Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2232 and Psyr_2233 are separated by 60 nucleotides Psyr_2233 and Psyr_2234 are separated by 2 nucleotides Psyr_2234 and Psyr_2235 are separated by 190 nucleotides
Psyr_2232: Psyr_2232 - Major facilitator superfamily, at 2,595,071 to 2,596,369
_2232
Psyr_2233: Psyr_2233 - Protein of unknown function DUF1289, at 2,596,430 to 2,596,612
_2233
Psyr_2234: Psyr_2234 - conserved hypothetical protein, at 2,596,615 to 2,596,986
_2234
Psyr_2235: Psyr_2235 - gluconolactonase, at 2,597,177 to 2,598,277
_2235
Position (kb)
2596
2597 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2595.822 kb on + strand, within Psyr_2232 at 2595.823 kb on - strand, within Psyr_2232 at 2595.835 kb on + strand, within Psyr_2232 at 2595.836 kb on - strand, within Psyr_2232 at 2596.224 kb on + strand, within Psyr_2232 at 2596.224 kb on + strand, within Psyr_2232 at 2596.224 kb on + strand, within Psyr_2232 at 2596.225 kb on - strand, within Psyr_2232 at 2596.225 kb on - strand, within Psyr_2232 at 2596.237 kb on - strand, within Psyr_2232 at 2596.340 kb on - strand at 2596.374 kb on - strand at 2596.377 kb on - strand at 2596.377 kb on - strand at 2596.394 kb on + strand at 2596.424 kb on + strand at 2597.025 kb on - strand at 2597.025 kb on - strand at 2597.062 kb on + strand at 2597.062 kb on + strand at 2597.062 kb on + strand at 2597.064 kb on + strand at 2597.064 kb on + strand at 2597.064 kb on + strand at 2597.065 kb on - strand at 2597.117 kb on + strand at 2597.124 kb on + strand at 2597.124 kb on + strand at 2597.124 kb on + strand at 2597.125 kb on - strand at 2597.125 kb on - strand at 2597.165 kb on + strand at 2597.166 kb on - strand at 2597.171 kb on + strand at 2597.172 kb on - strand at 2597.363 kb on - strand, within Psyr_2235 at 2597.370 kb on + strand, within Psyr_2235 at 2597.370 kb on + strand, within Psyr_2235 at 2597.370 kb on + strand, within Psyr_2235 at 2597.370 kb on + strand, within Psyr_2235 at 2597.371 kb on - strand, within Psyr_2235 at 2597.371 kb on - strand, within Psyr_2235 at 2597.377 kb on - strand, within Psyr_2235
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 2,595,822 + Psyr_2232 0.58 +0.2 2,595,823 - Psyr_2232 0.58 +0.1 2,595,835 + Psyr_2232 0.59 +1.4 2,595,836 - Psyr_2232 0.59 +2.5 2,596,224 + Psyr_2232 0.89 -1.1 2,596,224 + Psyr_2232 0.89 +0.3 2,596,224 + Psyr_2232 0.89 -0.6 2,596,225 - Psyr_2232 0.89 +0.6 2,596,225 - Psyr_2232 0.89 -0.5 2,596,237 - Psyr_2232 0.90 +0.8 2,596,340 - -2.2 2,596,374 - -1.4 2,596,377 - -0.8 2,596,377 - -1.9 2,596,394 + -2.2 2,596,424 + +0.9 2,597,025 - -1.4 2,597,025 - +0.4 2,597,062 + +0.3 2,597,062 + -0.0 2,597,062 + -0.5 2,597,064 + -0.4 2,597,064 + +0.9 2,597,064 + -0.6 2,597,065 - +1.4 2,597,117 + -0.4 2,597,124 + -1.2 2,597,124 + -0.1 2,597,124 + +0.4 2,597,125 - -0.9 2,597,125 - +1.4 2,597,165 + -0.1 2,597,166 - +2.4 2,597,171 + -1.2 2,597,172 - +0.4 2,597,363 - Psyr_2235 0.17 -1.7 2,597,370 + Psyr_2235 0.18 +0.3 2,597,370 + Psyr_2235 0.18 +0.5 2,597,370 + Psyr_2235 0.18 +0.8 2,597,370 + Psyr_2235 0.18 +0.3 2,597,371 - Psyr_2235 0.18 -0.5 2,597,371 - Psyr_2235 0.18 -0.2 2,597,377 - Psyr_2235 0.18 -0.5
Or see this region's nucleotide sequence