Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2157 and Psyr_2158 are separated by 22 nucleotides Psyr_2158 and Psyr_2159 are separated by 309 nucleotides Psyr_2159 and Psyr_2160 are separated by 66 nucleotides
Psyr_2157: Psyr_2157 - Inosine/uridine-preferring nucleoside hydrolase, at 2,512,612 to 2,513,637
_2157
Psyr_2158: Psyr_2158 - conserved hypothetical protein, at 2,513,660 to 2,513,854
_2158
Psyr_2159: Psyr_2159 - lipoprotein, putative, at 2,514,164 to 2,514,505
_2159
Psyr_2160: Psyr_2160 - cold-shock DNA-binding protein family, at 2,514,572 to 2,514,784
_2160
Position (kb)
2513
2514 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2512.754 kb on + strand, within Psyr_2157 at 2512.755 kb on - strand, within Psyr_2157 at 2512.889 kb on - strand, within Psyr_2157 at 2512.889 kb on - strand, within Psyr_2157 at 2512.927 kb on + strand, within Psyr_2157 at 2512.928 kb on - strand, within Psyr_2157 at 2512.928 kb on - strand, within Psyr_2157 at 2513.053 kb on - strand, within Psyr_2157 at 2513.178 kb on + strand, within Psyr_2157 at 2513.260 kb on + strand, within Psyr_2157 at 2513.260 kb on + strand, within Psyr_2157 at 2513.260 kb on + strand, within Psyr_2157 at 2513.260 kb on + strand, within Psyr_2157 at 2513.260 kb on + strand, within Psyr_2157 at 2513.260 kb on + strand, within Psyr_2157 at 2513.260 kb on + strand, within Psyr_2157 at 2513.260 kb on + strand, within Psyr_2157 at 2513.260 kb on + strand, within Psyr_2157 at 2513.260 kb on + strand, within Psyr_2157 at 2513.260 kb on + strand, within Psyr_2157 at 2513.261 kb on - strand, within Psyr_2157 at 2513.261 kb on - strand, within Psyr_2157 at 2513.261 kb on - strand, within Psyr_2157 at 2513.261 kb on - strand, within Psyr_2157 at 2513.261 kb on - strand, within Psyr_2157 at 2513.261 kb on - strand, within Psyr_2157 at 2513.261 kb on - strand, within Psyr_2157 at 2513.261 kb on - strand, within Psyr_2157 at 2513.261 kb on - strand, within Psyr_2157 at 2513.371 kb on + strand, within Psyr_2157 at 2513.371 kb on + strand, within Psyr_2157 at 2513.371 kb on + strand, within Psyr_2157 at 2513.371 kb on + strand, within Psyr_2157 at 2513.372 kb on - strand, within Psyr_2157 at 2513.372 kb on - strand, within Psyr_2157 at 2513.536 kb on + strand at 2513.564 kb on + strand at 2513.565 kb on - strand at 2513.565 kb on - strand at 2513.565 kb on - strand at 2513.635 kb on + strand at 2513.635 kb on + strand at 2513.635 kb on + strand at 2513.635 kb on + strand at 2513.636 kb on - strand at 2513.636 kb on - strand at 2513.636 kb on - strand at 2513.639 kb on + strand at 2513.639 kb on + strand at 2513.640 kb on - strand at 2513.640 kb on - strand at 2513.640 kb on - strand at 2513.640 kb on - strand at 2513.686 kb on - strand, within Psyr_2158 at 2513.924 kb on - strand at 2513.957 kb on + strand at 2513.957 kb on + strand at 2513.958 kb on - strand at 2513.958 kb on - strand at 2513.958 kb on - strand at 2513.958 kb on - strand at 2513.961 kb on + strand at 2513.961 kb on + strand at 2513.961 kb on + strand at 2513.962 kb on - strand at 2513.962 kb on - strand at 2513.962 kb on - strand at 2514.163 kb on + strand at 2514.163 kb on + strand at 2514.164 kb on - strand at 2514.164 kb on - strand at 2514.193 kb on + strand at 2514.242 kb on - strand, within Psyr_2159 at 2514.616 kb on + strand, within Psyr_2160 at 2514.616 kb on + strand, within Psyr_2160 at 2514.689 kb on + strand, within Psyr_2160 at 2514.725 kb on - strand, within Psyr_2160 at 2514.759 kb on - strand, within Psyr_2160 at 2514.759 kb on - strand, within Psyr_2160 at 2514.789 kb on + strand at 2514.789 kb on + strand at 2514.790 kb on - strand at 2514.790 kb on - strand at 2514.790 kb on - strand at 2514.790 kb on - strand at 2514.849 kb on - strand at 2514.853 kb on + strand at 2514.854 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 2,512,754 + Psyr_2157 0.14 +0.6 2,512,755 - Psyr_2157 0.14 +1.4 2,512,889 - Psyr_2157 0.27 -0.9 2,512,889 - Psyr_2157 0.27 -0.3 2,512,927 + Psyr_2157 0.31 -2.7 2,512,928 - Psyr_2157 0.31 +1.0 2,512,928 - Psyr_2157 0.31 -1.8 2,513,053 - Psyr_2157 0.43 +0.0 2,513,178 + Psyr_2157 0.55 +0.5 2,513,260 + Psyr_2157 0.63 +0.3 2,513,260 + Psyr_2157 0.63 +0.2 2,513,260 + Psyr_2157 0.63 -1.3 2,513,260 + Psyr_2157 0.63 -0.0 2,513,260 + Psyr_2157 0.63 -0.4 2,513,260 + Psyr_2157 0.63 +1.7 2,513,260 + Psyr_2157 0.63 -2.3 2,513,260 + Psyr_2157 0.63 -0.5 2,513,260 + Psyr_2157 0.63 -0.6 2,513,260 + Psyr_2157 0.63 -0.9 2,513,260 + Psyr_2157 0.63 -0.2 2,513,261 - Psyr_2157 0.63 -0.0 2,513,261 - Psyr_2157 0.63 +1.4 2,513,261 - Psyr_2157 0.63 -0.4 2,513,261 - Psyr_2157 0.63 +4.4 2,513,261 - Psyr_2157 0.63 -1.7 2,513,261 - Psyr_2157 0.63 -0.6 2,513,261 - Psyr_2157 0.63 -1.7 2,513,261 - Psyr_2157 0.63 -0.2 2,513,261 - Psyr_2157 0.63 +0.5 2,513,371 + Psyr_2157 0.74 +0.2 2,513,371 + Psyr_2157 0.74 +2.3 2,513,371 + Psyr_2157 0.74 +1.9 2,513,371 + Psyr_2157 0.74 -1.6 2,513,372 - Psyr_2157 0.74 +1.0 2,513,372 - Psyr_2157 0.74 +0.8 2,513,536 + -0.9 2,513,564 + +0.3 2,513,565 - -1.4 2,513,565 - +2.0 2,513,565 - -0.6 2,513,635 + -0.3 2,513,635 + -0.8 2,513,635 + +0.6 2,513,635 + -0.2 2,513,636 - +3.0 2,513,636 - -0.2 2,513,636 - -1.9 2,513,639 + +1.0 2,513,639 + +1.0 2,513,640 - -1.4 2,513,640 - +0.1 2,513,640 - -0.4 2,513,640 - -0.2 2,513,686 - Psyr_2158 0.13 +0.0 2,513,924 - -0.4 2,513,957 + +0.0 2,513,957 + +0.9 2,513,958 - +1.1 2,513,958 - -0.7 2,513,958 - -0.6 2,513,958 - +0.1 2,513,961 + +0.5 2,513,961 + -1.0 2,513,961 + +0.5 2,513,962 - -0.9 2,513,962 - +0.6 2,513,962 - -0.4 2,514,163 + +2.6 2,514,163 + -1.4 2,514,164 - +1.1 2,514,164 - -0.2 2,514,193 + +1.1 2,514,242 - Psyr_2159 0.23 -1.3 2,514,616 + Psyr_2160 0.21 +0.2 2,514,616 + Psyr_2160 0.21 -0.2 2,514,689 + Psyr_2160 0.55 +1.4 2,514,725 - Psyr_2160 0.72 +0.6 2,514,759 - Psyr_2160 0.88 -1.1 2,514,759 - Psyr_2160 0.88 +1.0 2,514,789 + -0.0 2,514,789 + +1.4 2,514,790 - +1.9 2,514,790 - +0.6 2,514,790 - +0.1 2,514,790 - +0.2 2,514,849 - +0.8 2,514,853 + -2.6 2,514,854 - +1.0
Or see this region's nucleotide sequence