Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1907 and Psyr_1908 are separated by 149 nucleotides Psyr_1908 and Psyr_1909 are separated by 103 nucleotides Psyr_1909 and Psyr_1910 are separated by 198 nucleotides Psyr_1910 and Psyr_1911 are separated by 88 nucleotides
Psyr_1907: Psyr_1907 - GTP cyclohydrolase I, at 2,182,616 to 2,183,530
_1907
Psyr_1908: Psyr_1908 - conserved hypothetical protein, at 2,183,680 to 2,183,949
_1908
Psyr_1909: Psyr_1909 - conserved hypothetical protein, at 2,184,053 to 2,184,661
_1909
Psyr_1910: Psyr_1910 - VacJ-like lipoprotein, at 2,184,860 to 2,185,561
_1910
Psyr_1911: Psyr_1911 - conserved hypothetical protein, at 2,185,650 to 2,185,949
_1911
Position (kb)
2184
2185 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2183.283 kb on + strand, within Psyr_1907 at 2183.334 kb on - strand, within Psyr_1907 at 2183.528 kb on + strand at 2183.600 kb on - strand at 2183.610 kb on - strand at 2183.968 kb on + strand at 2183.968 kb on + strand at 2183.968 kb on + strand at 2183.969 kb on - strand at 2183.969 kb on - strand at 2183.969 kb on - strand at 2183.999 kb on + strand at 2183.999 kb on + strand at 2184.000 kb on - strand at 2184.023 kb on + strand at 2184.024 kb on - strand at 2184.024 kb on - strand at 2184.051 kb on + strand at 2184.052 kb on - strand at 2184.052 kb on - strand at 2184.052 kb on - strand at 2184.052 kb on - strand at 2184.052 kb on - strand at 2184.052 kb on - strand at 2184.317 kb on + strand, within Psyr_1909 at 2184.767 kb on + strand at 2184.786 kb on + strand at 2184.786 kb on + strand at 2184.787 kb on - strand at 2184.787 kb on - strand at 2184.788 kb on + strand at 2184.788 kb on + strand at 2184.788 kb on + strand at 2184.789 kb on - strand at 2184.789 kb on - strand at 2184.789 kb on - strand at 2184.789 kb on - strand at 2184.800 kb on + strand at 2184.829 kb on + strand at 2184.859 kb on + strand at 2184.859 kb on + strand at 2184.860 kb on - strand at 2184.860 kb on - strand at 2185.017 kb on - strand, within Psyr_1910 at 2185.017 kb on - strand, within Psyr_1910 at 2185.044 kb on - strand, within Psyr_1910 at 2185.049 kb on + strand, within Psyr_1910 at 2185.049 kb on + strand, within Psyr_1910 at 2185.049 kb on + strand, within Psyr_1910 at 2185.049 kb on + strand, within Psyr_1910 at 2185.049 kb on + strand, within Psyr_1910 at 2185.050 kb on - strand, within Psyr_1910 at 2185.050 kb on - strand, within Psyr_1910 at 2185.050 kb on - strand, within Psyr_1910 at 2185.050 kb on - strand, within Psyr_1910 at 2185.050 kb on - strand, within Psyr_1910 at 2185.050 kb on - strand, within Psyr_1910 at 2185.332 kb on + strand, within Psyr_1910 at 2185.332 kb on + strand, within Psyr_1910 at 2185.332 kb on + strand, within Psyr_1910 at 2185.332 kb on + strand, within Psyr_1910 at 2185.332 kb on + strand, within Psyr_1910 at 2185.333 kb on - strand, within Psyr_1910 at 2185.333 kb on - strand, within Psyr_1910 at 2185.364 kb on + strand, within Psyr_1910 at 2185.365 kb on - strand, within Psyr_1910 at 2185.484 kb on + strand, within Psyr_1910 at 2185.506 kb on - strand at 2185.567 kb on - strand at 2185.612 kb on + strand at 2185.613 kb on - strand at 2185.616 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 2,183,283 + Psyr_1907 0.73 -1.2 2,183,334 - Psyr_1907 0.78 +0.4 2,183,528 + +0.2 2,183,600 - +0.8 2,183,610 - -2.3 2,183,968 + -0.4 2,183,968 + +1.0 2,183,968 + +0.7 2,183,969 - +0.4 2,183,969 - -0.3 2,183,969 - -2.2 2,183,999 + -0.4 2,183,999 + +0.4 2,184,000 - +0.4 2,184,023 + -0.6 2,184,024 - +1.4 2,184,024 - -1.5 2,184,051 + -1.3 2,184,052 - -2.0 2,184,052 - -1.5 2,184,052 - -1.8 2,184,052 - +0.9 2,184,052 - -0.2 2,184,052 - +0.4 2,184,317 + Psyr_1909 0.43 +0.4 2,184,767 + -0.6 2,184,786 + +1.4 2,184,786 + -0.8 2,184,787 - -1.6 2,184,787 - -1.2 2,184,788 + +1.2 2,184,788 + -0.3 2,184,788 + -0.9 2,184,789 - +0.8 2,184,789 - +0.9 2,184,789 - +0.4 2,184,789 - +0.4 2,184,800 + -0.1 2,184,829 + +1.4 2,184,859 + +2.1 2,184,859 + -0.4 2,184,860 - +0.6 2,184,860 - -1.0 2,185,017 - Psyr_1910 0.22 -2.0 2,185,017 - Psyr_1910 0.22 +0.0 2,185,044 - Psyr_1910 0.26 +1.4 2,185,049 + Psyr_1910 0.27 -1.8 2,185,049 + Psyr_1910 0.27 +0.5 2,185,049 + Psyr_1910 0.27 -0.2 2,185,049 + Psyr_1910 0.27 -0.8 2,185,049 + Psyr_1910 0.27 -0.2 2,185,050 - Psyr_1910 0.27 -1.8 2,185,050 - Psyr_1910 0.27 -1.6 2,185,050 - Psyr_1910 0.27 -0.2 2,185,050 - Psyr_1910 0.27 +0.1 2,185,050 - Psyr_1910 0.27 +0.8 2,185,050 - Psyr_1910 0.27 -0.2 2,185,332 + Psyr_1910 0.67 -0.3 2,185,332 + Psyr_1910 0.67 -0.6 2,185,332 + Psyr_1910 0.67 +0.4 2,185,332 + Psyr_1910 0.67 +1.7 2,185,332 + Psyr_1910 0.67 -1.8 2,185,333 - Psyr_1910 0.67 -1.9 2,185,333 - Psyr_1910 0.67 -0.1 2,185,364 + Psyr_1910 0.72 -0.4 2,185,365 - Psyr_1910 0.72 +1.7 2,185,484 + Psyr_1910 0.89 +0.4 2,185,506 - -0.6 2,185,567 - +0.7 2,185,612 + -1.0 2,185,613 - -0.4 2,185,616 - -1.2
Or see this region's nucleotide sequence