Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2348 and Psyr_2349 are separated by 451 nucleotides Psyr_2349 and Psyr_2350 are separated by 411 nucleotides Psyr_2350 and Psyr_2351 are separated by 78 nucleotides
Psyr_2348: Psyr_2348 - hypothetical protein, at 2,724,625 to 2,725,374
_2348
Psyr_2349: Psyr_2349 - hypothetical protein, at 2,725,826 to 2,726,128
_2349
Psyr_2350: Psyr_2350 - Protein of unknown function UPF0025, at 2,726,540 to 2,726,998
_2350
Psyr_2351: Psyr_2351 - tRNA synthetase, class-II (G, H, P and S), at 2,727,077 to 2,727,658
_2351
Position (kb)
2725
2726
2727 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2724.849 kb on - strand, within Psyr_2348 at 2724.878 kb on - strand, within Psyr_2348 at 2725.195 kb on - strand, within Psyr_2348 at 2725.206 kb on + strand, within Psyr_2348 at 2725.207 kb on - strand, within Psyr_2348 at 2725.207 kb on - strand, within Psyr_2348 at 2725.220 kb on + strand, within Psyr_2348 at 2725.287 kb on + strand, within Psyr_2348 at 2725.288 kb on - strand, within Psyr_2348 at 2725.335 kb on + strand at 2725.339 kb on - strand at 2725.340 kb on + strand at 2725.340 kb on + strand at 2725.340 kb on + strand at 2725.341 kb on - strand at 2725.341 kb on - strand at 2725.345 kb on + strand at 2725.381 kb on - strand at 2725.387 kb on - strand at 2725.432 kb on + strand at 2725.467 kb on - strand at 2725.508 kb on + strand at 2725.509 kb on - strand at 2725.514 kb on + strand at 2725.533 kb on + strand at 2725.534 kb on - strand at 2725.599 kb on - strand at 2725.599 kb on - strand at 2725.605 kb on - strand at 2725.795 kb on - strand at 2725.805 kb on + strand at 2725.805 kb on + strand at 2725.805 kb on + strand at 2725.805 kb on + strand at 2725.805 kb on + strand at 2725.805 kb on + strand at 2725.805 kb on + strand at 2725.806 kb on - strand at 2725.806 kb on - strand at 2725.806 kb on - strand at 2725.842 kb on + strand at 2725.842 kb on + strand at 2725.842 kb on + strand at 2725.843 kb on - strand at 2725.910 kb on + strand, within Psyr_2349 at 2726.126 kb on + strand at 2726.126 kb on + strand at 2726.168 kb on - strand at 2726.168 kb on - strand at 2726.173 kb on + strand at 2726.173 kb on + strand at 2726.174 kb on - strand at 2726.180 kb on + strand at 2726.180 kb on + strand at 2726.180 kb on + strand at 2726.180 kb on + strand at 2726.181 kb on - strand at 2726.181 kb on - strand at 2726.181 kb on - strand at 2726.181 kb on - strand at 2726.181 kb on - strand at 2726.181 kb on - strand at 2726.210 kb on + strand at 2726.211 kb on - strand at 2726.236 kb on - strand at 2726.242 kb on + strand at 2726.268 kb on - strand at 2726.288 kb on + strand at 2726.426 kb on + strand at 2726.426 kb on + strand at 2726.426 kb on + strand at 2726.431 kb on - strand at 2726.451 kb on + strand at 2726.451 kb on + strand at 2726.451 kb on + strand at 2726.451 kb on + strand at 2726.451 kb on + strand at 2726.451 kb on + strand at 2726.451 kb on + strand at 2726.452 kb on - strand at 2726.452 kb on - strand at 2726.452 kb on - strand at 2726.452 kb on - strand at 2726.452 kb on - strand at 2726.452 kb on - strand at 2726.452 kb on - strand at 2726.519 kb on + strand at 2726.594 kb on + strand, within Psyr_2350 at 2726.652 kb on + strand, within Psyr_2350 at 2726.652 kb on + strand, within Psyr_2350 at 2726.652 kb on + strand, within Psyr_2350 at 2726.653 kb on - strand, within Psyr_2350 at 2726.653 kb on - strand, within Psyr_2350 at 2727.026 kb on - strand at 2727.026 kb on - strand at 2727.039 kb on - strand at 2727.055 kb on - strand at 2727.064 kb on + strand at 2727.101 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 2,724,849 - Psyr_2348 0.30 -2.4 2,724,878 - Psyr_2348 0.34 -2.3 2,725,195 - Psyr_2348 0.76 +1.4 2,725,206 + Psyr_2348 0.77 -0.1 2,725,207 - Psyr_2348 0.78 -0.6 2,725,207 - Psyr_2348 0.78 -1.0 2,725,220 + Psyr_2348 0.79 -0.2 2,725,287 + Psyr_2348 0.88 -1.0 2,725,288 - Psyr_2348 0.88 +1.6 2,725,335 + +0.8 2,725,339 - +0.2 2,725,340 + -0.7 2,725,340 + -0.6 2,725,340 + +0.2 2,725,341 - +1.4 2,725,341 - -1.6 2,725,345 + -1.1 2,725,381 - -1.8 2,725,387 - +0.1 2,725,432 + -0.2 2,725,467 - -0.6 2,725,508 + +1.2 2,725,509 - -2.0 2,725,514 + +0.0 2,725,533 + +1.4 2,725,534 - +1.4 2,725,599 - +1.4 2,725,599 - +0.6 2,725,605 - -0.6 2,725,795 - +0.0 2,725,805 + -1.4 2,725,805 + -0.3 2,725,805 + -0.4 2,725,805 + -0.2 2,725,805 + +1.0 2,725,805 + +1.6 2,725,805 + +0.6 2,725,806 - -2.1 2,725,806 - -1.6 2,725,806 - +3.4 2,725,842 + -0.8 2,725,842 + +0.9 2,725,842 + -0.1 2,725,843 - +1.8 2,725,910 + Psyr_2349 0.28 -0.6 2,726,126 + -2.0 2,726,126 + +0.5 2,726,168 - -0.8 2,726,168 - +1.4 2,726,173 + -0.7 2,726,173 + +0.7 2,726,174 - -1.4 2,726,180 + -0.2 2,726,180 + -0.2 2,726,180 + +1.8 2,726,180 + +0.5 2,726,181 - +0.4 2,726,181 - +0.4 2,726,181 - +0.8 2,726,181 - +0.7 2,726,181 - -1.2 2,726,181 - +0.4 2,726,210 + -0.6 2,726,211 - +0.4 2,726,236 - -0.6 2,726,242 + +0.6 2,726,268 - +1.4 2,726,288 + +1.2 2,726,426 + +1.4 2,726,426 + -1.2 2,726,426 + -2.1 2,726,431 - -1.6 2,726,451 + -2.1 2,726,451 + +0.2 2,726,451 + +1.4 2,726,451 + -1.6 2,726,451 + +0.5 2,726,451 + -0.3 2,726,451 + -0.2 2,726,452 - +0.1 2,726,452 - +1.0 2,726,452 - -0.1 2,726,452 - +0.2 2,726,452 - +0.0 2,726,452 - +0.5 2,726,452 - -0.7 2,726,519 + +0.1 2,726,594 + Psyr_2350 0.12 -0.4 2,726,652 + Psyr_2350 0.24 +1.0 2,726,652 + Psyr_2350 0.24 -1.2 2,726,652 + Psyr_2350 0.24 +0.7 2,726,653 - Psyr_2350 0.25 -0.8 2,726,653 - Psyr_2350 0.25 +0.4 2,727,026 - -0.3 2,727,026 - -1.0 2,727,039 - -0.9 2,727,055 - +0.3 2,727,064 + +2.5 2,727,101 + +0.1
Or see this region's nucleotide sequence