Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3370 and Psyr_3371 are separated by 565 nucleotides Psyr_3371 and Psyr_3372 overlap by 4 nucleotides Psyr_3372 and Psyr_3373 are separated by 74 nucleotides
Psyr_3370: Psyr_3370 - Protein of unknown function DUF451, at 4,029,281 to 4,030,138
_3370
Psyr_3371: Psyr_3371 - Oxidoreductase, molybdopterin binding protein, at 4,030,704 to 4,031,474
_3371
Psyr_3372: Psyr_3372 - conserved hypothetical protein, at 4,031,471 to 4,032,091
_3372
Psyr_3373: Psyr_3373 - conserved hypothetical protein, at 4,032,166 to 4,032,771
_3373
Position (kb)
4030
4031
4032 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4029.872 kb on + strand, within Psyr_3370 at 4029.873 kb on - strand, within Psyr_3370 at 4030.007 kb on + strand, within Psyr_3370 at 4030.007 kb on + strand, within Psyr_3370 at 4030.007 kb on + strand, within Psyr_3370 at 4030.008 kb on - strand, within Psyr_3370 at 4030.008 kb on - strand, within Psyr_3370 at 4030.008 kb on - strand, within Psyr_3370 at 4030.008 kb on - strand, within Psyr_3370 at 4030.008 kb on - strand, within Psyr_3370 at 4030.008 kb on - strand, within Psyr_3370 at 4030.008 kb on - strand, within Psyr_3370 at 4030.191 kb on - strand at 4030.254 kb on + strand at 4030.273 kb on + strand at 4030.282 kb on - strand at 4030.510 kb on - strand at 4030.705 kb on + strand at 4030.706 kb on - strand at 4030.726 kb on + strand at 4030.727 kb on - strand at 4030.727 kb on - strand at 4030.727 kb on - strand at 4030.764 kb on - strand at 4030.907 kb on + strand, within Psyr_3371 at 4030.927 kb on + strand, within Psyr_3371 at 4030.928 kb on - strand, within Psyr_3371 at 4030.928 kb on - strand, within Psyr_3371 at 4030.984 kb on + strand, within Psyr_3371 at 4030.984 kb on + strand, within Psyr_3371 at 4030.984 kb on + strand, within Psyr_3371 at 4030.984 kb on + strand, within Psyr_3371 at 4030.985 kb on - strand, within Psyr_3371 at 4031.176 kb on + strand, within Psyr_3371 at 4031.176 kb on + strand, within Psyr_3371 at 4031.176 kb on + strand, within Psyr_3371 at 4031.176 kb on + strand, within Psyr_3371 at 4031.176 kb on + strand, within Psyr_3371 at 4031.177 kb on - strand, within Psyr_3371 at 4031.177 kb on - strand, within Psyr_3371 at 4031.177 kb on - strand, within Psyr_3371 at 4031.177 kb on - strand, within Psyr_3371 at 4031.228 kb on + strand, within Psyr_3371 at 4031.230 kb on + strand, within Psyr_3371 at 4031.230 kb on + strand, within Psyr_3371 at 4031.230 kb on + strand, within Psyr_3371 at 4031.231 kb on - strand, within Psyr_3371 at 4031.231 kb on - strand, within Psyr_3371 at 4031.231 kb on - strand, within Psyr_3371 at 4031.231 kb on - strand, within Psyr_3371 at 4031.453 kb on + strand at 4031.453 kb on + strand at 4031.459 kb on + strand at 4031.533 kb on + strand, within Psyr_3372 at 4031.534 kb on - strand, within Psyr_3372 at 4031.607 kb on - strand, within Psyr_3372 at 4031.699 kb on + strand, within Psyr_3372 at 4031.724 kb on + strand, within Psyr_3372 at 4031.724 kb on + strand, within Psyr_3372 at 4031.724 kb on + strand, within Psyr_3372 at 4031.724 kb on + strand, within Psyr_3372 at 4031.724 kb on + strand, within Psyr_3372 at 4031.724 kb on + strand, within Psyr_3372 at 4031.725 kb on - strand, within Psyr_3372 at 4031.725 kb on - strand, within Psyr_3372 at 4031.725 kb on - strand, within Psyr_3372 at 4031.725 kb on - strand, within Psyr_3372 at 4031.725 kb on - strand, within Psyr_3372 at 4031.725 kb on - strand, within Psyr_3372 at 4031.871 kb on + strand, within Psyr_3372 at 4031.872 kb on - strand, within Psyr_3372 at 4031.872 kb on - strand, within Psyr_3372 at 4031.872 kb on - strand, within Psyr_3372 at 4031.994 kb on + strand, within Psyr_3372 at 4032.019 kb on + strand, within Psyr_3372 at 4032.019 kb on + strand, within Psyr_3372 at 4032.019 kb on + strand, within Psyr_3372 at 4032.019 kb on + strand, within Psyr_3372 at 4032.019 kb on + strand, within Psyr_3372 at 4032.019 kb on + strand, within Psyr_3372 at 4032.019 kb on + strand, within Psyr_3372 at 4032.020 kb on - strand, within Psyr_3372 at 4032.020 kb on - strand, within Psyr_3372 at 4032.020 kb on - strand, within Psyr_3372 at 4032.020 kb on - strand, within Psyr_3372 at 4032.020 kb on - strand, within Psyr_3372 at 4032.167 kb on + strand at 4032.167 kb on + strand at 4032.167 kb on + strand at 4032.167 kb on + strand at 4032.168 kb on - strand at 4032.168 kb on - strand at 4032.168 kb on - strand at 4032.168 kb on - strand at 4032.238 kb on + strand, within Psyr_3373 at 4032.341 kb on + strand, within Psyr_3373 at 4032.341 kb on + strand, within Psyr_3373 at 4032.341 kb on + strand, within Psyr_3373 at 4032.341 kb on + strand, within Psyr_3373 at 4032.341 kb on + strand, within Psyr_3373 at 4032.342 kb on - strand, within Psyr_3373 at 4032.342 kb on - strand, within Psyr_3373 at 4032.342 kb on - strand, within Psyr_3373 at 4032.342 kb on - strand, within Psyr_3373 at 4032.342 kb on - strand, within Psyr_3373
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 4,029,872 + Psyr_3370 0.69 -0.0 4,029,873 - Psyr_3370 0.69 -0.2 4,030,007 + Psyr_3370 0.85 +0.9 4,030,007 + Psyr_3370 0.85 +1.1 4,030,007 + Psyr_3370 0.85 -0.7 4,030,008 - Psyr_3370 0.85 -0.3 4,030,008 - Psyr_3370 0.85 -1.0 4,030,008 - Psyr_3370 0.85 +0.0 4,030,008 - Psyr_3370 0.85 -0.2 4,030,008 - Psyr_3370 0.85 +0.0 4,030,008 - Psyr_3370 0.85 -0.3 4,030,008 - Psyr_3370 0.85 +0.1 4,030,191 - -0.3 4,030,254 + -0.1 4,030,273 + +0.0 4,030,282 - +3.7 4,030,510 - -0.9 4,030,705 + -0.5 4,030,706 - +0.2 4,030,726 + -0.3 4,030,727 - +0.8 4,030,727 - +0.9 4,030,727 - +0.4 4,030,764 - +0.4 4,030,907 + Psyr_3371 0.26 -0.2 4,030,927 + Psyr_3371 0.29 -1.1 4,030,928 - Psyr_3371 0.29 -0.8 4,030,928 - Psyr_3371 0.29 +0.2 4,030,984 + Psyr_3371 0.36 -0.3 4,030,984 + Psyr_3371 0.36 +1.1 4,030,984 + Psyr_3371 0.36 -0.6 4,030,984 + Psyr_3371 0.36 -0.7 4,030,985 - Psyr_3371 0.36 -0.4 4,031,176 + Psyr_3371 0.61 -1.0 4,031,176 + Psyr_3371 0.61 -1.7 4,031,176 + Psyr_3371 0.61 -2.1 4,031,176 + Psyr_3371 0.61 -1.4 4,031,176 + Psyr_3371 0.61 +0.9 4,031,177 - Psyr_3371 0.61 -0.5 4,031,177 - Psyr_3371 0.61 +0.4 4,031,177 - Psyr_3371 0.61 +0.3 4,031,177 - Psyr_3371 0.61 +0.2 4,031,228 + Psyr_3371 0.68 -0.5 4,031,230 + Psyr_3371 0.68 -2.3 4,031,230 + Psyr_3371 0.68 -0.3 4,031,230 + Psyr_3371 0.68 -0.3 4,031,231 - Psyr_3371 0.68 +0.2 4,031,231 - Psyr_3371 0.68 +0.7 4,031,231 - Psyr_3371 0.68 -0.5 4,031,231 - Psyr_3371 0.68 -0.5 4,031,453 + -0.5 4,031,453 + -1.3 4,031,459 + +1.3 4,031,533 + Psyr_3372 0.10 -2.1 4,031,534 - Psyr_3372 0.10 +1.5 4,031,607 - Psyr_3372 0.22 +1.3 4,031,699 + Psyr_3372 0.37 +0.1 4,031,724 + Psyr_3372 0.41 +0.7 4,031,724 + Psyr_3372 0.41 +0.5 4,031,724 + Psyr_3372 0.41 +0.5 4,031,724 + Psyr_3372 0.41 +0.1 4,031,724 + Psyr_3372 0.41 -0.3 4,031,724 + Psyr_3372 0.41 -1.6 4,031,725 - Psyr_3372 0.41 +1.4 4,031,725 - Psyr_3372 0.41 +0.3 4,031,725 - Psyr_3372 0.41 +1.2 4,031,725 - Psyr_3372 0.41 -0.1 4,031,725 - Psyr_3372 0.41 -0.5 4,031,725 - Psyr_3372 0.41 +0.4 4,031,871 + Psyr_3372 0.64 -0.0 4,031,872 - Psyr_3372 0.65 -0.6 4,031,872 - Psyr_3372 0.65 +0.8 4,031,872 - Psyr_3372 0.65 +1.1 4,031,994 + Psyr_3372 0.84 -0.5 4,032,019 + Psyr_3372 0.88 -0.3 4,032,019 + Psyr_3372 0.88 -0.5 4,032,019 + Psyr_3372 0.88 +0.6 4,032,019 + Psyr_3372 0.88 -0.4 4,032,019 + Psyr_3372 0.88 +1.3 4,032,019 + Psyr_3372 0.88 -1.6 4,032,019 + Psyr_3372 0.88 -0.1 4,032,020 - Psyr_3372 0.88 +0.1 4,032,020 - Psyr_3372 0.88 -1.0 4,032,020 - Psyr_3372 0.88 +0.7 4,032,020 - Psyr_3372 0.88 +0.0 4,032,020 - Psyr_3372 0.88 +1.6 4,032,167 + -0.3 4,032,167 + +0.1 4,032,167 + +0.0 4,032,167 + -0.1 4,032,168 - +0.6 4,032,168 - +0.3 4,032,168 - +0.0 4,032,168 - +0.5 4,032,238 + Psyr_3373 0.12 -1.4 4,032,341 + Psyr_3373 0.29 -2.0 4,032,341 + Psyr_3373 0.29 +1.3 4,032,341 + Psyr_3373 0.29 -0.7 4,032,341 + Psyr_3373 0.29 -0.5 4,032,341 + Psyr_3373 0.29 -1.4 4,032,342 - Psyr_3373 0.29 +0.6 4,032,342 - Psyr_3373 0.29 +0.2 4,032,342 - Psyr_3373 0.29 -0.1 4,032,342 - Psyr_3373 0.29 +1.0 4,032,342 - Psyr_3373 0.29 +0.7
Or see this region's nucleotide sequence