Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2655 and Psyr_2656 are separated by 171 nucleotides Psyr_2656 and Psyr_2657 are separated by 143 nucleotides Psyr_2657 and Psyr_2658 are separated by 197 nucleotides
Psyr_2655: Psyr_2655 - Nitroreductase, at 3,225,794 to 3,226,615
_2655
Psyr_2656: Psyr_2656 - hypothetical protein, at 3,226,787 to 3,227,137
_2656
Psyr_2657: Psyr_2657 - hypothetical protein, at 3,227,281 to 3,228,120
_2657
Psyr_2658: Psyr_2658 - hypothetical protein, at 3,228,318 to 3,229,451
_2658
Position (kb) 3227
3228
3229 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 3226.347 kb on + strand, within Psyr_2655 at 3226.425 kb on - strand, within Psyr_2655 at 3226.459 kb on - strand, within Psyr_2655 at 3226.459 kb on - strand, within Psyr_2655 at 3226.460 kb on + strand, within Psyr_2655 at 3226.460 kb on + strand, within Psyr_2655 at 3226.460 kb on + strand, within Psyr_2655 at 3226.460 kb on + strand, within Psyr_2655 at 3226.461 kb on - strand, within Psyr_2655 at 3226.461 kb on - strand, within Psyr_2655 at 3226.701 kb on + strand at 3226.701 kb on + strand at 3226.701 kb on + strand at 3226.701 kb on + strand at 3226.701 kb on + strand at 3226.701 kb on + strand at 3226.701 kb on + strand at 3226.701 kb on + strand at 3226.702 kb on - strand at 3226.702 kb on - strand at 3226.702 kb on - strand at 3226.702 kb on - strand at 3226.702 kb on - strand at 3226.727 kb on - strand at 3226.727 kb on - strand at 3226.757 kb on + strand at 3226.758 kb on - strand at 3226.758 kb on - strand at 3226.768 kb on - strand at 3226.771 kb on + strand at 3226.771 kb on + strand at 3226.771 kb on + strand at 3226.772 kb on - strand at 3226.772 kb on - strand at 3226.772 kb on - strand at 3226.772 kb on - strand at 3226.772 kb on - strand at 3226.772 kb on - strand at 3226.788 kb on + strand at 3226.789 kb on - strand at 3226.841 kb on - strand, within Psyr_2656 at 3226.841 kb on - strand, within Psyr_2656 at 3226.863 kb on + strand, within Psyr_2656 at 3226.938 kb on + strand, within Psyr_2656 at 3226.938 kb on + strand, within Psyr_2656 at 3226.939 kb on - strand, within Psyr_2656 at 3226.942 kb on + strand, within Psyr_2656 at 3226.942 kb on + strand, within Psyr_2656 at 3226.942 kb on + strand, within Psyr_2656 at 3226.942 kb on + strand, within Psyr_2656 at 3226.942 kb on + strand, within Psyr_2656 at 3226.942 kb on + strand, within Psyr_2656 at 3226.943 kb on - strand, within Psyr_2656 at 3226.943 kb on - strand, within Psyr_2656 at 3226.943 kb on - strand, within Psyr_2656 at 3226.943 kb on - strand, within Psyr_2656 at 3226.943 kb on - strand, within Psyr_2656 at 3226.943 kb on - strand, within Psyr_2656 at 3226.946 kb on + strand, within Psyr_2656 at 3226.946 kb on + strand, within Psyr_2656 at 3226.946 kb on + strand, within Psyr_2656 at 3226.946 kb on + strand, within Psyr_2656 at 3226.947 kb on - strand, within Psyr_2656 at 3226.947 kb on - strand, within Psyr_2656 at 3227.060 kb on + strand, within Psyr_2656 at 3227.061 kb on - strand, within Psyr_2656 at 3227.061 kb on - strand, within Psyr_2656 at 3227.149 kb on + strand at 3227.149 kb on + strand at 3227.149 kb on + strand at 3227.149 kb on + strand at 3227.149 kb on + strand at 3227.150 kb on - strand at 3227.150 kb on - strand at 3227.150 kb on - strand at 3227.150 kb on - strand at 3227.150 kb on - strand at 3227.150 kb on - strand at 3227.150 kb on - strand at 3227.204 kb on + strand at 3227.204 kb on + strand at 3227.205 kb on - strand at 3227.205 kb on - strand at 3227.205 kb on - strand at 3227.306 kb on - strand at 3227.306 kb on - strand at 3227.306 kb on - strand at 3227.377 kb on + strand, within Psyr_2657 at 3227.377 kb on + strand at 3227.378 kb on - strand, within Psyr_2657 at 3227.378 kb on - strand, within Psyr_2657 at 3227.378 kb on - strand, within Psyr_2657 at 3227.378 kb on - strand, within Psyr_2657 at 3227.378 kb on - strand, within Psyr_2657 at 3227.378 kb on - strand, within Psyr_2657 at 3227.421 kb on - strand, within Psyr_2657 at 3227.451 kb on + strand, within Psyr_2657 at 3227.452 kb on - strand, within Psyr_2657 at 3227.503 kb on + strand, within Psyr_2657 at 3227.504 kb on - strand, within Psyr_2657 at 3227.617 kb on + strand, within Psyr_2657 at 3227.617 kb on + strand, within Psyr_2657 at 3227.617 kb on + strand, within Psyr_2657 at 3227.618 kb on - strand, within Psyr_2657 at 3227.618 kb on - strand, within Psyr_2657 at 3227.618 kb on - strand, within Psyr_2657 at 3227.705 kb on + strand, within Psyr_2657 at 3227.706 kb on - strand, within Psyr_2657 at 3227.803 kb on + strand, within Psyr_2657 at 3227.803 kb on + strand, within Psyr_2657 at 3227.817 kb on + strand, within Psyr_2657 at 3227.817 kb on + strand, within Psyr_2657 at 3227.817 kb on + strand, within Psyr_2657 at 3227.817 kb on + strand, within Psyr_2657 at 3227.817 kb on + strand, within Psyr_2657 at 3227.817 kb on + strand, within Psyr_2657 at 3227.817 kb on + strand, within Psyr_2657 at 3227.817 kb on + strand, within Psyr_2657 at 3227.817 kb on + strand, within Psyr_2657 at 3227.818 kb on - strand, within Psyr_2657 at 3227.818 kb on - strand, within Psyr_2657 at 3227.818 kb on - strand, within Psyr_2657 at 3227.818 kb on - strand, within Psyr_2657 at 3227.818 kb on - strand, within Psyr_2657 at 3227.818 kb on - strand, within Psyr_2657 at 3227.818 kb on - strand, within Psyr_2657 at 3227.829 kb on + strand, within Psyr_2657 at 3227.830 kb on - strand, within Psyr_2657 at 3227.830 kb on - strand, within Psyr_2657 at 3227.872 kb on - strand, within Psyr_2657 at 3227.872 kb on - strand, within Psyr_2657 at 3227.899 kb on - strand, within Psyr_2657 at 3228.013 kb on + strand, within Psyr_2657 at 3228.013 kb on + strand, within Psyr_2657 at 3228.027 kb on + strand, within Psyr_2657 at 3228.027 kb on + strand, within Psyr_2657 at 3228.028 kb on - strand, within Psyr_2657 at 3228.028 kb on - strand, within Psyr_2657 at 3228.063 kb on + strand at 3228.064 kb on - strand at 3228.160 kb on + strand at 3228.160 kb on + strand at 3228.161 kb on - strand at 3228.161 kb on - strand at 3228.223 kb on - strand at 3228.223 kb on - strand at 3228.232 kb on + strand at 3228.232 kb on + strand at 3228.232 kb on + strand at 3228.232 kb on + strand at 3228.232 kb on + strand at 3228.233 kb on - strand at 3228.233 kb on - strand at 3228.233 kb on - strand at 3228.233 kb on - strand at 3228.233 kb on - strand at 3228.233 kb on - strand at 3228.233 kb on - strand at 3228.233 kb on - strand at 3228.268 kb on + strand at 3228.268 kb on + strand at 3228.268 kb on + strand at 3228.268 kb on + strand at 3228.268 kb on + strand at 3228.268 kb on + strand at 3228.268 kb on + strand at 3228.269 kb on - strand at 3228.269 kb on - strand at 3228.269 kb on - strand at 3228.269 kb on - strand at 3228.269 kb on - strand at 3228.269 kb on - strand at 3228.269 kb on - strand at 3228.269 kb on - strand at 3228.269 kb on - strand at 3228.269 kb on - strand at 3228.304 kb on + strand at 3228.304 kb on + strand at 3228.304 kb on + strand at 3228.304 kb on + strand at 3228.304 kb on + strand at 3228.305 kb on - strand at 3228.305 kb on - strand at 3228.305 kb on - strand at 3228.305 kb on - strand at 3228.308 kb on + strand at 3228.368 kb on - strand at 3228.368 kb on - strand at 3228.405 kb on - strand at 3228.405 kb on - strand at 3228.405 kb on - strand at 3228.418 kb on + strand at 3228.418 kb on + strand at 3228.418 kb on + strand at 3228.418 kb on + strand at 3228.418 kb on + strand at 3228.419 kb on - strand at 3228.419 kb on - strand at 3228.421 kb on + strand at 3228.421 kb on + strand at 3228.421 kb on + strand at 3228.421 kb on + strand at 3228.421 kb on + strand at 3228.421 kb on + strand at 3228.422 kb on - strand at 3228.553 kb on + strand, within Psyr_2658 at 3228.554 kb on - strand, within Psyr_2658 at 3228.554 kb on - strand, within Psyr_2658 at 3228.584 kb on + strand, within Psyr_2658 at 3228.613 kb on + strand, within Psyr_2658 at 3228.830 kb on - strand, within Psyr_2658 at 3228.830 kb on - strand, within Psyr_2658 at 3228.873 kb on - strand, within Psyr_2658 at 3228.881 kb on - strand, within Psyr_2658 at 3228.976 kb on + strand, within Psyr_2658 at 3228.976 kb on + strand, within Psyr_2658 at 3228.976 kb on + strand, within Psyr_2658 at 3228.976 kb on + strand, within Psyr_2658 at 3228.976 kb on + strand, within Psyr_2658 at 3228.976 kb on + strand, within Psyr_2658 at 3228.976 kb on + strand, within Psyr_2658 at 3228.976 kb on + strand, within Psyr_2658 at 3228.976 kb on + strand, within Psyr_2658 at 3228.976 kb on + strand, within Psyr_2658 at 3228.977 kb on - strand, within Psyr_2658 at 3228.977 kb on - strand, within Psyr_2658 at 3228.977 kb on - strand, within Psyr_2658 at 3228.998 kb on + strand, within Psyr_2658 at 3228.999 kb on - strand, within Psyr_2658 at 3229.027 kb on + strand, within Psyr_2658 at 3229.028 kb on - strand, within Psyr_2658 at 3229.028 kb on - strand, within Psyr_2658 at 3229.028 kb on - strand, within Psyr_2658 at 3229.089 kb on + strand, within Psyr_2658
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 3,226,347 + Psyr_2655 0.67 -1.7 3,226,425 - Psyr_2655 0.77 +0.0 3,226,459 - Psyr_2655 0.81 +0.3 3,226,459 - Psyr_2655 0.81 +0.2 3,226,460 + Psyr_2655 0.81 -0.9 3,226,460 + Psyr_2655 0.81 -0.7 3,226,460 + Psyr_2655 0.81 +0.1 3,226,460 + Psyr_2655 0.81 +0.8 3,226,461 - Psyr_2655 0.81 +0.3 3,226,461 - Psyr_2655 0.81 -0.5 3,226,701 + -0.0 3,226,701 + +0.3 3,226,701 + -1.8 3,226,701 + -0.0 3,226,701 + -0.2 3,226,701 + +0.2 3,226,701 + -1.1 3,226,701 + +0.6 3,226,702 - +0.2 3,226,702 - +0.3 3,226,702 - +0.1 3,226,702 - -0.5 3,226,702 - -0.1 3,226,727 - +2.1 3,226,727 - +3.3 3,226,757 + -2.3 3,226,758 - +0.7 3,226,758 - +1.1 3,226,768 - -1.0 3,226,771 + +0.8 3,226,771 + -0.9 3,226,771 + +1.1 3,226,772 - -0.9 3,226,772 - -0.5 3,226,772 - -1.1 3,226,772 - +2.5 3,226,772 - -0.9 3,226,772 - -0.1 3,226,788 + +0.3 3,226,789 - +0.4 3,226,841 - Psyr_2656 0.15 +0.8 3,226,841 - Psyr_2656 0.15 -0.1 3,226,863 + Psyr_2656 0.22 -0.6 3,226,938 + Psyr_2656 0.43 -2.0 3,226,938 + Psyr_2656 0.43 +0.0 3,226,939 - Psyr_2656 0.43 -0.1 3,226,942 + Psyr_2656 0.44 -0.5 3,226,942 + Psyr_2656 0.44 +0.5 3,226,942 + Psyr_2656 0.44 -0.1 3,226,942 + Psyr_2656 0.44 +0.5 3,226,942 + Psyr_2656 0.44 +0.5 3,226,942 + Psyr_2656 0.44 -2.4 3,226,943 - Psyr_2656 0.44 +1.0 3,226,943 - Psyr_2656 0.44 +1.2 3,226,943 - Psyr_2656 0.44 +1.3 3,226,943 - Psyr_2656 0.44 -0.7 3,226,943 - Psyr_2656 0.44 +0.6 3,226,943 - Psyr_2656 0.44 +1.8 3,226,946 + Psyr_2656 0.45 -1.3 3,226,946 + Psyr_2656 0.45 +0.1 3,226,946 + Psyr_2656 0.45 +0.1 3,226,946 + Psyr_2656 0.45 -1.1 3,226,947 - Psyr_2656 0.46 -0.2 3,226,947 - Psyr_2656 0.46 +0.2 3,227,060 + Psyr_2656 0.78 -1.3 3,227,061 - Psyr_2656 0.78 -0.4 3,227,061 - Psyr_2656 0.78 +1.5 3,227,149 + +0.1 3,227,149 + -0.7 3,227,149 + +0.5 3,227,149 + -0.0 3,227,149 + +0.4 3,227,150 - +1.8 3,227,150 - -0.5 3,227,150 - -1.7 3,227,150 - -0.1 3,227,150 - -0.3 3,227,150 - -0.1 3,227,150 - -2.7 3,227,204 + +0.2 3,227,204 + -0.4 3,227,205 - +0.5 3,227,205 - +1.5 3,227,205 - -0.0 3,227,306 - +0.3 3,227,306 - +0.9 3,227,306 - +0.3 3,227,377 + Psyr_2657 0.11 +0.9 3,227,377 + +1.9 3,227,378 - Psyr_2657 0.12 +0.3 3,227,378 - Psyr_2657 0.12 -1.7 3,227,378 - Psyr_2657 0.12 +0.9 3,227,378 - Psyr_2657 0.12 -0.5 3,227,378 - Psyr_2657 0.12 -0.7 3,227,378 - Psyr_2657 0.12 -2.6 3,227,421 - Psyr_2657 0.17 -0.0 3,227,451 + Psyr_2657 0.20 -1.1 3,227,452 - Psyr_2657 0.20 -0.7 3,227,503 + Psyr_2657 0.26 +0.5 3,227,504 - Psyr_2657 0.27 +0.1 3,227,617 + Psyr_2657 0.40 -0.1 3,227,617 + Psyr_2657 0.40 -1.2 3,227,617 + Psyr_2657 0.40 -1.0 3,227,618 - Psyr_2657 0.40 -1.2 3,227,618 - Psyr_2657 0.40 +0.1 3,227,618 - Psyr_2657 0.40 -0.0 3,227,705 + Psyr_2657 0.50 -0.7 3,227,706 - Psyr_2657 0.51 +0.1 3,227,803 + Psyr_2657 0.62 -0.9 3,227,803 + Psyr_2657 0.62 +0.5 3,227,817 + Psyr_2657 0.64 -0.9 3,227,817 + Psyr_2657 0.64 -1.7 3,227,817 + Psyr_2657 0.64 +0.1 3,227,817 + Psyr_2657 0.64 +0.0 3,227,817 + Psyr_2657 0.64 -0.4 3,227,817 + Psyr_2657 0.64 -1.9 3,227,817 + Psyr_2657 0.64 +0.1 3,227,817 + Psyr_2657 0.64 +0.7 3,227,817 + Psyr_2657 0.64 -1.3 3,227,818 - Psyr_2657 0.64 +0.5 3,227,818 - Psyr_2657 0.64 -1.5 3,227,818 - Psyr_2657 0.64 -0.1 3,227,818 - Psyr_2657 0.64 +1.5 3,227,818 - Psyr_2657 0.64 +1.5 3,227,818 - Psyr_2657 0.64 -1.1 3,227,818 - Psyr_2657 0.64 -1.7 3,227,829 + Psyr_2657 0.65 +0.0 3,227,830 - Psyr_2657 0.65 +0.5 3,227,830 - Psyr_2657 0.65 -1.3 3,227,872 - Psyr_2657 0.70 +2.2 3,227,872 - Psyr_2657 0.70 -1.3 3,227,899 - Psyr_2657 0.74 -0.1 3,228,013 + Psyr_2657 0.87 -2.1 3,228,013 + Psyr_2657 0.87 -0.5 3,228,027 + Psyr_2657 0.89 -0.7 3,228,027 + Psyr_2657 0.89 -1.3 3,228,028 - Psyr_2657 0.89 -0.1 3,228,028 - Psyr_2657 0.89 +1.5 3,228,063 + -2.5 3,228,064 - -2.5 3,228,160 + -0.1 3,228,160 + -0.3 3,228,161 - -0.5 3,228,161 - +0.5 3,228,223 - +0.1 3,228,223 - -0.9 3,228,232 + -0.5 3,228,232 + +0.5 3,228,232 + -0.5 3,228,232 + +0.5 3,228,232 + +2.1 3,228,233 - -0.9 3,228,233 - -0.0 3,228,233 - -1.3 3,228,233 - -0.5 3,228,233 - -0.5 3,228,233 - +1.5 3,228,233 - -1.0 3,228,233 - -1.3 3,228,268 + +1.4 3,228,268 + +0.5 3,228,268 + +0.9 3,228,268 + -0.1 3,228,268 + +0.5 3,228,268 + -0.9 3,228,268 + +0.5 3,228,269 - -2.0 3,228,269 - +0.2 3,228,269 - +0.7 3,228,269 - +1.5 3,228,269 - -1.2 3,228,269 - -1.9 3,228,269 - -1.5 3,228,269 - -1.7 3,228,269 - -1.4 3,228,269 - +1.5 3,228,304 + -0.5 3,228,304 + +1.5 3,228,304 + -0.1 3,228,304 + -2.0 3,228,304 + -1.2 3,228,305 - -0.9 3,228,305 - -0.5 3,228,305 - -0.1 3,228,305 - -0.5 3,228,308 + -1.5 3,228,368 - +0.5 3,228,368 - +1.6 3,228,405 - -0.7 3,228,405 - +1.5 3,228,405 - -0.9 3,228,418 + +0.5 3,228,418 + -2.9 3,228,418 + -0.9 3,228,418 + -0.1 3,228,418 + +1.5 3,228,419 - -0.5 3,228,419 - -0.5 3,228,421 + -1.9 3,228,421 + +0.1 3,228,421 + -0.5 3,228,421 + +1.5 3,228,421 + -0.5 3,228,421 + +1.5 3,228,422 - -2.5 3,228,553 + Psyr_2658 0.21 +0.5 3,228,554 - Psyr_2658 0.21 -2.9 3,228,554 - Psyr_2658 0.21 +0.5 3,228,584 + Psyr_2658 0.23 +1.5 3,228,613 + Psyr_2658 0.26 +0.5 3,228,830 - Psyr_2658 0.45 -0.5 3,228,830 - Psyr_2658 0.45 -1.6 3,228,873 - Psyr_2658 0.49 -0.9 3,228,881 - Psyr_2658 0.50 -2.5 3,228,976 + Psyr_2658 0.58 -1.5 3,228,976 + Psyr_2658 0.58 -0.2 3,228,976 + Psyr_2658 0.58 +1.1 3,228,976 + Psyr_2658 0.58 -0.7 3,228,976 + Psyr_2658 0.58 -0.2 3,228,976 + Psyr_2658 0.58 +0.9 3,228,976 + Psyr_2658 0.58 +0.1 3,228,976 + Psyr_2658 0.58 -1.3 3,228,976 + Psyr_2658 0.58 +0.3 3,228,976 + Psyr_2658 0.58 +0.1 3,228,977 - Psyr_2658 0.58 -0.9 3,228,977 - Psyr_2658 0.58 -0.6 3,228,977 - Psyr_2658 0.58 +0.6 3,228,998 + Psyr_2658 0.60 +0.3 3,228,999 - Psyr_2658 0.60 -1.3 3,229,027 + Psyr_2658 0.63 +1.6 3,229,028 - Psyr_2658 0.63 +0.2 3,229,028 - Psyr_2658 0.63 +2.2 3,229,028 - Psyr_2658 0.63 +2.5 3,229,089 + Psyr_2658 0.68 -1.7
Or see this region's nucleotide sequence