Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2623 and Psyr_2624 are separated by 55 nucleotides Psyr_2624 and Psyr_2625 are separated by 7 nucleotides Psyr_2625 and Psyr_2626 are separated by 32 nucleotides
Psyr_2623: Psyr_2623 - conserved hypothetical protein, at 3,182,993 to 3,184,252
_2623
Psyr_2624: Psyr_2624 - lipoprotein, putative, at 3,184,308 to 3,184,808
_2624
Psyr_2625: Psyr_2625 - lipoprotein, putative, at 3,184,816 to 3,185,553
_2625
Psyr_2626: Psyr_2626 - Protein of unknown function DUF323, at 3,185,586 to 3,187,133
_2626
Position (kb)
3184
3185 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3183.565 kb on - strand, within Psyr_2623 at 3183.565 kb on - strand, within Psyr_2623 at 3183.592 kb on + strand, within Psyr_2623 at 3183.673 kb on - strand, within Psyr_2623 at 3183.673 kb on - strand, within Psyr_2623 at 3183.673 kb on - strand, within Psyr_2623 at 3183.677 kb on - strand, within Psyr_2623 at 3183.740 kb on + strand, within Psyr_2623 at 3183.741 kb on - strand, within Psyr_2623 at 3183.741 kb on - strand, within Psyr_2623 at 3183.743 kb on + strand, within Psyr_2623 at 3183.744 kb on - strand, within Psyr_2623 at 3183.764 kb on + strand, within Psyr_2623 at 3183.904 kb on + strand, within Psyr_2623 at 3183.904 kb on + strand, within Psyr_2623 at 3183.904 kb on + strand, within Psyr_2623 at 3183.905 kb on - strand, within Psyr_2623 at 3183.906 kb on + strand, within Psyr_2623 at 3183.907 kb on - strand, within Psyr_2623 at 3183.949 kb on + strand, within Psyr_2623 at 3183.968 kb on - strand, within Psyr_2623 at 3184.298 kb on + strand at 3184.302 kb on + strand at 3184.303 kb on - strand at 3184.327 kb on - strand at 3184.327 kb on - strand at 3184.327 kb on - strand at 3184.327 kb on - strand at 3184.597 kb on + strand, within Psyr_2624 at 3184.799 kb on + strand at 3184.802 kb on + strand at 3184.803 kb on - strand at 3184.848 kb on - strand at 3184.938 kb on + strand, within Psyr_2625 at 3184.970 kb on + strand, within Psyr_2625 at 3184.970 kb on + strand, within Psyr_2625 at 3184.979 kb on - strand, within Psyr_2625 at 3185.007 kb on + strand, within Psyr_2625 at 3185.144 kb on + strand, within Psyr_2625 at 3185.144 kb on + strand, within Psyr_2625 at 3185.145 kb on - strand, within Psyr_2625 at 3185.145 kb on - strand, within Psyr_2625 at 3185.145 kb on - strand, within Psyr_2625 at 3185.148 kb on + strand, within Psyr_2625 at 3185.149 kb on - strand, within Psyr_2625 at 3185.179 kb on + strand, within Psyr_2625 at 3185.222 kb on + strand, within Psyr_2625 at 3185.222 kb on + strand, within Psyr_2625 at 3185.223 kb on - strand, within Psyr_2625 at 3185.243 kb on + strand, within Psyr_2625 at 3185.243 kb on + strand, within Psyr_2625 at 3185.243 kb on + strand, within Psyr_2625 at 3185.244 kb on - strand, within Psyr_2625 at 3185.246 kb on + strand, within Psyr_2625 at 3185.246 kb on + strand, within Psyr_2625 at 3185.249 kb on + strand, within Psyr_2625 at 3185.249 kb on + strand, within Psyr_2625 at 3185.249 kb on + strand, within Psyr_2625 at 3185.249 kb on + strand, within Psyr_2625 at 3185.249 kb on + strand, within Psyr_2625 at 3185.250 kb on - strand, within Psyr_2625 at 3185.250 kb on - strand, within Psyr_2625 at 3185.343 kb on + strand, within Psyr_2625 at 3185.347 kb on + strand, within Psyr_2625 at 3185.542 kb on + strand at 3185.543 kb on - strand at 3185.543 kb on - strand at 3185.543 kb on - strand at 3185.543 kb on - strand at 3185.543 kb on - strand at 3185.543 kb on - strand at 3185.587 kb on + strand at 3185.601 kb on - strand at 3185.628 kb on + strand at 3185.629 kb on - strand at 3185.781 kb on + strand, within Psyr_2626 at 3185.797 kb on + strand, within Psyr_2626
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 3,183,565 - Psyr_2623 0.45 +1.1 3,183,565 - Psyr_2623 0.45 -0.1 3,183,592 + Psyr_2623 0.48 -1.1 3,183,673 - Psyr_2623 0.54 +0.5 3,183,673 - Psyr_2623 0.54 -1.1 3,183,673 - Psyr_2623 0.54 -1.0 3,183,677 - Psyr_2623 0.54 +0.5 3,183,740 + Psyr_2623 0.59 +0.5 3,183,741 - Psyr_2623 0.59 -1.1 3,183,741 - Psyr_2623 0.59 -0.4 3,183,743 + Psyr_2623 0.60 -0.8 3,183,744 - Psyr_2623 0.60 +1.5 3,183,764 + Psyr_2623 0.61 +0.7 3,183,904 + Psyr_2623 0.72 -1.0 3,183,904 + Psyr_2623 0.72 +2.3 3,183,904 + Psyr_2623 0.72 +1.1 3,183,905 - Psyr_2623 0.72 -0.0 3,183,906 + Psyr_2623 0.72 +1.5 3,183,907 - Psyr_2623 0.73 +2.1 3,183,949 + Psyr_2623 0.76 +1.7 3,183,968 - Psyr_2623 0.77 -1.1 3,184,298 + -1.7 3,184,302 + -1.5 3,184,303 - -0.4 3,184,327 - -0.2 3,184,327 - +1.0 3,184,327 - -1.1 3,184,327 - -0.1 3,184,597 + Psyr_2624 0.58 -0.5 3,184,799 + -0.5 3,184,802 + -0.8 3,184,803 - +0.5 3,184,848 - -0.1 3,184,938 + Psyr_2625 0.17 -0.0 3,184,970 + Psyr_2625 0.21 -0.6 3,184,970 + Psyr_2625 0.21 -0.9 3,184,979 - Psyr_2625 0.22 -1.2 3,185,007 + Psyr_2625 0.26 -0.3 3,185,144 + Psyr_2625 0.44 +0.5 3,185,144 + Psyr_2625 0.44 -0.6 3,185,145 - Psyr_2625 0.45 -1.2 3,185,145 - Psyr_2625 0.45 -0.2 3,185,145 - Psyr_2625 0.45 -1.7 3,185,148 + Psyr_2625 0.45 +0.5 3,185,149 - Psyr_2625 0.45 -0.7 3,185,179 + Psyr_2625 0.49 -0.5 3,185,222 + Psyr_2625 0.55 +0.4 3,185,222 + Psyr_2625 0.55 +1.5 3,185,223 - Psyr_2625 0.55 +1.5 3,185,243 + Psyr_2625 0.58 +1.2 3,185,243 + Psyr_2625 0.58 +0.9 3,185,243 + Psyr_2625 0.58 -0.3 3,185,244 - Psyr_2625 0.58 -0.2 3,185,246 + Psyr_2625 0.58 -0.9 3,185,246 + Psyr_2625 0.58 -1.3 3,185,249 + Psyr_2625 0.59 +2.5 3,185,249 + Psyr_2625 0.59 -0.3 3,185,249 + Psyr_2625 0.59 +0.6 3,185,249 + Psyr_2625 0.59 +1.5 3,185,249 + Psyr_2625 0.59 +3.1 3,185,250 - Psyr_2625 0.59 -0.7 3,185,250 - Psyr_2625 0.59 -1.3 3,185,343 + Psyr_2625 0.71 +0.6 3,185,347 + Psyr_2625 0.72 -0.0 3,185,542 + -0.9 3,185,543 - -0.4 3,185,543 - +0.5 3,185,543 - -0.1 3,185,543 - -1.4 3,185,543 - -0.7 3,185,543 - -2.1 3,185,587 + -2.1 3,185,601 - -1.1 3,185,628 + -0.1 3,185,629 - +0.2 3,185,781 + Psyr_2626 0.13 -0.5 3,185,797 + Psyr_2626 0.14 -0.5
Or see this region's nucleotide sequence