Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3204 and Psyr_3205 are separated by 10 nucleotides Psyr_3205 and Psyr_3206 overlap by 4 nucleotides Psyr_3206 and Psyr_3207 overlap by 4 nucleotides Psyr_3207 and Psyr_3208 are separated by 39 nucleotides
Psyr_3204: Psyr_3204 - NADH dehydrogenase subunit I, at 3,836,385 to 3,836,933
_3204
Psyr_3205: Psyr_3205 - NADH dehydrogenase subunit J, at 3,836,944 to 3,837,453
_3205
Psyr_3206: Psyr_3206 - NADH dehydrogenase subunit K, at 3,837,450 to 3,837,758
_3206
Psyr_3207: Psyr_3207 - NADH dehydrogenase subunit L, at 3,837,755 to 3,839,608
_3207
Psyr_3208: Psyr_3208 - NADH dehydrogenase subunit M, at 3,839,648 to 3,841,180
_3208
Position (kb) 3837
3838
3839
3840 Strain fitness (log2 ratio) -1
0
1
2 at 3836.785 kb on - strand, within Psyr_3204 at 3837.268 kb on + strand, within Psyr_3205 at 3837.915 kb on + strand at 3837.916 kb on - strand at 3837.998 kb on + strand, within Psyr_3207 at 3838.076 kb on + strand, within Psyr_3207 at 3838.198 kb on - strand, within Psyr_3207 at 3839.043 kb on - strand, within Psyr_3207 at 3839.439 kb on + strand at 3840.315 kb on - strand, within Psyr_3208
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 3,836,785 - Psyr_3204 0.73 -0.5 3,837,268 + Psyr_3205 0.64 -0.2 3,837,915 + +1.2 3,837,916 - -0.9 3,837,998 + Psyr_3207 0.13 -0.3 3,838,076 + Psyr_3207 0.17 -1.0 3,838,198 - Psyr_3207 0.24 +0.3 3,839,043 - Psyr_3207 0.69 -1.7 3,839,439 + +1.9 3,840,315 - Psyr_3208 0.44 +1.3
Or see this region's nucleotide sequence