Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1865 and Psyr_1866 are separated by 190 nucleotides Psyr_1866 and Psyr_1867 are separated by 247 nucleotides Psyr_1867 and Psyr_1868 overlap by 40 nucleotides
Psyr_1865: Psyr_1865 - Sulfatase, at 2,140,673 to 2,142,760
_1865
Psyr_1866: Psyr_1866 - conserved hypothetical protein, at 2,142,951 to 2,143,187
_1866
Psyr_1867: Psyr_1867 - conserved hypothetical protein, at 2,143,435 to 2,143,776
_1867
Psyr_1868: Psyr_1868 - hypothetical protein, at 2,143,737 to 2,144,072
_1868
Position (kb)
2142
2143
2144 Strain fitness (log2 ratio)
-1
0
1
2 at 2142.080 kb on + strand, within Psyr_1865 at 2142.081 kb on - strand, within Psyr_1865 at 2142.081 kb on - strand, within Psyr_1865 at 2142.081 kb on - strand, within Psyr_1865 at 2142.081 kb on - strand, within Psyr_1865 at 2142.081 kb on - strand, within Psyr_1865 at 2142.081 kb on - strand, within Psyr_1865 at 2142.081 kb on - strand, within Psyr_1865 at 2142.081 kb on - strand, within Psyr_1865 at 2142.245 kb on + strand, within Psyr_1865 at 2142.245 kb on + strand, within Psyr_1865 at 2142.246 kb on - strand, within Psyr_1865 at 2142.246 kb on - strand at 2142.246 kb on - strand, within Psyr_1865 at 2142.248 kb on + strand, within Psyr_1865 at 2142.248 kb on + strand, within Psyr_1865 at 2142.248 kb on + strand, within Psyr_1865 at 2142.248 kb on + strand, within Psyr_1865 at 2142.249 kb on - strand, within Psyr_1865 at 2142.249 kb on - strand, within Psyr_1865 at 2142.249 kb on - strand, within Psyr_1865 at 2142.391 kb on + strand, within Psyr_1865 at 2142.608 kb on + strand at 2142.608 kb on + strand at 2142.609 kb on - strand at 2142.609 kb on - strand at 2142.609 kb on - strand at 2142.735 kb on + strand at 2142.735 kb on + strand at 2142.735 kb on + strand at 2142.736 kb on - strand at 2142.736 kb on - strand at 2142.736 kb on - strand at 2142.915 kb on + strand at 2142.916 kb on - strand at 2142.916 kb on - strand at 2142.972 kb on + strand at 2142.972 kb on + strand at 2142.973 kb on - strand at 2143.125 kb on - strand, within Psyr_1866 at 2143.148 kb on + strand, within Psyr_1866 at 2143.149 kb on - strand, within Psyr_1866 at 2143.173 kb on - strand at 2143.241 kb on + strand at 2143.247 kb on + strand at 2143.248 kb on - strand at 2143.323 kb on + strand at 2143.323 kb on + strand at 2143.323 kb on + strand at 2143.324 kb on - strand at 2143.324 kb on - strand at 2143.324 kb on - strand at 2143.324 kb on - strand at 2143.324 kb on - strand at 2143.324 kb on - strand at 2143.350 kb on - strand at 2143.407 kb on + strand at 2143.407 kb on + strand at 2143.408 kb on - strand at 2143.408 kb on - strand at 2143.408 kb on - strand at 2143.411 kb on + strand at 2143.411 kb on + strand at 2143.411 kb on + strand at 2143.411 kb on + strand at 2143.412 kb on - strand at 2143.412 kb on - strand at 2143.412 kb on - strand at 2143.412 kb on - strand at 2143.412 kb on - strand at 2143.412 kb on - strand at 2143.421 kb on + strand at 2143.421 kb on + strand at 2143.422 kb on - strand at 2143.422 kb on - strand at 2143.447 kb on + strand at 2143.467 kb on + strand at 2143.467 kb on + strand at 2143.468 kb on - strand at 2143.468 kb on - strand at 2143.472 kb on + strand, within Psyr_1867 at 2143.472 kb on + strand, within Psyr_1867 at 2143.516 kb on + strand, within Psyr_1867 at 2143.517 kb on - strand, within Psyr_1867 at 2143.613 kb on + strand, within Psyr_1867 at 2143.614 kb on - strand, within Psyr_1867 at 2143.614 kb on - strand, within Psyr_1867 at 2143.614 kb on - strand, within Psyr_1867 at 2143.614 kb on - strand, within Psyr_1867 at 2143.614 kb on - strand, within Psyr_1867 at 2143.657 kb on + strand, within Psyr_1867 at 2143.658 kb on - strand, within Psyr_1867 at 2143.658 kb on - strand, within Psyr_1867 at 2143.658 kb on - strand, within Psyr_1867 at 2143.658 kb on - strand, within Psyr_1867 at 2143.666 kb on + strand, within Psyr_1867 at 2143.666 kb on + strand, within Psyr_1867 at 2143.667 kb on - strand, within Psyr_1867 at 2143.667 kb on - strand, within Psyr_1867 at 2143.716 kb on + strand, within Psyr_1867 at 2143.717 kb on - strand, within Psyr_1867 at 2143.798 kb on + strand, within Psyr_1868 at 2143.798 kb on + strand, within Psyr_1868 at 2143.799 kb on - strand at 2143.799 kb on - strand, within Psyr_1868 at 2143.917 kb on - strand, within Psyr_1868 at 2143.952 kb on + strand, within Psyr_1868 at 2144.017 kb on + strand, within Psyr_1868 at 2144.018 kb on - strand, within Psyr_1868 at 2144.018 kb on - strand, within Psyr_1868 at 2144.018 kb on - strand, within Psyr_1868 at 2144.018 kb on - strand, within Psyr_1868 at 2144.018 kb on - strand, within Psyr_1868 at 2144.018 kb on - strand, within Psyr_1868 at 2144.046 kb on + strand at 2144.046 kb on + strand at 2144.057 kb on + strand at 2144.057 kb on + strand at 2144.058 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 2,142,080 + Psyr_1865 0.67 +0.2 2,142,081 - Psyr_1865 0.67 +0.0 2,142,081 - Psyr_1865 0.67 +0.4 2,142,081 - Psyr_1865 0.67 +0.6 2,142,081 - Psyr_1865 0.67 +0.1 2,142,081 - Psyr_1865 0.67 -0.8 2,142,081 - Psyr_1865 0.67 +0.1 2,142,081 - Psyr_1865 0.67 -1.6 2,142,081 - Psyr_1865 0.67 +0.5 2,142,245 + Psyr_1865 0.75 +0.4 2,142,245 + Psyr_1865 0.75 +0.4 2,142,246 - Psyr_1865 0.75 -0.5 2,142,246 - +2.1 2,142,246 - Psyr_1865 0.75 -1.0 2,142,248 + Psyr_1865 0.75 -0.2 2,142,248 + Psyr_1865 0.75 -0.0 2,142,248 + Psyr_1865 0.75 +0.3 2,142,248 + Psyr_1865 0.75 +0.6 2,142,249 - Psyr_1865 0.75 +0.6 2,142,249 - Psyr_1865 0.75 +0.4 2,142,249 - Psyr_1865 0.75 +0.5 2,142,391 + Psyr_1865 0.82 +0.1 2,142,608 + -0.0 2,142,608 + -0.3 2,142,609 - +0.6 2,142,609 - -0.8 2,142,609 - -0.1 2,142,735 + -0.0 2,142,735 + -0.3 2,142,735 + +1.6 2,142,736 - +0.8 2,142,736 - +0.4 2,142,736 - +0.5 2,142,915 + +1.3 2,142,916 - -0.6 2,142,916 - +0.5 2,142,972 + -0.3 2,142,972 + +0.8 2,142,973 - +0.4 2,143,125 - Psyr_1866 0.73 +0.7 2,143,148 + Psyr_1866 0.83 +0.1 2,143,149 - Psyr_1866 0.84 -1.2 2,143,173 - -0.2 2,143,241 + +0.5 2,143,247 + +1.4 2,143,248 - -0.1 2,143,323 + +0.5 2,143,323 + -1.1 2,143,323 + +0.1 2,143,324 - +2.1 2,143,324 - +0.9 2,143,324 - +0.4 2,143,324 - +0.0 2,143,324 - -0.1 2,143,324 - -1.1 2,143,350 - -0.3 2,143,407 + -0.0 2,143,407 + +0.1 2,143,408 - +1.0 2,143,408 - +0.4 2,143,408 - -0.7 2,143,411 + -0.8 2,143,411 + +1.2 2,143,411 + +1.0 2,143,411 + -0.1 2,143,412 - -1.0 2,143,412 - +0.5 2,143,412 - -0.3 2,143,412 - +0.4 2,143,412 - +1.4 2,143,412 - +0.1 2,143,421 + -0.0 2,143,421 + -1.0 2,143,422 - +0.5 2,143,422 - -0.8 2,143,447 + +0.0 2,143,467 + -0.1 2,143,467 + -0.1 2,143,468 - +0.5 2,143,468 - +1.6 2,143,472 + Psyr_1867 0.11 -1.5 2,143,472 + Psyr_1867 0.11 -0.1 2,143,516 + Psyr_1867 0.24 +0.4 2,143,517 - Psyr_1867 0.24 +1.4 2,143,613 + Psyr_1867 0.52 -0.1 2,143,614 - Psyr_1867 0.52 +2.0 2,143,614 - Psyr_1867 0.52 +0.1 2,143,614 - Psyr_1867 0.52 -0.9 2,143,614 - Psyr_1867 0.52 +0.6 2,143,614 - Psyr_1867 0.52 -0.6 2,143,657 + Psyr_1867 0.65 -1.3 2,143,658 - Psyr_1867 0.65 +0.2 2,143,658 - Psyr_1867 0.65 -1.3 2,143,658 - Psyr_1867 0.65 -0.5 2,143,658 - Psyr_1867 0.65 +0.4 2,143,666 + Psyr_1867 0.68 -0.4 2,143,666 + Psyr_1867 0.68 -0.6 2,143,667 - Psyr_1867 0.68 +1.9 2,143,667 - Psyr_1867 0.68 +1.1 2,143,716 + Psyr_1867 0.82 +0.5 2,143,717 - Psyr_1867 0.82 -1.1 2,143,798 + Psyr_1868 0.18 -0.2 2,143,798 + Psyr_1868 0.18 -0.2 2,143,799 - -0.6 2,143,799 - Psyr_1868 0.18 -0.2 2,143,917 - Psyr_1868 0.54 +0.0 2,143,952 + Psyr_1868 0.64 +0.1 2,144,017 + Psyr_1868 0.83 +1.1 2,144,018 - Psyr_1868 0.84 -0.2 2,144,018 - Psyr_1868 0.84 +0.5 2,144,018 - Psyr_1868 0.84 +1.7 2,144,018 - Psyr_1868 0.84 +0.8 2,144,018 - Psyr_1868 0.84 +0.4 2,144,018 - Psyr_1868 0.84 +0.6 2,144,046 + -0.1 2,144,046 + +0.4 2,144,057 + +1.2 2,144,057 + +0.6 2,144,058 - +0.6
Or see this region's nucleotide sequence