Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2471 and Psyr_2472 are separated by 30 nucleotides Psyr_2472 and Psyr_2473 are separated by 165 nucleotides Psyr_2473 and Psyr_2474 are separated by 185 nucleotides
Psyr_2471: Psyr_2471 - hydroxymethylglutaryl-CoA lyase, at 2,865,211 to 2,866,110
_2471
Psyr_2472: Psyr_2472 - transcriptional regulator, MerR family, at 2,866,141 to 2,866,533
_2472
Psyr_2473: Psyr_2473 - transcriptional regulator, LysR family, at 2,866,699 to 2,867,628
_2473
Psyr_2474: Psyr_2474 - Acyl-CoA dehydrogenase, C-terminal:Acyl-CoA dehydrogenase, central region, at 2,867,814 to 2,869,043
_2474
Position (kb)
2866
2867
2868 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2866.125 kb on - strand at 2866.125 kb on - strand at 2866.316 kb on + strand, within Psyr_2472 at 2866.317 kb on - strand, within Psyr_2472 at 2866.409 kb on + strand, within Psyr_2472 at 2866.410 kb on - strand, within Psyr_2472 at 2866.410 kb on - strand, within Psyr_2472 at 2866.410 kb on - strand, within Psyr_2472 at 2866.410 kb on - strand, within Psyr_2472 at 2866.410 kb on - strand, within Psyr_2472 at 2866.410 kb on - strand, within Psyr_2472 at 2866.413 kb on + strand, within Psyr_2472 at 2866.414 kb on - strand, within Psyr_2472 at 2866.414 kb on - strand, within Psyr_2472 at 2866.414 kb on - strand, within Psyr_2472 at 2866.414 kb on - strand, within Psyr_2472 at 2866.414 kb on - strand, within Psyr_2472 at 2866.414 kb on - strand, within Psyr_2472 at 2866.414 kb on - strand, within Psyr_2472 at 2866.414 kb on - strand, within Psyr_2472 at 2866.414 kb on - strand, within Psyr_2472 at 2866.414 kb on - strand, within Psyr_2472 at 2866.518 kb on - strand at 2866.518 kb on - strand at 2866.552 kb on - strand at 2866.552 kb on - strand at 2866.553 kb on + strand at 2866.554 kb on - strand at 2866.554 kb on - strand at 2866.554 kb on - strand at 2866.554 kb on - strand at 2866.554 kb on - strand at 2866.554 kb on - strand at 2866.554 kb on - strand at 2866.850 kb on + strand, within Psyr_2473 at 2866.850 kb on + strand, within Psyr_2473 at 2866.856 kb on + strand, within Psyr_2473 at 2866.856 kb on + strand, within Psyr_2473 at 2866.856 kb on + strand, within Psyr_2473 at 2866.856 kb on + strand, within Psyr_2473 at 2866.856 kb on + strand, within Psyr_2473 at 2866.857 kb on - strand, within Psyr_2473 at 2866.857 kb on - strand, within Psyr_2473 at 2866.857 kb on - strand, within Psyr_2473 at 2866.857 kb on - strand, within Psyr_2473 at 2866.857 kb on - strand, within Psyr_2473 at 2866.857 kb on - strand, within Psyr_2473 at 2866.940 kb on + strand, within Psyr_2473 at 2866.940 kb on + strand, within Psyr_2473 at 2866.940 kb on + strand, within Psyr_2473 at 2866.940 kb on + strand, within Psyr_2473 at 2866.940 kb on + strand, within Psyr_2473 at 2866.940 kb on + strand, within Psyr_2473 at 2866.940 kb on + strand, within Psyr_2473 at 2866.940 kb on + strand, within Psyr_2473 at 2866.940 kb on + strand, within Psyr_2473 at 2866.941 kb on - strand, within Psyr_2473 at 2866.941 kb on - strand, within Psyr_2473 at 2866.941 kb on - strand, within Psyr_2473 at 2866.941 kb on - strand, within Psyr_2473 at 2866.941 kb on - strand, within Psyr_2473 at 2867.031 kb on + strand, within Psyr_2473 at 2867.031 kb on + strand, within Psyr_2473 at 2867.037 kb on + strand, within Psyr_2473 at 2867.038 kb on - strand, within Psyr_2473 at 2867.118 kb on - strand, within Psyr_2473 at 2867.576 kb on + strand at 2867.577 kb on - strand at 2867.577 kb on - strand at 2867.667 kb on + strand at 2867.702 kb on + strand at 2867.702 kb on + strand at 2867.702 kb on + strand at 2867.702 kb on + strand at 2867.703 kb on - strand at 2867.724 kb on - strand at 2867.741 kb on + strand at 2867.741 kb on + strand at 2867.742 kb on - strand at 2867.742 kb on - strand at 2867.764 kb on + strand at 2867.765 kb on - strand at 2867.880 kb on + strand at 2867.880 kb on + strand at 2867.880 kb on + strand at 2867.881 kb on - strand at 2867.881 kb on - strand at 2867.881 kb on - strand at 2868.045 kb on + strand, within Psyr_2474 at 2868.046 kb on - strand, within Psyr_2474
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 2,866,125 - -0.8 2,866,125 - +1.1 2,866,316 + Psyr_2472 0.45 +1.1 2,866,317 - Psyr_2472 0.45 -0.1 2,866,409 + Psyr_2472 0.68 +0.4 2,866,410 - Psyr_2472 0.68 -0.5 2,866,410 - Psyr_2472 0.68 -0.4 2,866,410 - Psyr_2472 0.68 +0.4 2,866,410 - Psyr_2472 0.68 -0.3 2,866,410 - Psyr_2472 0.68 +2.4 2,866,410 - Psyr_2472 0.68 -1.8 2,866,413 + Psyr_2472 0.69 -1.8 2,866,414 - Psyr_2472 0.69 -0.8 2,866,414 - Psyr_2472 0.69 -0.1 2,866,414 - Psyr_2472 0.69 +1.4 2,866,414 - Psyr_2472 0.69 -0.5 2,866,414 - Psyr_2472 0.69 -2.3 2,866,414 - Psyr_2472 0.69 +1.7 2,866,414 - Psyr_2472 0.69 -1.1 2,866,414 - Psyr_2472 0.69 -1.6 2,866,414 - Psyr_2472 0.69 +0.4 2,866,414 - Psyr_2472 0.69 -0.9 2,866,518 - -0.9 2,866,518 - +1.4 2,866,552 - +1.4 2,866,552 - -0.9 2,866,553 + -0.6 2,866,554 - -1.2 2,866,554 - -0.9 2,866,554 - -1.2 2,866,554 - -1.2 2,866,554 - -0.8 2,866,554 - -1.9 2,866,554 - -1.1 2,866,850 + Psyr_2473 0.16 -0.3 2,866,850 + Psyr_2473 0.16 +2.0 2,866,856 + Psyr_2473 0.17 -1.4 2,866,856 + Psyr_2473 0.17 +0.9 2,866,856 + Psyr_2473 0.17 -1.7 2,866,856 + Psyr_2473 0.17 -1.2 2,866,856 + Psyr_2473 0.17 -0.2 2,866,857 - Psyr_2473 0.17 -0.1 2,866,857 - Psyr_2473 0.17 +0.0 2,866,857 - Psyr_2473 0.17 -0.2 2,866,857 - Psyr_2473 0.17 +1.9 2,866,857 - Psyr_2473 0.17 -0.3 2,866,857 - Psyr_2473 0.17 -0.2 2,866,940 + Psyr_2473 0.26 -0.6 2,866,940 + Psyr_2473 0.26 -0.6 2,866,940 + Psyr_2473 0.26 -1.0 2,866,940 + Psyr_2473 0.26 -1.4 2,866,940 + Psyr_2473 0.26 -0.0 2,866,940 + Psyr_2473 0.26 -1.1 2,866,940 + Psyr_2473 0.26 +0.2 2,866,940 + Psyr_2473 0.26 +1.0 2,866,940 + Psyr_2473 0.26 +1.0 2,866,941 - Psyr_2473 0.26 -1.2 2,866,941 - Psyr_2473 0.26 -0.9 2,866,941 - Psyr_2473 0.26 -0.6 2,866,941 - Psyr_2473 0.26 -0.3 2,866,941 - Psyr_2473 0.26 -0.0 2,867,031 + Psyr_2473 0.36 -1.0 2,867,031 + Psyr_2473 0.36 +0.3 2,867,037 + Psyr_2473 0.36 -1.2 2,867,038 - Psyr_2473 0.36 -0.0 2,867,118 - Psyr_2473 0.45 -0.5 2,867,576 + +0.1 2,867,577 - -0.9 2,867,577 - +0.7 2,867,667 + +1.2 2,867,702 + +0.7 2,867,702 + -0.4 2,867,702 + +3.0 2,867,702 + -0.2 2,867,703 - +0.4 2,867,724 - -0.9 2,867,741 + +2.7 2,867,741 + +0.5 2,867,742 - -2.2 2,867,742 - -0.2 2,867,764 + -2.6 2,867,765 - +0.1 2,867,880 + +0.1 2,867,880 + -0.3 2,867,880 + -0.1 2,867,881 - -0.9 2,867,881 - +1.4 2,867,881 - -1.3 2,868,045 + Psyr_2474 0.19 -0.6 2,868,046 - Psyr_2474 0.19 -0.6
Or see this region's nucleotide sequence