Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1821 and Psyr_1822 are separated by 657 nucleotides Psyr_1822 and Psyr_1823 are separated by 5 nucleotides
Psyr_1821: Psyr_1821 - GCN5-related N-acetyltransferase, at 2,100,895 to 2,101,383
_1821
Psyr_1822: Psyr_1822 - MscS Mechanosensitive ion channel, at 2,102,041 to 2,103,165
_1822
Psyr_1823: Psyr_1823 - Twin-arginine translocation pathway signal, at 2,103,171 to 2,104,856
_1823
Position (kb)
2102
2103
2104 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2101.263 kb on + strand, within Psyr_1821 at 2101.277 kb on + strand, within Psyr_1821 at 2101.278 kb on - strand, within Psyr_1821 at 2101.278 kb on - strand, within Psyr_1821 at 2101.297 kb on + strand, within Psyr_1821 at 2101.297 kb on + strand, within Psyr_1821 at 2101.298 kb on - strand, within Psyr_1821 at 2101.298 kb on - strand, within Psyr_1821 at 2101.298 kb on - strand, within Psyr_1821 at 2101.298 kb on - strand, within Psyr_1821 at 2101.312 kb on - strand, within Psyr_1821 at 2101.764 kb on - strand at 2101.773 kb on + strand at 2101.812 kb on + strand at 2101.812 kb on + strand at 2101.812 kb on + strand at 2101.812 kb on + strand at 2101.813 kb on - strand at 2101.873 kb on - strand at 2101.873 kb on - strand at 2101.873 kb on - strand at 2101.930 kb on - strand at 2102.219 kb on - strand, within Psyr_1822 at 2102.219 kb on - strand, within Psyr_1822 at 2102.219 kb on - strand, within Psyr_1822 at 2102.250 kb on + strand, within Psyr_1822 at 2102.250 kb on + strand, within Psyr_1822 at 2102.250 kb on + strand, within Psyr_1822 at 2102.251 kb on - strand, within Psyr_1822 at 2102.253 kb on + strand, within Psyr_1822 at 2102.253 kb on + strand, within Psyr_1822 at 2102.254 kb on - strand, within Psyr_1822 at 2102.254 kb on - strand, within Psyr_1822 at 2102.254 kb on - strand, within Psyr_1822 at 2102.254 kb on - strand, within Psyr_1822 at 2102.254 kb on - strand, within Psyr_1822 at 2102.254 kb on - strand, within Psyr_1822 at 2102.254 kb on - strand, within Psyr_1822 at 2102.408 kb on - strand, within Psyr_1822 at 2102.535 kb on + strand, within Psyr_1822 at 2102.535 kb on + strand, within Psyr_1822 at 2102.536 kb on - strand, within Psyr_1822 at 2102.791 kb on + strand, within Psyr_1822 at 2102.792 kb on - strand, within Psyr_1822 at 2102.839 kb on + strand, within Psyr_1822 at 2102.839 kb on + strand, within Psyr_1822 at 2102.839 kb on + strand, within Psyr_1822 at 2102.839 kb on + strand, within Psyr_1822 at 2102.839 kb on + strand, within Psyr_1822 at 2102.839 kb on + strand, within Psyr_1822 at 2102.839 kb on + strand, within Psyr_1822 at 2102.840 kb on - strand, within Psyr_1822 at 2102.840 kb on - strand, within Psyr_1822 at 2102.840 kb on - strand, within Psyr_1822 at 2102.840 kb on - strand, within Psyr_1822 at 2102.862 kb on - strand, within Psyr_1822 at 2102.995 kb on + strand, within Psyr_1822 at 2102.996 kb on - strand, within Psyr_1822 at 2102.996 kb on - strand, within Psyr_1822 at 2103.004 kb on + strand, within Psyr_1822 at 2103.004 kb on + strand, within Psyr_1822 at 2103.004 kb on + strand, within Psyr_1822 at 2103.004 kb on + strand, within Psyr_1822 at 2103.004 kb on + strand, within Psyr_1822 at 2103.004 kb on + strand, within Psyr_1822 at 2103.004 kb on + strand, within Psyr_1822 at 2103.004 kb on + strand, within Psyr_1822 at 2103.004 kb on + strand, within Psyr_1822 at 2103.005 kb on - strand, within Psyr_1822 at 2103.005 kb on - strand, within Psyr_1822 at 2103.005 kb on - strand, within Psyr_1822 at 2103.005 kb on - strand, within Psyr_1822 at 2103.005 kb on - strand, within Psyr_1822 at 2103.006 kb on + strand, within Psyr_1822 at 2103.094 kb on + strand at 2103.095 kb on - strand at 2103.117 kb on + strand at 2103.577 kb on + strand, within Psyr_1823 at 2103.577 kb on + strand, within Psyr_1823 at 2103.577 kb on + strand, within Psyr_1823 at 2103.577 kb on + strand, within Psyr_1823 at 2103.577 kb on + strand, within Psyr_1823 at 2103.578 kb on - strand, within Psyr_1823 at 2103.712 kb on + strand, within Psyr_1823 at 2103.712 kb on + strand, within Psyr_1823 at 2103.713 kb on - strand, within Psyr_1823 at 2103.734 kb on - strand, within Psyr_1823 at 2103.967 kb on + strand, within Psyr_1823 at 2103.968 kb on - strand, within Psyr_1823 at 2103.968 kb on - strand, within Psyr_1823 at 2103.968 kb on - strand, within Psyr_1823
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 2,101,263 + Psyr_1821 0.75 +0.3 2,101,277 + Psyr_1821 0.78 +0.6 2,101,278 - Psyr_1821 0.78 -1.0 2,101,278 - Psyr_1821 0.78 -0.1 2,101,297 + Psyr_1821 0.82 +0.2 2,101,297 + Psyr_1821 0.82 +0.2 2,101,298 - Psyr_1821 0.82 +0.5 2,101,298 - Psyr_1821 0.82 +0.1 2,101,298 - Psyr_1821 0.82 -1.0 2,101,298 - Psyr_1821 0.82 -0.3 2,101,312 - Psyr_1821 0.85 -0.1 2,101,764 - -1.0 2,101,773 + -1.7 2,101,812 + -0.1 2,101,812 + +0.0 2,101,812 + -1.5 2,101,812 + -0.5 2,101,813 - -0.2 2,101,873 - -1.0 2,101,873 - +0.4 2,101,873 - -3.6 2,101,930 - +0.7 2,102,219 - Psyr_1822 0.16 +1.8 2,102,219 - Psyr_1822 0.16 -0.3 2,102,219 - Psyr_1822 0.16 +0.6 2,102,250 + Psyr_1822 0.19 -0.1 2,102,250 + Psyr_1822 0.19 +0.4 2,102,250 + Psyr_1822 0.19 +0.2 2,102,251 - Psyr_1822 0.19 +0.5 2,102,253 + Psyr_1822 0.19 -0.2 2,102,253 + Psyr_1822 0.19 -0.9 2,102,254 - Psyr_1822 0.19 -0.1 2,102,254 - Psyr_1822 0.19 -0.2 2,102,254 - Psyr_1822 0.19 -0.2 2,102,254 - Psyr_1822 0.19 +1.8 2,102,254 - Psyr_1822 0.19 +0.5 2,102,254 - Psyr_1822 0.19 +0.3 2,102,254 - Psyr_1822 0.19 -0.2 2,102,408 - Psyr_1822 0.33 -1.0 2,102,535 + Psyr_1822 0.44 +0.7 2,102,535 + Psyr_1822 0.44 +0.3 2,102,536 - Psyr_1822 0.44 -0.9 2,102,791 + Psyr_1822 0.67 +1.0 2,102,792 - Psyr_1822 0.67 +0.9 2,102,839 + Psyr_1822 0.71 -0.2 2,102,839 + Psyr_1822 0.71 +0.9 2,102,839 + Psyr_1822 0.71 -0.6 2,102,839 + Psyr_1822 0.71 +1.1 2,102,839 + Psyr_1822 0.71 +0.9 2,102,839 + Psyr_1822 0.71 +2.2 2,102,839 + Psyr_1822 0.71 -1.2 2,102,840 - Psyr_1822 0.71 +0.7 2,102,840 - Psyr_1822 0.71 -0.2 2,102,840 - Psyr_1822 0.71 +0.4 2,102,840 - Psyr_1822 0.71 +1.2 2,102,862 - Psyr_1822 0.73 +0.7 2,102,995 + Psyr_1822 0.85 -0.5 2,102,996 - Psyr_1822 0.85 -0.3 2,102,996 - Psyr_1822 0.85 -0.4 2,103,004 + Psyr_1822 0.86 +0.4 2,103,004 + Psyr_1822 0.86 -0.2 2,103,004 + Psyr_1822 0.86 +0.1 2,103,004 + Psyr_1822 0.86 -0.1 2,103,004 + Psyr_1822 0.86 +1.2 2,103,004 + Psyr_1822 0.86 -0.1 2,103,004 + Psyr_1822 0.86 +0.9 2,103,004 + Psyr_1822 0.86 +0.6 2,103,004 + Psyr_1822 0.86 +0.1 2,103,005 - Psyr_1822 0.86 -0.5 2,103,005 - Psyr_1822 0.86 +1.0 2,103,005 - Psyr_1822 0.86 -0.8 2,103,005 - Psyr_1822 0.86 +0.6 2,103,005 - Psyr_1822 0.86 -2.0 2,103,006 + Psyr_1822 0.86 -1.2 2,103,094 + +0.3 2,103,095 - -0.9 2,103,117 + -0.0 2,103,577 + Psyr_1823 0.24 +0.6 2,103,577 + Psyr_1823 0.24 -0.8 2,103,577 + Psyr_1823 0.24 +0.3 2,103,577 + Psyr_1823 0.24 +1.2 2,103,577 + Psyr_1823 0.24 +0.5 2,103,578 - Psyr_1823 0.24 -0.6 2,103,712 + Psyr_1823 0.32 +0.9 2,103,712 + Psyr_1823 0.32 -0.4 2,103,713 - Psyr_1823 0.32 +1.4 2,103,734 - Psyr_1823 0.33 -0.8 2,103,967 + Psyr_1823 0.47 -0.4 2,103,968 - Psyr_1823 0.47 -0.4 2,103,968 - Psyr_1823 0.47 +2.7 2,103,968 - Psyr_1823 0.47 -1.0
Or see this region's nucleotide sequence