Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3749 and Psyr_3750 are separated by 16 nucleotides Psyr_3750 and Psyr_3751 are separated by 56 nucleotides Psyr_3751 and Psyr_3752 are separated by 170 nucleotides
Psyr_3749: Psyr_3749 - Peptidase M42, at 4,468,065 to 4,469,261
_3749
Psyr_3750: Psyr_3750 - Protein of unknown function UPF0270, at 4,469,278 to 4,469,556
_3750
Psyr_3751: Psyr_3751 - Carbon storage regulator, at 4,469,613 to 4,469,807
_3751
Psyr_3752: Psyr_3752 - Protein of unknown function UPF0060, at 4,469,978 to 4,470,310
_3752
Position (kb)
4469
4470 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4468.323 kb on - strand, within Psyr_3749 at 4468.524 kb on + strand, within Psyr_3749 at 4468.524 kb on + strand, within Psyr_3749 at 4468.881 kb on + strand, within Psyr_3749 at 4468.962 kb on + strand, within Psyr_3749 at 4468.962 kb on + strand, within Psyr_3749 at 4468.962 kb on + strand, within Psyr_3749 at 4468.965 kb on + strand, within Psyr_3749 at 4468.965 kb on + strand, within Psyr_3749 at 4468.965 kb on + strand, within Psyr_3749 at 4468.966 kb on - strand, within Psyr_3749 at 4468.966 kb on - strand, within Psyr_3749 at 4468.966 kb on - strand, within Psyr_3749 at 4468.966 kb on - strand, within Psyr_3749 at 4468.966 kb on - strand, within Psyr_3749 at 4468.966 kb on - strand, within Psyr_3749 at 4468.966 kb on - strand, within Psyr_3749 at 4469.103 kb on + strand, within Psyr_3749 at 4469.103 kb on + strand, within Psyr_3749 at 4469.103 kb on + strand, within Psyr_3749 at 4469.103 kb on + strand, within Psyr_3749 at 4469.103 kb on + strand, within Psyr_3749 at 4469.103 kb on + strand, within Psyr_3749 at 4469.103 kb on + strand, within Psyr_3749 at 4469.103 kb on + strand, within Psyr_3749 at 4469.103 kb on + strand, within Psyr_3749 at 4469.103 kb on + strand, within Psyr_3749 at 4469.103 kb on + strand, within Psyr_3749 at 4469.104 kb on - strand at 4469.104 kb on - strand, within Psyr_3749 at 4469.104 kb on - strand, within Psyr_3749 at 4469.104 kb on - strand, within Psyr_3749 at 4469.104 kb on - strand, within Psyr_3749 at 4469.104 kb on - strand, within Psyr_3749 at 4469.107 kb on + strand, within Psyr_3749 at 4469.107 kb on + strand, within Psyr_3749 at 4469.107 kb on + strand, within Psyr_3749 at 4469.107 kb on + strand, within Psyr_3749 at 4469.107 kb on + strand, within Psyr_3749 at 4469.107 kb on + strand, within Psyr_3749 at 4469.108 kb on - strand, within Psyr_3749 at 4469.108 kb on - strand, within Psyr_3749 at 4469.108 kb on - strand, within Psyr_3749 at 4469.108 kb on - strand, within Psyr_3749 at 4469.108 kb on - strand, within Psyr_3749 at 4469.108 kb on - strand, within Psyr_3749 at 4469.297 kb on + strand at 4469.297 kb on + strand at 4469.297 kb on + strand at 4469.297 kb on + strand at 4469.297 kb on + strand at 4469.297 kb on + strand at 4469.297 kb on + strand at 4469.297 kb on + strand at 4469.298 kb on - strand at 4469.298 kb on - strand at 4469.298 kb on - strand at 4469.298 kb on - strand at 4469.298 kb on - strand at 4469.442 kb on - strand, within Psyr_3750 at 4469.561 kb on + strand at 4469.561 kb on + strand at 4469.562 kb on - strand at 4469.562 kb on - strand at 4469.665 kb on + strand, within Psyr_3751 at 4469.665 kb on + strand, within Psyr_3751 at 4469.665 kb on + strand, within Psyr_3751 at 4469.666 kb on - strand, within Psyr_3751 at 4469.666 kb on - strand, within Psyr_3751 at 4469.666 kb on - strand, within Psyr_3751 at 4469.666 kb on - strand, within Psyr_3751 at 4469.800 kb on - strand at 4469.888 kb on - strand at 4469.930 kb on + strand at 4469.931 kb on - strand at 4469.957 kb on - strand at 4470.111 kb on + strand, within Psyr_3752 at 4470.111 kb on + strand, within Psyr_3752 at 4470.111 kb on + strand, within Psyr_3752 at 4470.111 kb on + strand, within Psyr_3752 at 4470.111 kb on + strand, within Psyr_3752 at 4470.112 kb on - strand, within Psyr_3752 at 4470.112 kb on - strand, within Psyr_3752 at 4470.112 kb on - strand, within Psyr_3752 at 4470.112 kb on - strand, within Psyr_3752 at 4470.112 kb on - strand, within Psyr_3752 at 4470.112 kb on - strand, within Psyr_3752 at 4470.112 kb on - strand, within Psyr_3752 at 4470.112 kb on - strand, within Psyr_3752 at 4470.112 kb on - strand, within Psyr_3752 at 4470.112 kb on - strand, within Psyr_3752 at 4470.112 kb on - strand, within Psyr_3752 at 4470.124 kb on - strand, within Psyr_3752 at 4470.236 kb on + strand, within Psyr_3752 at 4470.237 kb on - strand, within Psyr_3752 at 4470.255 kb on + strand, within Psyr_3752 at 4470.255 kb on + strand, within Psyr_3752 at 4470.256 kb on - strand, within Psyr_3752 at 4470.256 kb on - strand, within Psyr_3752 at 4470.256 kb on - strand, within Psyr_3752 at 4470.256 kb on - strand, within Psyr_3752 at 4470.256 kb on - strand, within Psyr_3752 at 4470.266 kb on + strand, within Psyr_3752 at 4470.267 kb on - strand, within Psyr_3752 at 4470.300 kb on + strand at 4470.300 kb on + strand at 4470.300 kb on + strand at 4470.300 kb on + strand at 4470.413 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 4,468,323 - Psyr_3749 0.22 +0.8 4,468,524 + Psyr_3749 0.38 -0.2 4,468,524 + Psyr_3749 0.38 +0.1 4,468,881 + Psyr_3749 0.68 -0.2 4,468,962 + Psyr_3749 0.75 +2.4 4,468,962 + Psyr_3749 0.75 -0.2 4,468,962 + Psyr_3749 0.75 +0.8 4,468,965 + Psyr_3749 0.75 +0.3 4,468,965 + Psyr_3749 0.75 -1.5 4,468,965 + Psyr_3749 0.75 -0.1 4,468,966 - Psyr_3749 0.75 -0.1 4,468,966 - Psyr_3749 0.75 -0.7 4,468,966 - Psyr_3749 0.75 +0.1 4,468,966 - Psyr_3749 0.75 -0.3 4,468,966 - Psyr_3749 0.75 +2.4 4,468,966 - Psyr_3749 0.75 +0.3 4,468,966 - Psyr_3749 0.75 -1.0 4,469,103 + Psyr_3749 0.87 -1.6 4,469,103 + Psyr_3749 0.87 -0.1 4,469,103 + Psyr_3749 0.87 +1.3 4,469,103 + Psyr_3749 0.87 -0.9 4,469,103 + Psyr_3749 0.87 -0.6 4,469,103 + Psyr_3749 0.87 +0.2 4,469,103 + Psyr_3749 0.87 -0.3 4,469,103 + Psyr_3749 0.87 -0.1 4,469,103 + Psyr_3749 0.87 +3.4 4,469,103 + Psyr_3749 0.87 -0.6 4,469,103 + Psyr_3749 0.87 +0.2 4,469,104 - -0.2 4,469,104 - Psyr_3749 0.87 -0.5 4,469,104 - Psyr_3749 0.87 -0.3 4,469,104 - Psyr_3749 0.87 +0.6 4,469,104 - Psyr_3749 0.87 +0.7 4,469,104 - Psyr_3749 0.87 -0.3 4,469,107 + Psyr_3749 0.87 -0.8 4,469,107 + Psyr_3749 0.87 -0.1 4,469,107 + Psyr_3749 0.87 +0.5 4,469,107 + Psyr_3749 0.87 -1.6 4,469,107 + Psyr_3749 0.87 +0.2 4,469,107 + Psyr_3749 0.87 +1.1 4,469,108 - Psyr_3749 0.87 +1.2 4,469,108 - Psyr_3749 0.87 +0.9 4,469,108 - Psyr_3749 0.87 +0.4 4,469,108 - Psyr_3749 0.87 +0.4 4,469,108 - Psyr_3749 0.87 +1.5 4,469,108 - Psyr_3749 0.87 +0.0 4,469,297 + -0.3 4,469,297 + -0.3 4,469,297 + -0.1 4,469,297 + +1.0 4,469,297 + -0.2 4,469,297 + +1.2 4,469,297 + -0.2 4,469,297 + -0.1 4,469,298 - +0.5 4,469,298 - -0.9 4,469,298 - +0.3 4,469,298 - +0.4 4,469,298 - +0.3 4,469,442 - Psyr_3750 0.59 +0.5 4,469,561 + +0.1 4,469,561 + +0.2 4,469,562 - +1.1 4,469,562 - -0.5 4,469,665 + Psyr_3751 0.27 -0.3 4,469,665 + Psyr_3751 0.27 -0.2 4,469,665 + Psyr_3751 0.27 -1.1 4,469,666 - Psyr_3751 0.27 +0.1 4,469,666 - Psyr_3751 0.27 -0.1 4,469,666 - Psyr_3751 0.27 -0.1 4,469,666 - Psyr_3751 0.27 -0.4 4,469,800 - +1.3 4,469,888 - -0.2 4,469,930 + -0.6 4,469,931 - -0.3 4,469,957 - +1.2 4,470,111 + Psyr_3752 0.40 +0.7 4,470,111 + Psyr_3752 0.40 -2.5 4,470,111 + Psyr_3752 0.40 -1.4 4,470,111 + Psyr_3752 0.40 -1.7 4,470,111 + Psyr_3752 0.40 -0.7 4,470,112 - Psyr_3752 0.40 -0.1 4,470,112 - Psyr_3752 0.40 +0.3 4,470,112 - Psyr_3752 0.40 +0.2 4,470,112 - Psyr_3752 0.40 +1.7 4,470,112 - Psyr_3752 0.40 -0.9 4,470,112 - Psyr_3752 0.40 +0.4 4,470,112 - Psyr_3752 0.40 -0.7 4,470,112 - Psyr_3752 0.40 -1.3 4,470,112 - Psyr_3752 0.40 -0.4 4,470,112 - Psyr_3752 0.40 +0.5 4,470,112 - Psyr_3752 0.40 +0.7 4,470,124 - Psyr_3752 0.44 +0.3 4,470,236 + Psyr_3752 0.77 +1.8 4,470,237 - Psyr_3752 0.78 -0.8 4,470,255 + Psyr_3752 0.83 +0.6 4,470,255 + Psyr_3752 0.83 -0.2 4,470,256 - Psyr_3752 0.83 -0.9 4,470,256 - Psyr_3752 0.83 -0.0 4,470,256 - Psyr_3752 0.83 +0.4 4,470,256 - Psyr_3752 0.83 +0.2 4,470,256 - Psyr_3752 0.83 +0.5 4,470,266 + Psyr_3752 0.86 +0.4 4,470,267 - Psyr_3752 0.87 +0.2 4,470,300 + +0.8 4,470,300 + +0.2 4,470,300 + +0.4 4,470,300 + -0.1 4,470,413 + -0.3
Or see this region's nucleotide sequence