Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1948 and Psyr_1949 are separated by 0 nucleotides Psyr_1949 and Psyr_1950 overlap by 1 nucleotides Psyr_1950 and Psyr_1951 overlap by 4 nucleotides Psyr_1951 and Psyr_1952 are separated by 35 nucleotides
Psyr_1948: Psyr_1948 - ABC transporter, periplasmic substrate-binding protein, putative, at 2,233,389 to 2,234,306
_1948
Psyr_1949: Psyr_1949 - ABC-3, at 2,234,307 to 2,235,206
_1949
Psyr_1950: Psyr_1950 - ABC transporter, at 2,235,206 to 2,235,970
_1950
Psyr_1951: Psyr_1951 - Periplasmic solute binding protein, at 2,235,967 to 2,236,899
_1951
Psyr_1952: Psyr_1952 - hypothetical protein, at 2,236,935 to 2,237,501
_1952
Position (kb)
2235
2236 Strain fitness (log2 ratio)
-2
-1
0
1 at 2234.291 kb on + strand at 2234.291 kb on + strand at 2234.292 kb on - strand at 2234.292 kb on - strand at 2234.295 kb on + strand at 2234.295 kb on + strand at 2234.309 kb on - strand at 2234.615 kb on - strand, within Psyr_1949 at 2234.689 kb on + strand, within Psyr_1949 at 2234.854 kb on - strand, within Psyr_1949 at 2234.912 kb on - strand, within Psyr_1949 at 2234.959 kb on + strand, within Psyr_1949 at 2234.971 kb on + strand, within Psyr_1949 at 2234.972 kb on - strand, within Psyr_1949 at 2235.721 kb on - strand, within Psyr_1950 at 2236.056 kb on - strand at 2236.056 kb on - strand at 2236.115 kb on + strand, within Psyr_1951 at 2236.116 kb on - strand, within Psyr_1951 at 2236.116 kb on - strand, within Psyr_1951 at 2236.392 kb on - strand, within Psyr_1951 at 2236.633 kb on + strand, within Psyr_1951 at 2236.686 kb on - strand, within Psyr_1951 at 2236.757 kb on + strand, within Psyr_1951 at 2236.757 kb on + strand, within Psyr_1951 at 2236.757 kb on + strand, within Psyr_1951 at 2236.757 kb on + strand, within Psyr_1951 at 2236.758 kb on - strand, within Psyr_1951 at 2236.763 kb on + strand, within Psyr_1951 at 2236.763 kb on + strand, within Psyr_1951 at 2236.763 kb on + strand, within Psyr_1951 at 2236.763 kb on + strand, within Psyr_1951 at 2236.763 kb on + strand, within Psyr_1951 at 2236.764 kb on - strand, within Psyr_1951 at 2236.766 kb on + strand, within Psyr_1951 at 2236.780 kb on - strand, within Psyr_1951 at 2236.893 kb on + strand at 2236.893 kb on + strand at 2236.894 kb on - strand at 2236.894 kb on - strand at 2236.894 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 2,234,291 + -2.1 2,234,291 + +1.4 2,234,292 - -0.1 2,234,292 - +0.3 2,234,295 + -0.9 2,234,295 + +1.4 2,234,309 - -1.0 2,234,615 - Psyr_1949 0.34 -0.5 2,234,689 + Psyr_1949 0.42 +1.0 2,234,854 - Psyr_1949 0.61 +0.5 2,234,912 - Psyr_1949 0.67 +0.5 2,234,959 + Psyr_1949 0.72 -0.2 2,234,971 + Psyr_1949 0.74 +0.0 2,234,972 - Psyr_1949 0.74 -0.4 2,235,721 - Psyr_1950 0.67 -0.3 2,236,056 - -0.2 2,236,056 - +0.9 2,236,115 + Psyr_1951 0.16 -1.3 2,236,116 - Psyr_1951 0.16 -0.6 2,236,116 - Psyr_1951 0.16 +1.7 2,236,392 - Psyr_1951 0.46 -1.2 2,236,633 + Psyr_1951 0.71 +0.5 2,236,686 - Psyr_1951 0.77 +0.2 2,236,757 + Psyr_1951 0.85 -0.1 2,236,757 + Psyr_1951 0.85 +0.6 2,236,757 + Psyr_1951 0.85 -0.0 2,236,757 + Psyr_1951 0.85 -0.1 2,236,758 - Psyr_1951 0.85 -0.7 2,236,763 + Psyr_1951 0.85 -0.1 2,236,763 + Psyr_1951 0.85 +0.1 2,236,763 + Psyr_1951 0.85 +0.8 2,236,763 + Psyr_1951 0.85 -0.1 2,236,763 + Psyr_1951 0.85 -0.7 2,236,764 - Psyr_1951 0.85 -0.3 2,236,766 + Psyr_1951 0.86 -1.0 2,236,780 - Psyr_1951 0.87 +1.2 2,236,893 + +0.1 2,236,893 + +1.2 2,236,894 - -0.3 2,236,894 - -1.0 2,236,894 - +0.4
Or see this region's nucleotide sequence