Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3555 and Psyr_3556 are separated by 91 nucleotides Psyr_3556 and Psyr_3557 are separated by 69 nucleotides
Psyr_3555: Psyr_3555 - aspartate kinase, at 4,233,778 to 4,235,010
_3555
Psyr_3556: Psyr_3556 - alanyl-tRNA synthetase, at 4,235,102 to 4,237,801
_3556
Psyr_3557: Psyr_3557 - L-threonine aldolase, at 4,237,871 to 4,238,875
_3557
Position (kb)
4235
4236
4237
4238 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4234.291 kb on - strand, within Psyr_3555 at 4234.291 kb on - strand, within Psyr_3555 at 4234.291 kb on - strand, within Psyr_3555 at 4234.291 kb on - strand, within Psyr_3555 at 4235.048 kb on - strand at 4237.803 kb on - strand at 4237.803 kb on - strand at 4237.803 kb on - strand at 4237.803 kb on - strand at 4237.803 kb on - strand at 4237.805 kb on - strand at 4237.805 kb on - strand at 4237.805 kb on - strand at 4237.807 kb on - strand at 4237.853 kb on - strand at 4237.853 kb on - strand at 4237.853 kb on - strand at 4237.853 kb on - strand at 4237.974 kb on - strand, within Psyr_3557 at 4238.035 kb on - strand, within Psyr_3557 at 4238.035 kb on - strand, within Psyr_3557 at 4238.035 kb on - strand, within Psyr_3557 at 4238.035 kb on - strand, within Psyr_3557 at 4238.035 kb on - strand, within Psyr_3557 at 4238.152 kb on - strand, within Psyr_3557 at 4238.152 kb on - strand, within Psyr_3557 at 4238.359 kb on - strand, within Psyr_3557 at 4238.610 kb on + strand, within Psyr_3557 at 4238.610 kb on + strand, within Psyr_3557 at 4238.610 kb on + strand, within Psyr_3557 at 4238.610 kb on + strand, within Psyr_3557 at 4238.610 kb on + strand, within Psyr_3557 at 4238.610 kb on + strand, within Psyr_3557 at 4238.610 kb on + strand, within Psyr_3557 at 4238.611 kb on - strand, within Psyr_3557 at 4238.614 kb on + strand, within Psyr_3557 at 4238.614 kb on + strand, within Psyr_3557 at 4238.614 kb on + strand, within Psyr_3557 at 4238.614 kb on + strand, within Psyr_3557 at 4238.614 kb on + strand, within Psyr_3557 at 4238.614 kb on + strand, within Psyr_3557 at 4238.614 kb on + strand, within Psyr_3557 at 4238.615 kb on - strand, within Psyr_3557 at 4238.615 kb on - strand, within Psyr_3557 at 4238.615 kb on - strand, within Psyr_3557 at 4238.615 kb on - strand, within Psyr_3557 at 4238.615 kb on - strand, within Psyr_3557 at 4238.615 kb on - strand, within Psyr_3557 at 4238.615 kb on - strand, within Psyr_3557 at 4238.615 kb on - strand, within Psyr_3557 at 4238.616 kb on + strand, within Psyr_3557 at 4238.616 kb on + strand, within Psyr_3557 at 4238.616 kb on + strand, within Psyr_3557 at 4238.616 kb on + strand, within Psyr_3557 at 4238.616 kb on + strand, within Psyr_3557 at 4238.616 kb on + strand, within Psyr_3557 at 4238.616 kb on + strand, within Psyr_3557 at 4238.616 kb on + strand, within Psyr_3557 at 4238.616 kb on + strand, within Psyr_3557 at 4238.617 kb on - strand, within Psyr_3557 at 4238.617 kb on - strand, within Psyr_3557 at 4238.617 kb on - strand, within Psyr_3557 at 4238.617 kb on - strand, within Psyr_3557 at 4238.617 kb on - strand, within Psyr_3557 at 4238.617 kb on - strand, within Psyr_3557 at 4238.643 kb on + strand, within Psyr_3557 at 4238.643 kb on + strand, within Psyr_3557 at 4238.643 kb on + strand, within Psyr_3557 at 4238.644 kb on - strand, within Psyr_3557 at 4238.644 kb on - strand, within Psyr_3557 at 4238.644 kb on - strand, within Psyr_3557 at 4238.644 kb on - strand, within Psyr_3557 at 4238.644 kb on - strand, within Psyr_3557 at 4238.644 kb on - strand, within Psyr_3557 at 4238.644 kb on - strand, within Psyr_3557 at 4238.644 kb on - strand, within Psyr_3557 at 4238.644 kb on - strand, within Psyr_3557 at 4238.644 kb on - strand, within Psyr_3557
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 4,234,291 - Psyr_3555 0.42 +0.0 4,234,291 - Psyr_3555 0.42 -1.3 4,234,291 - Psyr_3555 0.42 +2.4 4,234,291 - Psyr_3555 0.42 +1.9 4,235,048 - +0.6 4,237,803 - +1.4 4,237,803 - -0.8 4,237,803 - -0.2 4,237,803 - -0.4 4,237,803 - +0.4 4,237,805 - +0.8 4,237,805 - -0.6 4,237,805 - +1.4 4,237,807 - +0.1 4,237,853 - +1.6 4,237,853 - -0.5 4,237,853 - +1.4 4,237,853 - -1.2 4,237,974 - Psyr_3557 0.10 +1.4 4,238,035 - Psyr_3557 0.16 +0.8 4,238,035 - Psyr_3557 0.16 +0.0 4,238,035 - Psyr_3557 0.16 -0.2 4,238,035 - Psyr_3557 0.16 -1.2 4,238,035 - Psyr_3557 0.16 +0.4 4,238,152 - Psyr_3557 0.28 -0.5 4,238,152 - Psyr_3557 0.28 +0.1 4,238,359 - Psyr_3557 0.49 +0.9 4,238,610 + Psyr_3557 0.74 -1.6 4,238,610 + Psyr_3557 0.74 +2.4 4,238,610 + Psyr_3557 0.74 -2.2 4,238,610 + Psyr_3557 0.74 +0.9 4,238,610 + Psyr_3557 0.74 -3.0 4,238,610 + Psyr_3557 0.74 +0.4 4,238,610 + Psyr_3557 0.74 -1.0 4,238,611 - Psyr_3557 0.74 +0.1 4,238,614 + Psyr_3557 0.74 +1.0 4,238,614 + Psyr_3557 0.74 -0.2 4,238,614 + Psyr_3557 0.74 -0.2 4,238,614 + Psyr_3557 0.74 +0.4 4,238,614 + Psyr_3557 0.74 -2.1 4,238,614 + Psyr_3557 0.74 -1.2 4,238,614 + Psyr_3557 0.74 +1.9 4,238,615 - Psyr_3557 0.74 -0.2 4,238,615 - Psyr_3557 0.74 +0.4 4,238,615 - Psyr_3557 0.74 +0.6 4,238,615 - Psyr_3557 0.74 -0.3 4,238,615 - Psyr_3557 0.74 -0.2 4,238,615 - Psyr_3557 0.74 +0.9 4,238,615 - Psyr_3557 0.74 -0.5 4,238,615 - Psyr_3557 0.74 +1.8 4,238,616 + Psyr_3557 0.74 +0.2 4,238,616 + Psyr_3557 0.74 +0.2 4,238,616 + Psyr_3557 0.74 +0.9 4,238,616 + Psyr_3557 0.74 -1.0 4,238,616 + Psyr_3557 0.74 +1.2 4,238,616 + Psyr_3557 0.74 +0.8 4,238,616 + Psyr_3557 0.74 +1.3 4,238,616 + Psyr_3557 0.74 +0.8 4,238,616 + Psyr_3557 0.74 -0.7 4,238,617 - Psyr_3557 0.74 +0.1 4,238,617 - Psyr_3557 0.74 +1.2 4,238,617 - Psyr_3557 0.74 -2.2 4,238,617 - Psyr_3557 0.74 -0.3 4,238,617 - Psyr_3557 0.74 -0.3 4,238,617 - Psyr_3557 0.74 +1.4 4,238,643 + Psyr_3557 0.77 -0.6 4,238,643 + Psyr_3557 0.77 +0.3 4,238,643 + Psyr_3557 0.77 +0.4 4,238,644 - Psyr_3557 0.77 -0.0 4,238,644 - Psyr_3557 0.77 +0.4 4,238,644 - Psyr_3557 0.77 +1.3 4,238,644 - Psyr_3557 0.77 +0.9 4,238,644 - Psyr_3557 0.77 +0.9 4,238,644 - Psyr_3557 0.77 +1.0 4,238,644 - Psyr_3557 0.77 -1.1 4,238,644 - Psyr_3557 0.77 -0.2 4,238,644 - Psyr_3557 0.77 -0.2 4,238,644 - Psyr_3557 0.77 +0.8
Or see this region's nucleotide sequence