Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_2500

Experiment: KB with Aristolochic Acid 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_2498 and Psyr_2499 are separated by 30 nucleotidesPsyr_2499 and Psyr_2500 are separated by 70 nucleotidesPsyr_2500 and Psyr_2501 are separated by 76 nucleotidesPsyr_2501 and Psyr_2502 are separated by 48 nucleotides Psyr_2498: Psyr_2498 - GCN5-related N-acetyltransferase, at 2,909,986 to 2,910,531 _2498 Psyr_2499: Psyr_2499 - Glutathione S-transferase, N-terminal:Glutathione S-transferase, C-terminal, at 2,910,562 to 2,911,185 _2499 Psyr_2500: Psyr_2500 - conserved hypothetical protein, at 2,911,256 to 2,911,681 _2500 Psyr_2501: Psyr_2501 - Hemerythrin, at 2,911,758 to 2,912,240 _2501 Psyr_2502: Psyr_2502 - conserved hypothetical protein, at 2,912,289 to 2,912,648 _2502 Position (kb) 2911 2912Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 2910.292 kb on + strand, within Psyr_2498at 2910.293 kb on - strand, within Psyr_2498at 2910.335 kb on + strand, within Psyr_2498at 2910.427 kb on + strand, within Psyr_2498at 2910.428 kb on - strand, within Psyr_2498at 2910.442 kb on + strand, within Psyr_2498at 2910.442 kb on + strand, within Psyr_2498at 2910.456 kb on + strand, within Psyr_2498at 2910.466 kb on + strand, within Psyr_2498at 2910.466 kb on + strand, within Psyr_2498at 2910.466 kb on + strand, within Psyr_2498at 2910.466 kb on + strand, within Psyr_2498at 2910.467 kb on - strand, within Psyr_2498at 2910.467 kb on - strandat 2910.573 kb on - strandat 2910.573 kb on - strandat 2910.599 kb on + strandat 2910.599 kb on + strandat 2910.635 kb on + strand, within Psyr_2499at 2910.635 kb on + strand, within Psyr_2499at 2910.636 kb on - strand, within Psyr_2499at 2910.636 kb on - strand, within Psyr_2499at 2910.775 kb on + strand, within Psyr_2499at 2910.890 kb on - strand, within Psyr_2499at 2910.890 kb on - strand, within Psyr_2499at 2910.909 kb on - strand, within Psyr_2499at 2910.975 kb on + strand, within Psyr_2499at 2910.976 kb on - strand, within Psyr_2499at 2910.983 kb on + strand, within Psyr_2499at 2910.983 kb on + strand, within Psyr_2499at 2910.984 kb on - strand, within Psyr_2499at 2911.015 kb on + strand, within Psyr_2499at 2911.016 kb on - strand, within Psyr_2499at 2911.016 kb on - strand, within Psyr_2499at 2911.016 kb on - strand, within Psyr_2499at 2911.123 kb on + strandat 2911.127 kb on - strandat 2911.525 kb on + strand, within Psyr_2500at 2911.715 kb on + strandat 2911.716 kb on - strandat 2911.749 kb on + strandat 2911.750 kb on - strandat 2911.956 kb on + strand, within Psyr_2501at 2911.956 kb on + strand, within Psyr_2501at 2911.956 kb on + strand, within Psyr_2501at 2911.956 kb on + strand, within Psyr_2501at 2912.258 kb on - strandat 2912.385 kb on + strand, within Psyr_2502at 2912.538 kb on + strand, within Psyr_2502at 2912.538 kb on + strand, within Psyr_2502at 2912.539 kb on - strand, within Psyr_2502at 2912.539 kb on - strand, within Psyr_2502at 2912.586 kb on + strand, within Psyr_2502at 2912.587 kb on - strand, within Psyr_2502at 2912.587 kb on - strand, within Psyr_2502at 2912.587 kb on - strand, within Psyr_2502at 2912.637 kb on - strandat 2912.637 kb on - strandat 2912.637 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM
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2,910,292 + Psyr_2498 0.56 +0.7
2,910,293 - Psyr_2498 0.56 -1.7
2,910,335 + Psyr_2498 0.64 +0.5
2,910,427 + Psyr_2498 0.81 +0.7
2,910,428 - Psyr_2498 0.81 +0.9
2,910,442 + Psyr_2498 0.84 +1.4
2,910,442 + Psyr_2498 0.84 +0.5
2,910,456 + Psyr_2498 0.86 -1.5
2,910,466 + Psyr_2498 0.88 +0.3
2,910,466 + Psyr_2498 0.88 -0.3
2,910,466 + Psyr_2498 0.88 -0.5
2,910,466 + Psyr_2498 0.88 -0.0
2,910,467 - Psyr_2498 0.88 -1.8
2,910,467 - +4.7
2,910,573 - -1.4
2,910,573 - +1.5
2,910,599 + +0.4
2,910,599 + +0.8
2,910,635 + Psyr_2499 0.12 +1.4
2,910,635 + Psyr_2499 0.12 -0.2
2,910,636 - Psyr_2499 0.12 -0.1
2,910,636 - Psyr_2499 0.12 -2.5
2,910,775 + Psyr_2499 0.34 +0.4
2,910,890 - Psyr_2499 0.53 -0.9
2,910,890 - Psyr_2499 0.53 -0.2
2,910,909 - Psyr_2499 0.56 +0.9
2,910,975 + Psyr_2499 0.66 -1.4
2,910,976 - Psyr_2499 0.66 -0.1
2,910,983 + Psyr_2499 0.67 -0.1
2,910,983 + Psyr_2499 0.67 +0.7
2,910,984 - Psyr_2499 0.68 -0.6
2,911,015 + Psyr_2499 0.73 -0.0
2,911,016 - Psyr_2499 0.73 +1.0
2,911,016 - Psyr_2499 0.73 +1.0
2,911,016 - Psyr_2499 0.73 +0.5
2,911,123 + -0.3
2,911,127 - +0.1
2,911,525 + Psyr_2500 0.63 -0.7
2,911,715 + -1.6
2,911,716 - +1.4
2,911,749 + +1.4
2,911,750 - -1.8
2,911,956 + Psyr_2501 0.41 +0.4
2,911,956 + Psyr_2501 0.41 +0.4
2,911,956 + Psyr_2501 0.41 -0.7
2,911,956 + Psyr_2501 0.41 -0.1
2,912,258 - -1.2
2,912,385 + Psyr_2502 0.27 +0.2
2,912,538 + Psyr_2502 0.69 +0.8
2,912,538 + Psyr_2502 0.69 -0.2
2,912,539 - Psyr_2502 0.69 -0.6
2,912,539 - Psyr_2502 0.69 -0.9
2,912,586 + Psyr_2502 0.82 +0.4
2,912,587 - Psyr_2502 0.83 -0.8
2,912,587 - Psyr_2502 0.83 +1.4
2,912,587 - Psyr_2502 0.83 +1.0
2,912,637 - -0.4
2,912,637 - +0.3
2,912,637 - +0.4

Or see this region's nucleotide sequence