Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2293 and Psyr_2294 are separated by 7 nucleotides Psyr_2294 and Psyr_2295 are separated by 88 nucleotides
Psyr_2293: Psyr_2293 - conserved hypothetical protein, at 2,664,682 to 2,665,752
_2293
Psyr_2294: Psyr_2294 - conserved hypothetical protein, at 2,665,760 to 2,666,185
_2294
Psyr_2295: Psyr_2295 - ABC transporter, transmembrane region:ABC transporter, at 2,666,274 to 2,668,085
_2295
Position (kb)
2665
2666
2667 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2665.049 kb on - strand, within Psyr_2293 at 2665.225 kb on + strand, within Psyr_2293 at 2665.226 kb on - strand, within Psyr_2293 at 2665.226 kb on - strand, within Psyr_2293 at 2665.279 kb on + strand, within Psyr_2293 at 2665.279 kb on + strand, within Psyr_2293 at 2665.279 kb on + strand, within Psyr_2293 at 2665.280 kb on - strand, within Psyr_2293 at 2665.280 kb on - strand, within Psyr_2293 at 2665.280 kb on - strand, within Psyr_2293 at 2665.280 kb on - strand, within Psyr_2293 at 2665.280 kb on - strand, within Psyr_2293 at 2665.298 kb on + strand, within Psyr_2293 at 2665.298 kb on + strand, within Psyr_2293 at 2665.298 kb on + strand, within Psyr_2293 at 2665.299 kb on - strand, within Psyr_2293 at 2665.318 kb on + strand, within Psyr_2293 at 2665.318 kb on + strand, within Psyr_2293 at 2665.318 kb on + strand, within Psyr_2293 at 2665.319 kb on - strand, within Psyr_2293 at 2665.319 kb on - strand, within Psyr_2293 at 2665.526 kb on + strand, within Psyr_2293 at 2665.526 kb on + strand, within Psyr_2293 at 2665.526 kb on + strand, within Psyr_2293 at 2665.527 kb on - strand, within Psyr_2293 at 2665.684 kb on - strand at 2665.928 kb on + strand, within Psyr_2294 at 2665.928 kb on + strand, within Psyr_2294 at 2665.929 kb on - strand, within Psyr_2294 at 2665.992 kb on + strand, within Psyr_2294 at 2665.993 kb on - strand, within Psyr_2294 at 2666.172 kb on + strand at 2666.172 kb on + strand at 2666.172 kb on + strand at 2666.172 kb on + strand at 2666.173 kb on - strand at 2666.173 kb on - strand at 2666.173 kb on - strand at 2666.173 kb on - strand at 2666.173 kb on - strand at 2666.177 kb on - strand at 2666.177 kb on - strand at 2666.177 kb on - strand at 2666.177 kb on - strand at 2666.221 kb on + strand at 2666.222 kb on - strand at 2666.273 kb on + strand at 2666.273 kb on + strand at 2666.274 kb on - strand at 2666.274 kb on - strand at 2666.274 kb on - strand at 2666.506 kb on - strand, within Psyr_2295 at 2666.506 kb on - strand, within Psyr_2295 at 2666.671 kb on - strand, within Psyr_2295 at 2666.756 kb on + strand, within Psyr_2295 at 2666.919 kb on + strand, within Psyr_2295 at 2666.920 kb on - strand, within Psyr_2295 at 2666.920 kb on - strand, within Psyr_2295 at 2666.949 kb on + strand, within Psyr_2295 at 2666.949 kb on + strand, within Psyr_2295 at 2666.949 kb on + strand, within Psyr_2295 at 2667.102 kb on + strand, within Psyr_2295 at 2667.102 kb on + strand, within Psyr_2295 at 2667.171 kb on + strand, within Psyr_2295 at 2667.171 kb on + strand, within Psyr_2295 at 2667.171 kb on + strand, within Psyr_2295 at 2667.172 kb on - strand, within Psyr_2295 at 2667.172 kb on - strand, within Psyr_2295 at 2667.172 kb on - strand, within Psyr_2295 at 2667.172 kb on - strand, within Psyr_2295
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 2,665,049 - Psyr_2293 0.34 -0.2 2,665,225 + Psyr_2293 0.51 +0.8 2,665,226 - Psyr_2293 0.51 +1.4 2,665,226 - Psyr_2293 0.51 -2.6 2,665,279 + Psyr_2293 0.56 -0.5 2,665,279 + Psyr_2293 0.56 +0.1 2,665,279 + Psyr_2293 0.56 -0.7 2,665,280 - Psyr_2293 0.56 +0.2 2,665,280 - Psyr_2293 0.56 -0.4 2,665,280 - Psyr_2293 0.56 -1.4 2,665,280 - Psyr_2293 0.56 -1.1 2,665,280 - Psyr_2293 0.56 -1.0 2,665,298 + Psyr_2293 0.58 -1.6 2,665,298 + Psyr_2293 0.58 -0.9 2,665,298 + Psyr_2293 0.58 +0.3 2,665,299 - Psyr_2293 0.58 +0.0 2,665,318 + Psyr_2293 0.59 -0.7 2,665,318 + Psyr_2293 0.59 +0.2 2,665,318 + Psyr_2293 0.59 -2.3 2,665,319 - Psyr_2293 0.59 -0.8 2,665,319 - Psyr_2293 0.59 +1.1 2,665,526 + Psyr_2293 0.79 -0.4 2,665,526 + Psyr_2293 0.79 +0.4 2,665,526 + Psyr_2293 0.79 -0.5 2,665,527 - Psyr_2293 0.79 +3.0 2,665,684 - +0.4 2,665,928 + Psyr_2294 0.39 -1.3 2,665,928 + Psyr_2294 0.39 -1.1 2,665,929 - Psyr_2294 0.40 -0.4 2,665,992 + Psyr_2294 0.54 -0.5 2,665,993 - Psyr_2294 0.55 -0.3 2,666,172 + -0.6 2,666,172 + +0.4 2,666,172 + -0.3 2,666,172 + -0.9 2,666,173 - +0.1 2,666,173 - +0.5 2,666,173 - +0.8 2,666,173 - -0.6 2,666,173 - +1.0 2,666,177 - -0.2 2,666,177 - +0.9 2,666,177 - -0.9 2,666,177 - -0.3 2,666,221 + +1.0 2,666,222 - -1.5 2,666,273 + +0.1 2,666,273 + +0.5 2,666,274 - -0.1 2,666,274 - -0.9 2,666,274 - +0.3 2,666,506 - Psyr_2295 0.13 +1.0 2,666,506 - Psyr_2295 0.13 +0.2 2,666,671 - Psyr_2295 0.22 +1.2 2,666,756 + Psyr_2295 0.27 -0.6 2,666,919 + Psyr_2295 0.36 -0.4 2,666,920 - Psyr_2295 0.36 +0.1 2,666,920 - Psyr_2295 0.36 -0.2 2,666,949 + Psyr_2295 0.37 +0.6 2,666,949 + Psyr_2295 0.37 +0.1 2,666,949 + Psyr_2295 0.37 +0.5 2,667,102 + Psyr_2295 0.46 -0.7 2,667,102 + Psyr_2295 0.46 -1.9 2,667,171 + Psyr_2295 0.50 +0.5 2,667,171 + Psyr_2295 0.50 -0.6 2,667,171 + Psyr_2295 0.50 -0.2 2,667,172 - Psyr_2295 0.50 -1.1 2,667,172 - Psyr_2295 0.50 -1.3 2,667,172 - Psyr_2295 0.50 -1.1 2,667,172 - Psyr_2295 0.50 -0.2
Or see this region's nucleotide sequence