Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3521 and Psyr_3522 are separated by 129 nucleotides Psyr_3522 and Psyr_3523 are separated by 140 nucleotides
Psyr_3521: Psyr_3521 - transcriptional regulator, LysR family, at 4,196,563 to 4,197,474
_3521
Psyr_3522: Psyr_3522 - L-serine ammonia-lyase, at 4,197,604 to 4,198,980
_3522
Psyr_3523: Psyr_3523 - Peptidase M20D, amidohydrolase, at 4,199,121 to 4,200,278
_3523
Position (kb)
4197
4198
4199 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4196.648 kb on + strand at 4196.648 kb on + strand at 4196.648 kb on + strand at 4196.840 kb on + strand, within Psyr_3521 at 4196.841 kb on - strand, within Psyr_3521 at 4196.864 kb on + strand, within Psyr_3521 at 4196.865 kb on - strand, within Psyr_3521 at 4196.865 kb on - strand, within Psyr_3521 at 4196.865 kb on - strand, within Psyr_3521 at 4197.206 kb on + strand, within Psyr_3521 at 4197.207 kb on - strand, within Psyr_3521 at 4197.500 kb on + strand at 4197.500 kb on + strand at 4197.500 kb on + strand at 4197.501 kb on - strand at 4197.546 kb on - strand at 4197.562 kb on + strand at 4197.609 kb on + strand at 4197.731 kb on + strand at 4197.731 kb on + strand at 4197.732 kb on - strand at 4197.732 kb on - strand at 4197.754 kb on + strand, within Psyr_3522 at 4197.754 kb on + strand, within Psyr_3522 at 4197.755 kb on - strand, within Psyr_3522 at 4197.864 kb on + strand, within Psyr_3522 at 4198.030 kb on + strand, within Psyr_3522 at 4198.030 kb on + strand, within Psyr_3522 at 4198.030 kb on + strand, within Psyr_3522 at 4198.030 kb on + strand, within Psyr_3522 at 4198.031 kb on - strand, within Psyr_3522 at 4198.031 kb on - strand, within Psyr_3522 at 4198.031 kb on - strand, within Psyr_3522 at 4198.031 kb on - strand, within Psyr_3522 at 4198.031 kb on - strand, within Psyr_3522 at 4198.115 kb on - strand, within Psyr_3522 at 4198.373 kb on + strand, within Psyr_3522 at 4198.374 kb on - strand, within Psyr_3522 at 4198.510 kb on + strand, within Psyr_3522 at 4198.510 kb on + strand, within Psyr_3522 at 4198.510 kb on + strand, within Psyr_3522 at 4198.511 kb on - strand, within Psyr_3522 at 4198.511 kb on - strand, within Psyr_3522 at 4198.511 kb on - strand, within Psyr_3522 at 4198.511 kb on - strand, within Psyr_3522 at 4198.511 kb on - strand, within Psyr_3522 at 4198.643 kb on + strand, within Psyr_3522 at 4198.644 kb on - strand, within Psyr_3522 at 4198.710 kb on - strand, within Psyr_3522 at 4198.740 kb on + strand, within Psyr_3522 at 4198.741 kb on - strand, within Psyr_3522 at 4198.930 kb on + strand at 4198.930 kb on + strand at 4198.930 kb on + strand at 4198.930 kb on + strand at 4198.931 kb on - strand at 4198.931 kb on - strand at 4198.931 kb on - strand at 4199.006 kb on - strand at 4199.027 kb on + strand at 4199.027 kb on + strand at 4199.027 kb on + strand at 4199.028 kb on - strand at 4199.028 kb on - strand at 4199.074 kb on + strand at 4199.074 kb on + strand at 4199.074 kb on + strand at 4199.082 kb on - strand at 4199.201 kb on - strand at 4199.203 kb on + strand at 4199.257 kb on + strand, within Psyr_3523 at 4199.257 kb on + strand, within Psyr_3523 at 4199.257 kb on + strand, within Psyr_3523 at 4199.257 kb on + strand, within Psyr_3523 at 4199.257 kb on + strand, within Psyr_3523 at 4199.257 kb on + strand, within Psyr_3523 at 4199.257 kb on + strand, within Psyr_3523 at 4199.257 kb on + strand, within Psyr_3523 at 4199.257 kb on + strand, within Psyr_3523 at 4199.258 kb on - strand, within Psyr_3523 at 4199.258 kb on - strand, within Psyr_3523 at 4199.258 kb on - strand, within Psyr_3523 at 4199.258 kb on - strand, within Psyr_3523 at 4199.258 kb on - strand, within Psyr_3523 at 4199.258 kb on - strand, within Psyr_3523 at 4199.258 kb on - strand, within Psyr_3523 at 4199.419 kb on + strand, within Psyr_3523 at 4199.420 kb on - strand, within Psyr_3523 at 4199.815 kb on + strand, within Psyr_3523 at 4199.816 kb on - strand, within Psyr_3523
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 4,196,648 + +0.8 4,196,648 + +0.3 4,196,648 + -0.1 4,196,840 + Psyr_3521 0.30 +0.1 4,196,841 - Psyr_3521 0.30 +1.4 4,196,864 + Psyr_3521 0.33 -0.0 4,196,865 - Psyr_3521 0.33 +0.6 4,196,865 - Psyr_3521 0.33 -0.7 4,196,865 - Psyr_3521 0.33 +0.5 4,197,206 + Psyr_3521 0.71 -1.7 4,197,207 - Psyr_3521 0.71 -0.8 4,197,500 + +0.1 4,197,500 + -1.1 4,197,500 + -0.2 4,197,501 - -0.7 4,197,546 - +1.0 4,197,562 + -1.7 4,197,609 + -1.8 4,197,731 + +1.6 4,197,731 + -0.2 4,197,732 - -1.3 4,197,732 - -1.5 4,197,754 + Psyr_3522 0.11 +0.6 4,197,754 + Psyr_3522 0.11 -2.5 4,197,755 - Psyr_3522 0.11 -2.4 4,197,864 + Psyr_3522 0.19 +0.0 4,198,030 + Psyr_3522 0.31 -1.4 4,198,030 + Psyr_3522 0.31 -0.4 4,198,030 + Psyr_3522 0.31 -0.7 4,198,030 + Psyr_3522 0.31 +1.2 4,198,031 - Psyr_3522 0.31 +0.7 4,198,031 - Psyr_3522 0.31 +0.9 4,198,031 - Psyr_3522 0.31 -2.1 4,198,031 - Psyr_3522 0.31 -1.0 4,198,031 - Psyr_3522 0.31 -2.0 4,198,115 - Psyr_3522 0.37 -0.7 4,198,373 + Psyr_3522 0.56 -0.4 4,198,374 - Psyr_3522 0.56 -0.2 4,198,510 + Psyr_3522 0.66 -1.0 4,198,510 + Psyr_3522 0.66 -1.3 4,198,510 + Psyr_3522 0.66 -0.7 4,198,511 - Psyr_3522 0.66 -1.2 4,198,511 - Psyr_3522 0.66 +0.8 4,198,511 - Psyr_3522 0.66 -0.6 4,198,511 - Psyr_3522 0.66 -1.1 4,198,511 - Psyr_3522 0.66 -0.1 4,198,643 + Psyr_3522 0.75 -2.7 4,198,644 - Psyr_3522 0.76 -1.7 4,198,710 - Psyr_3522 0.80 -0.5 4,198,740 + Psyr_3522 0.82 +0.3 4,198,741 - Psyr_3522 0.83 -0.2 4,198,930 + +0.5 4,198,930 + +0.0 4,198,930 + +0.3 4,198,930 + -3.0 4,198,931 - -0.2 4,198,931 - -2.2 4,198,931 - -1.0 4,199,006 - -0.1 4,199,027 + +1.0 4,199,027 + +0.8 4,199,027 + -0.7 4,199,028 - -0.3 4,199,028 - +0.7 4,199,074 + +1.0 4,199,074 + +0.1 4,199,074 + +0.3 4,199,082 - +0.8 4,199,201 - +0.5 4,199,203 + -0.7 4,199,257 + Psyr_3523 0.12 -0.5 4,199,257 + Psyr_3523 0.12 +0.0 4,199,257 + Psyr_3523 0.12 +0.7 4,199,257 + Psyr_3523 0.12 -0.5 4,199,257 + Psyr_3523 0.12 +1.3 4,199,257 + Psyr_3523 0.12 -1.0 4,199,257 + Psyr_3523 0.12 -1.0 4,199,257 + Psyr_3523 0.12 -2.4 4,199,257 + Psyr_3523 0.12 -0.7 4,199,258 - Psyr_3523 0.12 +0.2 4,199,258 - Psyr_3523 0.12 -0.4 4,199,258 - Psyr_3523 0.12 +0.9 4,199,258 - Psyr_3523 0.12 +2.4 4,199,258 - Psyr_3523 0.12 +0.2 4,199,258 - Psyr_3523 0.12 +0.8 4,199,258 - Psyr_3523 0.12 +1.1 4,199,419 + Psyr_3523 0.26 -2.1 4,199,420 - Psyr_3523 0.26 +0.3 4,199,815 + Psyr_3523 0.60 -1.8 4,199,816 - Psyr_3523 0.60 +1.2
Or see this region's nucleotide sequence