Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3640 and Psyr_3641 are separated by 24 nucleotides Psyr_3641 and Psyr_3642 are separated by 320 nucleotides Psyr_3642 and Psyr_3643 are separated by 121 nucleotides Psyr_3643 and Psyr_3644 overlap by 4 nucleotides
Psyr_3640: Psyr_3640 - conserved hypothetical protein, at 4,323,108 to 4,323,350
_3640
Psyr_3641: Psyr_3641 - Integration host factor, beta subunit, at 4,323,375 to 4,323,671
_3641
Psyr_3642: Psyr_3642 - SSU ribosomal protein S1P, at 4,323,992 to 4,325,683
_3642
Psyr_3643: Psyr_3643 - cytidylate kinase, at 4,325,805 to 4,326,494
_3643
Psyr_3644: Psyr_3644 - prephenate dehydrogenase, at 4,326,491 to 4,328,095
_3644
Position (kb) 4323
4324
4325
4326 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4323.279 kb on + strand, within Psyr_3640 at 4323.279 kb on + strand, within Psyr_3640 at 4323.279 kb on + strand, within Psyr_3640 at 4323.280 kb on - strand, within Psyr_3640 at 4323.280 kb on - strand, within Psyr_3640 at 4323.280 kb on - strand, within Psyr_3640 at 4323.344 kb on + strand at 4323.344 kb on + strand at 4323.344 kb on + strand at 4323.345 kb on - strand at 4323.345 kb on - strand at 4323.376 kb on + strand at 4323.698 kb on - strand at 4323.698 kb on - strand at 4323.751 kb on + strand at 4323.751 kb on + strand at 4323.751 kb on + strand at 4323.751 kb on + strand at 4323.751 kb on + strand at 4323.751 kb on + strand at 4323.759 kb on + strand at 4323.759 kb on + strand at 4323.759 kb on + strand at 4323.759 kb on + strand at 4323.760 kb on - strand at 4323.760 kb on - strand at 4323.760 kb on - strand at 4323.848 kb on + strand at 4323.848 kb on + strand at 4323.849 kb on - strand at 4326.587 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 4,323,279 + Psyr_3640 0.70 -0.6 4,323,279 + Psyr_3640 0.70 +1.9 4,323,279 + Psyr_3640 0.70 +1.4 4,323,280 - Psyr_3640 0.71 -1.5 4,323,280 - Psyr_3640 0.71 -0.6 4,323,280 - Psyr_3640 0.71 -1.5 4,323,344 + -1.0 4,323,344 + +0.3 4,323,344 + +0.4 4,323,345 - -0.5 4,323,345 - -3.0 4,323,376 + -2.0 4,323,698 - +0.8 4,323,698 - -0.9 4,323,751 + +1.2 4,323,751 + +0.4 4,323,751 + +0.4 4,323,751 + -0.6 4,323,751 + +1.0 4,323,751 + +0.4 4,323,759 + -1.2 4,323,759 + +1.0 4,323,759 + +0.9 4,323,759 + -0.8 4,323,760 - -0.8 4,323,760 - -0.5 4,323,760 - +0.4 4,323,848 + -3.6 4,323,848 + -0.6 4,323,849 - -0.6 4,326,587 - +1.0
Or see this region's nucleotide sequence