Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2474 and Psyr_2475 are separated by 11 nucleotides Psyr_2475 and Psyr_2476 are separated by 148 nucleotides
Psyr_2474: Psyr_2474 - Acyl-CoA dehydrogenase, C-terminal:Acyl-CoA dehydrogenase, central region, at 2,867,814 to 2,869,043
_2474
Psyr_2475: Psyr_2475 - phosphate ABC transporter substrate-binding protein, PhoT family, at 2,869,055 to 2,870,398
_2475
Psyr_2476: Psyr_2476 - Exodeoxyribonuclease III, at 2,870,547 to 2,871,359
_2476
Position (kb)
2869
2870
2871 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2868.771 kb on + strand, within Psyr_2474 at 2868.771 kb on + strand, within Psyr_2474 at 2868.772 kb on - strand, within Psyr_2474 at 2868.918 kb on + strand, within Psyr_2474 at 2868.918 kb on + strand, within Psyr_2474 at 2868.918 kb on + strand, within Psyr_2474 at 2868.918 kb on + strand, within Psyr_2474 at 2868.918 kb on + strand, within Psyr_2474 at 2868.918 kb on + strand, within Psyr_2474 at 2868.924 kb on + strand at 2868.924 kb on + strand at 2868.924 kb on + strand at 2868.938 kb on + strand at 2868.938 kb on + strand at 2868.938 kb on + strand at 2868.938 kb on + strand at 2868.939 kb on - strand at 2868.939 kb on - strand at 2868.993 kb on + strand at 2868.994 kb on - strand at 2868.994 kb on - strand at 2869.008 kb on + strand at 2869.008 kb on + strand at 2869.008 kb on + strand at 2869.008 kb on + strand at 2869.008 kb on + strand at 2869.008 kb on + strand at 2869.008 kb on + strand at 2869.008 kb on + strand at 2869.008 kb on + strand at 2869.008 kb on + strand at 2869.008 kb on + strand at 2869.008 kb on + strand at 2869.009 kb on - strand at 2869.009 kb on - strand at 2869.009 kb on - strand at 2869.009 kb on - strand at 2869.009 kb on - strand at 2869.009 kb on - strand at 2869.009 kb on - strand at 2869.009 kb on - strand at 2869.009 kb on - strand at 2869.009 kb on - strand at 2869.009 kb on - strand at 2869.010 kb on + strand at 2869.010 kb on + strand at 2869.010 kb on + strand at 2869.011 kb on - strand at 2869.114 kb on + strand at 2869.257 kb on - strand, within Psyr_2475 at 2869.454 kb on + strand, within Psyr_2475 at 2869.455 kb on - strand, within Psyr_2475 at 2869.560 kb on - strand, within Psyr_2475 at 2869.560 kb on - strand, within Psyr_2475 at 2869.563 kb on + strand, within Psyr_2475 at 2869.563 kb on + strand, within Psyr_2475 at 2869.563 kb on + strand, within Psyr_2475 at 2869.564 kb on - strand, within Psyr_2475 at 2869.564 kb on - strand, within Psyr_2475 at 2869.564 kb on - strand, within Psyr_2475 at 2869.564 kb on - strand, within Psyr_2475 at 2869.564 kb on - strand, within Psyr_2475 at 2869.569 kb on + strand, within Psyr_2475 at 2869.569 kb on + strand, within Psyr_2475 at 2869.570 kb on - strand, within Psyr_2475 at 2869.815 kb on + strand, within Psyr_2475 at 2869.816 kb on - strand, within Psyr_2475 at 2869.842 kb on + strand, within Psyr_2475 at 2869.843 kb on - strand, within Psyr_2475 at 2869.843 kb on - strand, within Psyr_2475 at 2870.307 kb on - strand at 2870.436 kb on + strand at 2870.436 kb on + strand at 2870.437 kb on - strand at 2870.437 kb on - strand at 2870.437 kb on - strand at 2870.458 kb on + strand at 2870.478 kb on + strand at 2870.478 kb on + strand at 2870.478 kb on + strand at 2870.478 kb on + strand at 2870.479 kb on - strand at 2870.479 kb on - strand at 2870.504 kb on + strand at 2870.507 kb on + strand at 2871.074 kb on - strand, within Psyr_2476 at 2871.074 kb on - strand, within Psyr_2476 at 2871.074 kb on - strand, within Psyr_2476 at 2871.074 kb on - strand, within Psyr_2476 at 2871.149 kb on + strand, within Psyr_2476 at 2871.149 kb on + strand, within Psyr_2476 at 2871.150 kb on - strand, within Psyr_2476
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 2,868,771 + Psyr_2474 0.78 +0.4 2,868,771 + Psyr_2474 0.78 +1.4 2,868,772 - Psyr_2474 0.78 +0.4 2,868,918 + Psyr_2474 0.90 +0.1 2,868,918 + Psyr_2474 0.90 -0.4 2,868,918 + Psyr_2474 0.90 -0.4 2,868,918 + Psyr_2474 0.90 +2.0 2,868,918 + Psyr_2474 0.90 +1.4 2,868,918 + Psyr_2474 0.90 +2.2 2,868,924 + -0.6 2,868,924 + +1.4 2,868,924 + -0.5 2,868,938 + -0.2 2,868,938 + -0.3 2,868,938 + +0.5 2,868,938 + +2.0 2,868,939 - +0.4 2,868,939 - -1.8 2,868,993 + -1.2 2,868,994 - -1.6 2,868,994 - -1.1 2,869,008 + +1.1 2,869,008 + -2.2 2,869,008 + +0.5 2,869,008 + -0.7 2,869,008 + -1.8 2,869,008 + +0.4 2,869,008 + +1.4 2,869,008 + +0.1 2,869,008 + +1.2 2,869,008 + -0.4 2,869,008 + -0.4 2,869,008 + -2.2 2,869,009 - +2.4 2,869,009 - -0.3 2,869,009 - -1.8 2,869,009 - +0.4 2,869,009 - -0.0 2,869,009 - -0.0 2,869,009 - -2.1 2,869,009 - -2.7 2,869,009 - +3.4 2,869,009 - -1.4 2,869,009 - -0.5 2,869,010 + -0.5 2,869,010 + -0.2 2,869,010 + +0.7 2,869,011 - +1.4 2,869,114 + +0.4 2,869,257 - Psyr_2475 0.15 +0.7 2,869,454 + Psyr_2475 0.30 -0.0 2,869,455 - Psyr_2475 0.30 -1.3 2,869,560 - Psyr_2475 0.38 -0.1 2,869,560 - Psyr_2475 0.38 -0.1 2,869,563 + Psyr_2475 0.38 +0.5 2,869,563 + Psyr_2475 0.38 +0.7 2,869,563 + Psyr_2475 0.38 +0.8 2,869,564 - Psyr_2475 0.38 +1.4 2,869,564 - Psyr_2475 0.38 -0.5 2,869,564 - Psyr_2475 0.38 +0.1 2,869,564 - Psyr_2475 0.38 -0.9 2,869,564 - Psyr_2475 0.38 -0.6 2,869,569 + Psyr_2475 0.38 +1.2 2,869,569 + Psyr_2475 0.38 +1.1 2,869,570 - Psyr_2475 0.38 -0.9 2,869,815 + Psyr_2475 0.57 +0.2 2,869,816 - Psyr_2475 0.57 -0.3 2,869,842 + Psyr_2475 0.59 -1.4 2,869,843 - Psyr_2475 0.59 +1.2 2,869,843 - Psyr_2475 0.59 +1.2 2,870,307 - +0.4 2,870,436 + -0.4 2,870,436 + -0.3 2,870,437 - -0.9 2,870,437 - -1.4 2,870,437 - -0.9 2,870,458 + +0.0 2,870,478 + +0.1 2,870,478 + -0.0 2,870,478 + +0.2 2,870,478 + +0.8 2,870,479 - -0.9 2,870,479 - -0.8 2,870,504 + +1.4 2,870,507 + -0.6 2,871,074 - Psyr_2476 0.65 -0.2 2,871,074 - Psyr_2476 0.65 -1.2 2,871,074 - Psyr_2476 0.65 -2.4 2,871,074 - Psyr_2476 0.65 +0.4 2,871,149 + Psyr_2476 0.74 +2.0 2,871,149 + Psyr_2476 0.74 +1.0 2,871,150 - Psyr_2476 0.74 +1.0
Or see this region's nucleotide sequence