Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2208 and Psyr_2209 are separated by 60 nucleotides Psyr_2209 and Psyr_2210 are separated by 76 nucleotides
Psyr_2208: Psyr_2208 - Amidase, at 2,565,581 to 2,567,116
_2208
Psyr_2209: Psyr_2209 - ABC transporter, substrate-binding protein, aliphatic sulfonate, at 2,567,177 to 2,568,151
_2209
Psyr_2210: Psyr_2210 - D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding protein, at 2,568,228 to 2,569,187
_2210
Position (kb)
2567
2568
2569 Strain fitness (log2 ratio)
-2
-1
0
1 at 2566.210 kb on + strand, within Psyr_2208 at 2566.214 kb on + strand, within Psyr_2208 at 2566.214 kb on + strand, within Psyr_2208 at 2566.229 kb on + strand, within Psyr_2208 at 2566.230 kb on - strand, within Psyr_2208 at 2566.643 kb on + strand, within Psyr_2208 at 2566.643 kb on + strand, within Psyr_2208 at 2566.644 kb on - strand, within Psyr_2208 at 2566.664 kb on + strand, within Psyr_2208 at 2566.665 kb on - strand, within Psyr_2208 at 2566.739 kb on + strand, within Psyr_2208 at 2566.767 kb on - strand, within Psyr_2208 at 2566.811 kb on + strand, within Psyr_2208 at 2566.846 kb on - strand, within Psyr_2208 at 2566.907 kb on + strand, within Psyr_2208 at 2566.907 kb on + strand, within Psyr_2208 at 2566.908 kb on - strand, within Psyr_2208 at 2566.908 kb on - strand, within Psyr_2208 at 2566.908 kb on - strand, within Psyr_2208 at 2567.073 kb on - strand at 2567.302 kb on - strand, within Psyr_2209 at 2567.481 kb on + strand, within Psyr_2209 at 2567.482 kb on - strand, within Psyr_2209 at 2567.494 kb on - strand, within Psyr_2209 at 2567.691 kb on + strand, within Psyr_2209 at 2567.745 kb on - strand, within Psyr_2209 at 2567.745 kb on - strand, within Psyr_2209 at 2567.818 kb on - strand, within Psyr_2209 at 2568.364 kb on - strand, within Psyr_2210 at 2568.537 kb on + strand, within Psyr_2210 at 2568.537 kb on + strand, within Psyr_2210 at 2568.537 kb on + strand, within Psyr_2210 at 2568.538 kb on - strand at 2568.977 kb on - strand, within Psyr_2210 at 2569.012 kb on + strand, within Psyr_2210 at 2569.013 kb on - strand, within Psyr_2210
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 2,566,210 + Psyr_2208 0.41 -0.9 2,566,214 + Psyr_2208 0.41 +1.4 2,566,214 + Psyr_2208 0.41 -0.1 2,566,229 + Psyr_2208 0.42 -2.2 2,566,230 - Psyr_2208 0.42 -0.0 2,566,643 + Psyr_2208 0.69 +0.4 2,566,643 + Psyr_2208 0.69 +1.4 2,566,644 - Psyr_2208 0.69 +0.3 2,566,664 + Psyr_2208 0.71 +0.2 2,566,665 - Psyr_2208 0.71 +0.4 2,566,739 + Psyr_2208 0.75 +0.2 2,566,767 - Psyr_2208 0.77 -0.9 2,566,811 + Psyr_2208 0.80 -0.4 2,566,846 - Psyr_2208 0.82 -0.8 2,566,907 + Psyr_2208 0.86 +0.2 2,566,907 + Psyr_2208 0.86 -0.8 2,566,908 - Psyr_2208 0.86 +1.1 2,566,908 - Psyr_2208 0.86 +0.9 2,566,908 - Psyr_2208 0.86 -0.9 2,567,073 - +0.8 2,567,302 - Psyr_2209 0.13 -0.2 2,567,481 + Psyr_2209 0.31 -0.3 2,567,482 - Psyr_2209 0.31 +0.0 2,567,494 - Psyr_2209 0.33 -2.5 2,567,691 + Psyr_2209 0.53 -1.2 2,567,745 - Psyr_2209 0.58 -1.1 2,567,745 - Psyr_2209 0.58 -0.6 2,567,818 - Psyr_2209 0.66 -2.3 2,568,364 - Psyr_2210 0.14 -0.6 2,568,537 + Psyr_2210 0.32 -0.0 2,568,537 + Psyr_2210 0.32 +0.7 2,568,537 + Psyr_2210 0.32 +0.6 2,568,538 - -0.2 2,568,977 - Psyr_2210 0.78 -1.3 2,569,012 + Psyr_2210 0.82 -0.4 2,569,013 - Psyr_2210 0.82 -0.8
Or see this region's nucleotide sequence