Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3700 and Psyr_3701 are separated by 170 nucleotides Psyr_3701 and Psyr_3702 are separated by 128 nucleotides Psyr_3702 and Psyr_3703 overlap by 4 nucleotides Psyr_3703 and Psyr_3704 are separated by 99 nucleotides
Psyr_3700: Psyr_3700 - Protein of unknown function DUF306, at 4,388,818 to 4,389,225
_3700
Psyr_3701: Psyr_3701 - thioredoxin, putative, at 4,389,396 to 4,389,863
_3701
Psyr_3702: Psyr_3702 - Arsenate reductase, at 4,389,992 to 4,390,345
_3702
Psyr_3703: Psyr_3703 - Flavodoxin/nitric oxide synthase, at 4,390,342 to 4,390,947
_3703
Psyr_3704: Psyr_3704 - conserved hypothetical protein, at 4,391,047 to 4,391,718
_3704
Position (kb) 4389
4390
4391 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4389.027 kb on + strand, within Psyr_3700 at 4389.027 kb on + strand, within Psyr_3700 at 4389.118 kb on - strand, within Psyr_3700 at 4389.118 kb on - strand, within Psyr_3700 at 4389.150 kb on + strand, within Psyr_3700 at 4389.219 kb on + strand at 4389.219 kb on + strand at 4389.220 kb on - strand at 4389.220 kb on - strand at 4389.229 kb on + strand at 4389.229 kb on + strand at 4389.229 kb on + strand at 4389.229 kb on + strand at 4389.230 kb on - strand at 4389.230 kb on - strand at 4389.230 kb on - strand at 4389.233 kb on + strand at 4389.233 kb on + strand at 4389.234 kb on - strand at 4389.234 kb on - strand at 4389.254 kb on - strand at 4389.254 kb on - strand at 4389.370 kb on - strand at 4389.496 kb on + strand, within Psyr_3701 at 4389.497 kb on - strand, within Psyr_3701 at 4389.497 kb on - strand, within Psyr_3701 at 4389.497 kb on - strand, within Psyr_3701 at 4389.497 kb on - strand, within Psyr_3701 at 4389.533 kb on - strand, within Psyr_3701 at 4389.725 kb on - strand, within Psyr_3701 at 4389.897 kb on + strand at 4389.897 kb on + strand at 4389.897 kb on + strand at 4389.897 kb on + strand at 4389.897 kb on + strand at 4389.897 kb on + strand at 4389.897 kb on + strand at 4389.898 kb on - strand at 4389.898 kb on - strand at 4389.898 kb on - strand at 4389.898 kb on - strand at 4389.898 kb on - strand at 4389.898 kb on - strand at 4389.901 kb on + strand at 4389.901 kb on + strand at 4389.901 kb on + strand at 4389.901 kb on + strand at 4389.901 kb on + strand at 4389.901 kb on + strand at 4389.901 kb on + strand at 4389.902 kb on - strand at 4389.902 kb on - strand at 4389.904 kb on - strand at 4389.918 kb on + strand at 4389.933 kb on - strand at 4389.957 kb on + strand at 4389.962 kb on + strand at 4389.962 kb on + strand at 4390.010 kb on + strand at 4390.187 kb on + strand, within Psyr_3702 at 4390.187 kb on + strand, within Psyr_3702 at 4390.187 kb on + strand, within Psyr_3702 at 4390.188 kb on - strand, within Psyr_3702 at 4390.354 kb on + strand at 4390.354 kb on + strand at 4390.354 kb on + strand at 4390.354 kb on + strand at 4390.354 kb on + strand at 4390.354 kb on + strand at 4390.354 kb on + strand at 4390.354 kb on + strand at 4390.354 kb on + strand at 4390.355 kb on - strand at 4390.355 kb on - strand at 4390.355 kb on - strand at 4390.355 kb on - strand at 4390.355 kb on - strand at 4390.369 kb on + strand at 4390.369 kb on + strand at 4390.370 kb on - strand at 4390.370 kb on - strand at 4390.372 kb on + strand at 4390.373 kb on - strand at 4390.522 kb on + strand, within Psyr_3703 at 4390.609 kb on + strand, within Psyr_3703 at 4390.609 kb on + strand, within Psyr_3703 at 4390.609 kb on + strand, within Psyr_3703 at 4390.610 kb on - strand, within Psyr_3703 at 4390.610 kb on - strand, within Psyr_3703 at 4390.610 kb on - strand, within Psyr_3703 at 4390.677 kb on + strand, within Psyr_3703 at 4391.056 kb on + strand at 4391.056 kb on + strand at 4391.074 kb on + strand at 4391.074 kb on + strand at 4391.178 kb on + strand, within Psyr_3704 at 4391.179 kb on - strand, within Psyr_3704 at 4391.179 kb on - strand, within Psyr_3704
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 4,389,027 + Psyr_3700 0.51 -0.3 4,389,027 + Psyr_3700 0.51 -0.1 4,389,118 - Psyr_3700 0.74 -1.2 4,389,118 - Psyr_3700 0.74 +0.2 4,389,150 + Psyr_3700 0.81 +0.6 4,389,219 + +0.7 4,389,219 + -0.6 4,389,220 - -1.4 4,389,220 - -1.4 4,389,229 + +0.1 4,389,229 + -1.2 4,389,229 + -0.8 4,389,229 + +0.4 4,389,230 - -1.2 4,389,230 - -2.4 4,389,230 - +0.4 4,389,233 + +0.1 4,389,233 + +0.0 4,389,234 - -0.1 4,389,234 - -2.4 4,389,254 - +0.1 4,389,254 - +0.1 4,389,370 - -0.2 4,389,496 + Psyr_3701 0.21 +0.4 4,389,497 - Psyr_3701 0.22 -1.2 4,389,497 - Psyr_3701 0.22 +0.4 4,389,497 - Psyr_3701 0.22 -0.7 4,389,497 - Psyr_3701 0.22 -0.4 4,389,533 - Psyr_3701 0.29 +0.4 4,389,725 - Psyr_3701 0.70 -1.2 4,389,897 + +0.6 4,389,897 + +0.2 4,389,897 + +0.5 4,389,897 + -0.1 4,389,897 + -1.7 4,389,897 + -0.6 4,389,897 + +0.7 4,389,898 - +0.3 4,389,898 - +1.2 4,389,898 - -0.0 4,389,898 - +0.1 4,389,898 - -0.7 4,389,898 - -0.9 4,389,901 + +1.3 4,389,901 + -0.3 4,389,901 + -0.5 4,389,901 + -0.0 4,389,901 + -0.6 4,389,901 + +1.0 4,389,901 + -0.8 4,389,902 - -0.4 4,389,902 - -0.1 4,389,904 - -0.9 4,389,918 + +0.1 4,389,933 - -0.8 4,389,957 + +0.6 4,389,962 + -0.8 4,389,962 + -1.9 4,390,010 + +2.0 4,390,187 + Psyr_3702 0.55 -0.9 4,390,187 + Psyr_3702 0.55 +0.5 4,390,187 + Psyr_3702 0.55 -1.9 4,390,188 - Psyr_3702 0.55 -1.0 4,390,354 + -0.8 4,390,354 + -0.2 4,390,354 + +0.3 4,390,354 + -0.8 4,390,354 + -0.9 4,390,354 + -1.0 4,390,354 + +0.7 4,390,354 + -0.3 4,390,354 + +0.7 4,390,355 - +0.8 4,390,355 - -0.3 4,390,355 - -0.1 4,390,355 - +0.7 4,390,355 - +0.5 4,390,369 + +0.5 4,390,369 + +0.4 4,390,370 - +0.5 4,390,370 - -0.9 4,390,372 + +0.6 4,390,373 - -0.5 4,390,522 + Psyr_3703 0.30 -0.8 4,390,609 + Psyr_3703 0.44 +1.2 4,390,609 + Psyr_3703 0.44 -0.1 4,390,609 + Psyr_3703 0.44 -0.5 4,390,610 - Psyr_3703 0.44 -1.4 4,390,610 - Psyr_3703 0.44 -0.0 4,390,610 - Psyr_3703 0.44 +0.5 4,390,677 + Psyr_3703 0.55 -0.3 4,391,056 + -1.2 4,391,056 + +1.1 4,391,074 + -0.5 4,391,074 + -1.1 4,391,178 + Psyr_3704 0.19 -0.0 4,391,179 - Psyr_3704 0.20 +1.2 4,391,179 - Psyr_3704 0.20 +0.9
Or see this region's nucleotide sequence