Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_2012

Experiment: KB with Aristolochic Acid 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_2011 and Psyr_2012 are separated by 208 nucleotidesPsyr_2012 and Psyr_2013 overlap by 1 nucleotidesPsyr_2013 and Psyr_2014 are separated by 98 nucleotides Psyr_2011: Psyr_2011 - dihydrolipoamide dehydrogenase, at 2,333,942 to 2,335,378 _2011 Psyr_2012: Psyr_2012 - succinyl-CoA synthetase (ADP-forming) beta subunit, at 2,335,587 to 2,336,753 _2012 Psyr_2013: Psyr_2013 - succinyl-CoA synthetase (ADP-forming) alpha subunit, at 2,336,753 to 2,337,634 _2013 Psyr_2014: Psyr_2014 - conserved hypothetical protein, at 2,337,733 to 2,338,308 _2014 Position (kb) 2335 2336 2337Strain fitness (log2 ratio) -1 0 1at 2337.734 kb on + strandat 2337.734 kb on + strandat 2337.734 kb on + strandat 2337.735 kb on - strandat 2337.735 kb on - strandat 2337.744 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM
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2,337,734 + +0.8
2,337,734 + +0.8
2,337,734 + +1.4
2,337,735 - +0.3
2,337,735 - -0.4
2,337,744 + +1.0

Or see this region's nucleotide sequence