Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1846 and Psyr_1847 are separated by 747 nucleotides Psyr_1847 and Psyr_1848 are separated by 45 nucleotides Psyr_1848 and Psyr_1849 are separated by 148 nucleotides
Psyr_1846: Psyr_1846 - hypothetical protein, at 2,124,794 to 2,125,192
_1846
Psyr_1847: Psyr_1847 - Smr protein/MutS2 C-terminal, at 2,125,940 to 2,126,497
_1847
Psyr_1848: Psyr_1848 - conserved hypothetical protein, at 2,126,543 to 2,126,860
_1848
Psyr_1849: Psyr_1849 - Isochorismatase hydrolase, at 2,127,009 to 2,127,599
_1849
Position (kb)
2125
2126
2127 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2125.000 kb on - strand, within Psyr_1846 at 2125.105 kb on + strand, within Psyr_1846 at 2125.105 kb on + strand, within Psyr_1846 at 2125.106 kb on - strand, within Psyr_1846 at 2125.106 kb on - strand, within Psyr_1846 at 2125.130 kb on + strand, within Psyr_1846 at 2125.164 kb on + strand at 2125.167 kb on + strand at 2125.167 kb on + strand at 2125.167 kb on + strand at 2125.171 kb on + strand at 2125.172 kb on - strand at 2125.172 kb on - strand at 2125.268 kb on + strand at 2125.268 kb on + strand at 2125.268 kb on + strand at 2125.268 kb on + strand at 2125.268 kb on + strand at 2125.269 kb on - strand at 2125.269 kb on - strand at 2125.269 kb on - strand at 2125.269 kb on - strand at 2125.272 kb on + strand at 2125.272 kb on + strand at 2125.272 kb on + strand at 2125.272 kb on + strand at 2125.272 kb on + strand at 2125.273 kb on - strand at 2125.273 kb on - strand at 2125.310 kb on + strand at 2125.310 kb on + strand at 2125.311 kb on - strand at 2125.311 kb on - strand at 2125.311 kb on - strand at 2125.311 kb on - strand at 2125.432 kb on + strand at 2125.433 kb on - strand at 2125.490 kb on - strand at 2125.493 kb on + strand at 2125.493 kb on + strand at 2125.493 kb on + strand at 2125.493 kb on + strand at 2125.493 kb on + strand at 2125.493 kb on + strand at 2125.493 kb on + strand at 2125.493 kb on + strand at 2125.493 kb on + strand at 2125.493 kb on + strand at 2125.493 kb on + strand at 2125.494 kb on - strand at 2125.494 kb on - strand at 2125.494 kb on - strand at 2125.494 kb on - strand at 2125.494 kb on - strand at 2125.494 kb on - strand at 2125.494 kb on - strand at 2125.494 kb on - strand at 2125.494 kb on - strand at 2125.494 kb on - strand at 2125.494 kb on - strand at 2125.494 kb on - strand at 2125.494 kb on - strand at 2125.494 kb on - strand at 2125.494 kb on - strand at 2125.494 kb on - strand at 2125.494 kb on - strand at 2125.507 kb on + strand at 2125.508 kb on - strand at 2125.603 kb on - strand at 2125.614 kb on + strand at 2125.615 kb on - strand at 2125.703 kb on - strand at 2125.751 kb on + strand at 2125.751 kb on + strand at 2125.751 kb on + strand at 2125.751 kb on + strand at 2125.751 kb on + strand at 2125.751 kb on + strand at 2125.752 kb on - strand at 2125.752 kb on - strand at 2125.941 kb on + strand at 2125.983 kb on + strand at 2125.983 kb on + strand at 2125.984 kb on - strand at 2125.984 kb on - strand at 2126.135 kb on + strand, within Psyr_1847 at 2126.135 kb on + strand, within Psyr_1847 at 2126.575 kb on + strand, within Psyr_1848 at 2126.575 kb on + strand, within Psyr_1848 at 2126.575 kb on + strand, within Psyr_1848 at 2126.576 kb on - strand, within Psyr_1848 at 2126.576 kb on - strand, within Psyr_1848 at 2126.634 kb on + strand, within Psyr_1848 at 2126.634 kb on + strand, within Psyr_1848 at 2126.634 kb on + strand, within Psyr_1848 at 2126.634 kb on + strand, within Psyr_1848 at 2126.635 kb on - strand, within Psyr_1848 at 2126.635 kb on - strand, within Psyr_1848 at 2126.635 kb on - strand, within Psyr_1848 at 2126.635 kb on - strand, within Psyr_1848 at 2126.635 kb on - strand, within Psyr_1848 at 2126.635 kb on - strand, within Psyr_1848 at 2126.635 kb on - strand, within Psyr_1848 at 2126.878 kb on + strand at 2126.879 kb on - strand at 2127.164 kb on - strand, within Psyr_1849 at 2127.194 kb on + strand, within Psyr_1849
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 2,125,000 - Psyr_1846 0.52 -0.1 2,125,105 + Psyr_1846 0.78 -1.2 2,125,105 + Psyr_1846 0.78 +1.1 2,125,106 - Psyr_1846 0.78 -1.6 2,125,106 - Psyr_1846 0.78 -1.8 2,125,130 + Psyr_1846 0.84 +1.4 2,125,164 + -0.6 2,125,167 + -0.7 2,125,167 + +1.4 2,125,167 + +1.5 2,125,171 + -0.2 2,125,172 - -0.1 2,125,172 - -1.5 2,125,268 + -0.8 2,125,268 + -1.8 2,125,268 + +1.4 2,125,268 + +0.5 2,125,268 + -0.8 2,125,269 - +0.4 2,125,269 - -2.6 2,125,269 - -2.0 2,125,269 - -2.0 2,125,272 + +0.4 2,125,272 + +0.5 2,125,272 + -0.2 2,125,272 + +1.7 2,125,272 + +0.1 2,125,273 - -0.8 2,125,273 - -1.2 2,125,310 + -1.6 2,125,310 + +1.1 2,125,311 - -0.6 2,125,311 - -1.5 2,125,311 - -0.2 2,125,311 - +0.4 2,125,432 + -1.8 2,125,433 - -0.1 2,125,490 - -0.5 2,125,493 + -1.4 2,125,493 + +0.4 2,125,493 + +0.9 2,125,493 + +1.1 2,125,493 + -0.6 2,125,493 + -0.6 2,125,493 + -0.5 2,125,493 + +0.2 2,125,493 + -0.5 2,125,493 + +0.4 2,125,493 + -0.2 2,125,494 - +0.2 2,125,494 - -0.0 2,125,494 - -0.2 2,125,494 - +0.6 2,125,494 - +2.4 2,125,494 - -1.2 2,125,494 - -0.2 2,125,494 - +0.6 2,125,494 - +0.0 2,125,494 - +0.4 2,125,494 - -0.8 2,125,494 - +2.0 2,125,494 - -1.8 2,125,494 - -2.1 2,125,494 - -2.2 2,125,494 - -1.2 2,125,494 - -0.2 2,125,507 + +0.7 2,125,508 - +0.4 2,125,603 - -0.5 2,125,614 + -0.2 2,125,615 - -1.0 2,125,703 - -1.7 2,125,751 + +1.0 2,125,751 + +0.0 2,125,751 + -0.5 2,125,751 + +1.8 2,125,751 + -1.0 2,125,751 + +0.1 2,125,752 - +2.2 2,125,752 - -0.6 2,125,941 + -1.5 2,125,983 + +0.0 2,125,983 + -2.1 2,125,984 - +0.3 2,125,984 - +0.6 2,126,135 + Psyr_1847 0.35 -0.2 2,126,135 + Psyr_1847 0.35 +0.0 2,126,575 + Psyr_1848 0.10 +0.9 2,126,575 + Psyr_1848 0.10 -1.1 2,126,575 + Psyr_1848 0.10 -0.1 2,126,576 - Psyr_1848 0.10 +1.8 2,126,576 - Psyr_1848 0.10 +0.4 2,126,634 + Psyr_1848 0.29 +0.9 2,126,634 + Psyr_1848 0.29 +0.2 2,126,634 + Psyr_1848 0.29 -1.2 2,126,634 + Psyr_1848 0.29 +0.0 2,126,635 - Psyr_1848 0.29 -2.0 2,126,635 - Psyr_1848 0.29 +0.4 2,126,635 - Psyr_1848 0.29 -1.9 2,126,635 - Psyr_1848 0.29 -1.2 2,126,635 - Psyr_1848 0.29 +0.5 2,126,635 - Psyr_1848 0.29 -0.0 2,126,635 - Psyr_1848 0.29 +0.4 2,126,878 + -0.4 2,126,879 - -0.2 2,127,164 - Psyr_1849 0.26 +0.9 2,127,194 + Psyr_1849 0.31 -1.2
Or see this region's nucleotide sequence