Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2095 and Psyr_2096 are separated by 82 nucleotides Psyr_2096 and Psyr_2097 are separated by 105 nucleotides Psyr_2097 and Psyr_2098 are separated by 87 nucleotides Psyr_2098 and Psyr_2099 are separated by 10 nucleotides
Psyr_2095: Psyr_2095 - Conserved TM helix, at 2,443,112 to 2,443,936
_2095
Psyr_2096: Psyr_2096 - Sigma-70 region 2, at 2,444,019 to 2,444,609
_2096
Psyr_2097: Psyr_2097 - Thrombospondin type 3 repeat:OmpA/MotB:OmpF, at 2,444,715 to 2,445,749
_2097
Psyr_2098: Psyr_2098 - uroporphyrinogen-III C-methyltransferase, at 2,445,837 to 2,446,598
_2098
Psyr_2099: Psyr_2099 - assimilatory nitrate reductase (NADH) alpha subunit apoprotein, at 2,446,609 to 2,449,329
_2099
Position (kb)
2444
2445
2446 Strain fitness (log2 ratio)
-2
-1
0
1 at 2443.772 kb on + strand, within Psyr_2095 at 2443.772 kb on + strand at 2443.812 kb on + strand, within Psyr_2095 at 2443.923 kb on + strand at 2443.950 kb on + strand at 2443.955 kb on + strand at 2443.955 kb on + strand at 2444.049 kb on + strand at 2444.336 kb on + strand, within Psyr_2096 at 2444.443 kb on - strand, within Psyr_2096 at 2444.607 kb on + strand at 2444.607 kb on + strand at 2444.621 kb on + strand at 2444.621 kb on + strand at 2444.621 kb on + strand at 2445.462 kb on + strand, within Psyr_2097 at 2445.558 kb on + strand, within Psyr_2097 at 2445.618 kb on + strand, within Psyr_2097 at 2445.747 kb on + strand at 2445.748 kb on - strand at 2445.748 kb on - strand at 2445.895 kb on + strand at 2445.896 kb on - strand at 2446.099 kb on + strand, within Psyr_2098 at 2446.099 kb on + strand, within Psyr_2098 at 2446.099 kb on + strand, within Psyr_2098 at 2446.100 kb on - strand, within Psyr_2098 at 2446.100 kb on - strand, within Psyr_2098 at 2446.194 kb on + strand, within Psyr_2098 at 2446.194 kb on + strand, within Psyr_2098 at 2446.194 kb on + strand, within Psyr_2098 at 2446.195 kb on - strand, within Psyr_2098 at 2446.195 kb on - strand, within Psyr_2098 at 2446.197 kb on - strand, within Psyr_2098 at 2446.251 kb on - strand, within Psyr_2098 at 2446.251 kb on - strand, within Psyr_2098 at 2446.376 kb on - strand, within Psyr_2098 at 2446.584 kb on + strand at 2446.584 kb on + strand at 2446.584 kb on + strand at 2446.584 kb on + strand at 2446.585 kb on - strand at 2446.585 kb on - strand at 2446.585 kb on - strand at 2446.645 kb on + strand at 2446.646 kb on - strand at 2446.646 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 2,443,772 + Psyr_2095 0.80 -1.0 2,443,772 + -1.0 2,443,812 + Psyr_2095 0.85 +1.5 2,443,923 + +1.1 2,443,950 + -0.5 2,443,955 + -0.9 2,443,955 + -1.5 2,444,049 + +0.4 2,444,336 + Psyr_2096 0.54 -1.0 2,444,443 - Psyr_2096 0.72 -1.0 2,444,607 + +0.4 2,444,607 + -0.0 2,444,621 + +0.2 2,444,621 + -1.6 2,444,621 + +0.6 2,445,462 + Psyr_2097 0.72 -1.0 2,445,558 + Psyr_2097 0.81 +1.0 2,445,618 + Psyr_2097 0.87 +0.2 2,445,747 + -0.8 2,445,748 - -1.0 2,445,748 - -1.0 2,445,895 + +1.0 2,445,896 - +0.2 2,446,099 + Psyr_2098 0.34 -0.4 2,446,099 + Psyr_2098 0.34 +0.1 2,446,099 + Psyr_2098 0.34 -1.6 2,446,100 - Psyr_2098 0.35 -0.8 2,446,100 - Psyr_2098 0.35 +0.9 2,446,194 + Psyr_2098 0.47 -0.6 2,446,194 + Psyr_2098 0.47 -0.2 2,446,194 + Psyr_2098 0.47 -1.8 2,446,195 - Psyr_2098 0.47 -0.8 2,446,195 - Psyr_2098 0.47 +1.1 2,446,197 - Psyr_2098 0.47 +0.4 2,446,251 - Psyr_2098 0.54 +0.2 2,446,251 - Psyr_2098 0.54 -0.1 2,446,376 - Psyr_2098 0.71 -0.5 2,446,584 + -2.1 2,446,584 + +0.8 2,446,584 + +0.4 2,446,584 + +0.4 2,446,585 - +0.1 2,446,585 - -0.1 2,446,585 - -0.7 2,446,645 + -1.6 2,446,646 - +0.4 2,446,646 - +0.2
Or see this region's nucleotide sequence