Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3859 and Psyr_3860 are separated by 66 nucleotides Psyr_3860 and Psyr_3861 overlap by 8 nucleotides Psyr_3861 and Psyr_3862 are separated by 220 nucleotides Psyr_3862 and Psyr_3863 are separated by 3 nucleotides
Psyr_3859: Psyr_3859 - Purine nucleoside permease, at 4,599,380 to 4,600,411
_3859
Psyr_3860: Psyr_3860 - conserved hypothetical protein, at 4,600,478 to 4,601,236
_3860
Psyr_3861: Psyr_3861 - Protein of unknown function DUF1145, at 4,601,229 to 4,601,504
_3861
Psyr_3862: Psyr_3862 - conserved hypothetical protein, at 4,601,725 to 4,602,222
_3862
Psyr_3863: Psyr_3863 - SEC-C motif protein, at 4,602,226 to 4,602,699
_3863
Position (kb) 4601
4602 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4600.296 kb on - strand, within Psyr_3859 at 4600.409 kb on + strand at 4600.479 kb on + strand at 4600.480 kb on - strand at 4600.579 kb on + strand, within Psyr_3860 at 4600.579 kb on + strand, within Psyr_3860 at 4600.750 kb on + strand, within Psyr_3860 at 4600.752 kb on + strand, within Psyr_3860 at 4600.752 kb on + strand, within Psyr_3860 at 4600.753 kb on - strand, within Psyr_3860 at 4600.819 kb on - strand, within Psyr_3860 at 4600.990 kb on + strand, within Psyr_3860 at 4600.990 kb on + strand, within Psyr_3860 at 4600.991 kb on - strand, within Psyr_3860 at 4600.991 kb on - strand, within Psyr_3860 at 4600.991 kb on - strand, within Psyr_3860 at 4600.991 kb on - strand, within Psyr_3860 at 4600.991 kb on - strand, within Psyr_3860 at 4601.176 kb on + strand at 4601.176 kb on + strand at 4601.177 kb on - strand at 4601.177 kb on - strand at 4601.230 kb on + strand at 4601.231 kb on - strand at 4601.231 kb on - strand at 4601.475 kb on + strand, within Psyr_3861 at 4601.575 kb on - strand at 4601.586 kb on - strand at 4601.626 kb on + strand at 4601.626 kb on + strand at 4601.626 kb on + strand at 4601.626 kb on + strand at 4601.626 kb on + strand at 4601.626 kb on + strand at 4601.626 kb on + strand at 4601.626 kb on + strand at 4601.626 kb on + strand at 4601.626 kb on + strand at 4601.627 kb on - strand at 4601.627 kb on - strand at 4601.627 kb on - strand at 4601.627 kb on - strand at 4601.627 kb on - strand at 4601.627 kb on - strand at 4601.627 kb on - strand at 4601.627 kb on - strand at 4601.627 kb on - strand at 4601.627 kb on - strand at 4601.627 kb on - strand at 4601.627 kb on - strand at 4601.696 kb on - strand at 4601.749 kb on - strand at 4601.749 kb on - strand at 4602.129 kb on + strand, within Psyr_3862 at 4602.129 kb on + strand, within Psyr_3862 at 4602.133 kb on + strand, within Psyr_3862 at 4602.134 kb on - strand, within Psyr_3862 at 4602.134 kb on - strand, within Psyr_3862 at 4602.148 kb on + strand, within Psyr_3862 at 4602.181 kb on + strand at 4602.182 kb on - strand at 4602.182 kb on - strand at 4602.212 kb on - strand at 4602.212 kb on - strand at 4602.212 kb on - strand at 4602.212 kb on - strand at 4602.225 kb on + strand at 4602.225 kb on + strand at 4602.225 kb on + strand at 4602.225 kb on + strand at 4602.225 kb on + strand at 4602.225 kb on + strand at 4602.225 kb on + strand at 4602.225 kb on + strand at 4602.226 kb on - strand at 4602.226 kb on - strand at 4602.226 kb on - strand at 4602.226 kb on - strand at 4602.231 kb on + strand at 4602.338 kb on - strand, within Psyr_3863 at 4602.346 kb on + strand, within Psyr_3863 at 4602.346 kb on + strand, within Psyr_3863 at 4602.347 kb on - strand, within Psyr_3863 at 4602.347 kb on - strand, within Psyr_3863 at 4602.347 kb on - strand, within Psyr_3863 at 4602.381 kb on + strand, within Psyr_3863 at 4602.381 kb on + strand, within Psyr_3863 at 4602.381 kb on + strand, within Psyr_3863 at 4602.382 kb on - strand, within Psyr_3863 at 4602.464 kb on + strand, within Psyr_3863 at 4602.465 kb on - strand, within Psyr_3863 at 4602.465 kb on - strand, within Psyr_3863
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 4,600,296 - Psyr_3859 0.89 +0.2 4,600,409 + -1.5 4,600,479 + -0.7 4,600,480 - -2.6 4,600,579 + Psyr_3860 0.13 +0.3 4,600,579 + Psyr_3860 0.13 +0.1 4,600,750 + Psyr_3860 0.36 +0.6 4,600,752 + Psyr_3860 0.36 -0.3 4,600,752 + Psyr_3860 0.36 +1.3 4,600,753 - Psyr_3860 0.36 -2.3 4,600,819 - Psyr_3860 0.45 -0.3 4,600,990 + Psyr_3860 0.67 -0.3 4,600,990 + Psyr_3860 0.67 +0.2 4,600,991 - Psyr_3860 0.68 -1.5 4,600,991 - Psyr_3860 0.68 +0.9 4,600,991 - Psyr_3860 0.68 -0.7 4,600,991 - Psyr_3860 0.68 -0.2 4,600,991 - Psyr_3860 0.68 -1.2 4,601,176 + +0.4 4,601,176 + +0.6 4,601,177 - -0.6 4,601,177 - +1.2 4,601,230 + +1.1 4,601,231 - -0.6 4,601,231 - +0.6 4,601,475 + Psyr_3861 0.89 -0.2 4,601,575 - +0.4 4,601,586 - -0.7 4,601,626 + +0.7 4,601,626 + -0.8 4,601,626 + -0.7 4,601,626 + -0.6 4,601,626 + -0.2 4,601,626 + +0.2 4,601,626 + -0.4 4,601,626 + -0.9 4,601,626 + -1.8 4,601,626 + -0.9 4,601,627 - -1.9 4,601,627 - -0.7 4,601,627 - -1.6 4,601,627 - -1.2 4,601,627 - +0.5 4,601,627 - -0.3 4,601,627 - +3.4 4,601,627 - +0.7 4,601,627 - +0.5 4,601,627 - -1.1 4,601,627 - +0.4 4,601,627 - -0.3 4,601,696 - -1.6 4,601,749 - -0.1 4,601,749 - -0.5 4,602,129 + Psyr_3862 0.81 +0.2 4,602,129 + Psyr_3862 0.81 -0.1 4,602,133 + Psyr_3862 0.82 -0.0 4,602,134 - Psyr_3862 0.82 +0.3 4,602,134 - Psyr_3862 0.82 -0.3 4,602,148 + Psyr_3862 0.85 -0.5 4,602,181 + -1.2 4,602,182 - +0.4 4,602,182 - +0.8 4,602,212 - -0.3 4,602,212 - +1.0 4,602,212 - -1.1 4,602,212 - +0.2 4,602,225 + +0.7 4,602,225 + +0.4 4,602,225 + +1.0 4,602,225 + +0.7 4,602,225 + +0.1 4,602,225 + +0.3 4,602,225 + -0.0 4,602,225 + +1.1 4,602,226 - -1.0 4,602,226 - -1.1 4,602,226 - +0.1 4,602,226 - -1.4 4,602,231 + -1.5 4,602,338 - Psyr_3863 0.24 -1.8 4,602,346 + Psyr_3863 0.25 -0.2 4,602,346 + Psyr_3863 0.25 -1.6 4,602,347 - Psyr_3863 0.26 -2.0 4,602,347 - Psyr_3863 0.26 -0.4 4,602,347 - Psyr_3863 0.26 +0.2 4,602,381 + Psyr_3863 0.33 +0.5 4,602,381 + Psyr_3863 0.33 +0.4 4,602,381 + Psyr_3863 0.33 +0.7 4,602,382 - Psyr_3863 0.33 -1.2 4,602,464 + Psyr_3863 0.50 -0.6 4,602,465 - Psyr_3863 0.50 +0.5 4,602,465 - Psyr_3863 0.50 -1.7
Or see this region's nucleotide sequence