Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1823 and Psyr_1824 are separated by 93 nucleotides Psyr_1824 and Psyr_1825 are separated by 100 nucleotides Psyr_1825 and Psyr_1826 are separated by 61 nucleotides Psyr_1826 and Psyr_1827 are separated by 137 nucleotides Psyr_1827 and Psyr_1828 are separated by 89 nucleotides
Psyr_1823: Psyr_1823 - Twin-arginine translocation pathway signal, at 2,103,171 to 2,104,856
_1823
Psyr_1824: Psyr_1824 - amino acid ABC transporter substrate-binding protein, PAAT family, at 2,104,950 to 2,105,744
_1824
Psyr_1825: Psyr_1825 - DNA polymerase III, tau subunit, at 2,105,845 to 2,108,055
_1825
Psyr_1826: Psyr_1826 - Conserved hypothetical protein 103, at 2,108,117 to 2,108,443
_1826
Psyr_1827: Psyr_1827 - SirA-like protein, at 2,108,581 to 2,108,835
_1827
Psyr_1828: Psyr_1828 - 4-phosphoerythronate dehydrogenase, at 2,108,925 to 2,110,067
_1828
Position (kb)
2105
2106
2107
2108
2109 Strain fitness (log2 ratio)
-2
-1
0
1 at 2104.865 kb on - strand at 2104.918 kb on + strand at 2104.918 kb on + strand at 2104.918 kb on + strand at 2104.919 kb on - strand at 2104.927 kb on - strand at 2104.972 kb on + strand at 2104.972 kb on + strand at 2104.972 kb on + strand at 2104.972 kb on + strand at 2104.972 kb on + strand at 2104.973 kb on - strand at 2104.973 kb on - strand at 2105.174 kb on - strand, within Psyr_1824 at 2105.174 kb on - strand, within Psyr_1824 at 2105.174 kb on - strand, within Psyr_1824 at 2105.174 kb on - strand, within Psyr_1824 at 2105.174 kb on - strand, within Psyr_1824 at 2105.282 kb on - strand, within Psyr_1824 at 2105.398 kb on + strand, within Psyr_1824 at 2105.399 kb on - strand, within Psyr_1824 at 2105.438 kb on - strand, within Psyr_1824 at 2105.438 kb on - strand, within Psyr_1824 at 2105.480 kb on + strand, within Psyr_1824 at 2105.670 kb on + strand at 2105.671 kb on - strand at 2105.724 kb on - strand at 2105.756 kb on - strand at 2105.756 kb on - strand at 2105.765 kb on - strand at 2108.111 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 2,104,865 - -2.0 2,104,918 + +0.6 2,104,918 + +1.1 2,104,918 + +1.2 2,104,919 - -0.1 2,104,927 - +0.1 2,104,972 + -0.6 2,104,972 + +0.1 2,104,972 + +1.4 2,104,972 + -2.4 2,104,972 + +0.1 2,104,973 - -0.2 2,104,973 - -1.4 2,105,174 - Psyr_1824 0.28 -0.3 2,105,174 - Psyr_1824 0.28 -0.2 2,105,174 - Psyr_1824 0.28 -0.2 2,105,174 - Psyr_1824 0.28 -0.8 2,105,174 - Psyr_1824 0.28 -1.4 2,105,282 - Psyr_1824 0.42 -1.0 2,105,398 + Psyr_1824 0.56 +0.1 2,105,399 - Psyr_1824 0.56 -1.3 2,105,438 - Psyr_1824 0.61 -1.0 2,105,438 - Psyr_1824 0.61 -1.4 2,105,480 + Psyr_1824 0.67 +0.4 2,105,670 + -0.5 2,105,671 - +0.9 2,105,724 - -0.7 2,105,756 - +0.6 2,105,756 - -1.7 2,105,765 - -0.2 2,108,111 + +0.4
Or see this region's nucleotide sequence