22 -1.5 4,785,674 - Psyr_4018 0.22 +0.6 4,785,674 - Psyr_4018 0.22 -2.8 4,785,724 + Psyr_4018 0.28 +0.1 4,785,823 + Psyr_4018 0.40 +0.3 4,785,824 - Psyr_4018 0.40 +0.1 4,785,824 - Psyr_4018 0.40 -0.6 4,785,824 - Psyr_4018 0.40 -0.1 4,785,922 + Psyr_4018 0.52 -0.0 4,785,922 + Psyr_4018 0.52 +0.3 4,785,922 + Psyr_4018 0.52 +2.2 4,785,922 + Psyr_4018 0.52 -1.6 4,785,922 + Psyr_4018 0.52 +1.1 4,785,922 + Psyr_4018 0.52 -0.9 4,785,922 + Psyr_4018 0.52 -0.3 4,785,922 + Psyr_4018 0.52 -0.8 4,785,922 + Psyr_4018 0.52 +0.1 4,785,923 - Psyr_4018 0.52 +0.6 4,785,923 - Psyr_4018 0.52 +0.2 4,785,925 + Psyr_4018 0.52 +0.4 4,785,925 + Psyr_4018 0.52 +1.4 4,785,925 + Psyr_4018 0.52 -0.6 4,785,926 - Psyr_4018 0.52 +0.1 4,786,293 + -1.0 4,786,293 + -0.4 4,786,293 + -0.4 4,786,294 - +0.6 4,786,294 - -0.3 4,786,371 + +1.1 4,786,372 - -0.4 4,786,396 + +0.4 4,786,396 + -0.2 4,786,396 + -0.3 4,786,397 - -2.2
Or see this region's nucleotide sequence