Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1801 and Psyr_1802 are separated by 186 nucleotides Psyr_1802 and Psyr_1803 are separated by 96 nucleotides Psyr_1803 and Psyr_1804 are separated by 176 nucleotides
Psyr_1801: Psyr_1801 - 1-aminocyclopropane-1-carboxylate deaminase, at 2,074,515 to 2,075,531
_1801
Psyr_1802: Psyr_1802 - transcriptional regulator, AsnC family, at 2,075,718 to 2,076,224
_1802
Psyr_1803: Psyr_1803 - Protein of unknown function DUF808, at 2,076,321 to 2,077,280
_1803
Psyr_1804: Psyr_1804 - conserved hypothetical protein, at 2,077,457 to 2,078,242
_1804
Position (kb)
2076
2077
2078 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2075.428 kb on + strand, within Psyr_1801 at 2075.428 kb on + strand, within Psyr_1801 at 2075.429 kb on - strand, within Psyr_1801 at 2075.429 kb on - strand, within Psyr_1801 at 2075.429 kb on - strand, within Psyr_1801 at 2075.469 kb on + strand at 2075.469 kb on + strand at 2075.470 kb on - strand at 2075.532 kb on - strand at 2075.548 kb on + strand at 2075.549 kb on - strand at 2075.571 kb on + strand at 2075.571 kb on + strand at 2075.572 kb on - strand at 2075.616 kb on + strand at 2075.761 kb on + strand at 2076.147 kb on + strand, within Psyr_1802 at 2076.147 kb on + strand, within Psyr_1802 at 2076.147 kb on + strand, within Psyr_1802 at 2076.148 kb on - strand, within Psyr_1802 at 2076.214 kb on + strand at 2076.214 kb on + strand at 2076.214 kb on + strand at 2076.214 kb on + strand at 2076.214 kb on + strand at 2076.215 kb on - strand at 2076.215 kb on - strand at 2076.215 kb on - strand at 2076.215 kb on - strand at 2076.215 kb on - strand at 2076.215 kb on - strand at 2076.215 kb on - strand at 2076.351 kb on - strand at 2076.392 kb on + strand at 2076.684 kb on - strand, within Psyr_1803 at 2076.963 kb on + strand, within Psyr_1803 at 2077.361 kb on + strand at 2077.362 kb on - strand at 2077.458 kb on + strand at 2077.458 kb on + strand at 2077.458 kb on + strand at 2077.458 kb on + strand at 2077.459 kb on - strand at 2077.459 kb on - strand at 2077.530 kb on + strand at 2077.546 kb on - strand, within Psyr_1804 at 2077.566 kb on + strand, within Psyr_1804 at 2077.566 kb on + strand, within Psyr_1804 at 2077.566 kb on + strand, within Psyr_1804 at 2077.567 kb on - strand, within Psyr_1804 at 2077.567 kb on - strand, within Psyr_1804 at 2077.567 kb on - strand, within Psyr_1804 at 2077.567 kb on - strand, within Psyr_1804 at 2077.608 kb on + strand, within Psyr_1804 at 2077.608 kb on + strand, within Psyr_1804 at 2077.608 kb on + strand, within Psyr_1804 at 2077.608 kb on + strand, within Psyr_1804 at 2077.608 kb on + strand, within Psyr_1804 at 2077.609 kb on - strand, within Psyr_1804 at 2077.609 kb on - strand, within Psyr_1804 at 2077.609 kb on - strand, within Psyr_1804 at 2077.609 kb on - strand, within Psyr_1804 at 2077.609 kb on - strand, within Psyr_1804 at 2077.609 kb on - strand, within Psyr_1804 at 2077.609 kb on - strand, within Psyr_1804 at 2077.609 kb on - strand, within Psyr_1804 at 2077.609 kb on - strand, within Psyr_1804 at 2077.644 kb on + strand, within Psyr_1804 at 2077.644 kb on + strand, within Psyr_1804 at 2077.644 kb on + strand, within Psyr_1804 at 2077.645 kb on - strand, within Psyr_1804 at 2077.731 kb on + strand, within Psyr_1804 at 2077.732 kb on - strand, within Psyr_1804 at 2077.797 kb on + strand, within Psyr_1804 at 2077.890 kb on + strand, within Psyr_1804 at 2077.890 kb on + strand, within Psyr_1804 at 2077.890 kb on + strand, within Psyr_1804 at 2077.891 kb on - strand, within Psyr_1804 at 2077.986 kb on + strand, within Psyr_1804 at 2077.986 kb on + strand, within Psyr_1804 at 2077.987 kb on - strand, within Psyr_1804 at 2077.987 kb on - strand, within Psyr_1804 at 2077.987 kb on - strand, within Psyr_1804 at 2078.026 kb on - strand, within Psyr_1804 at 2078.089 kb on + strand, within Psyr_1804 at 2078.090 kb on - strand, within Psyr_1804 at 2078.140 kb on - strand, within Psyr_1804 at 2078.149 kb on + strand, within Psyr_1804 at 2078.152 kb on + strand, within Psyr_1804 at 2078.152 kb on + strand, within Psyr_1804 at 2078.153 kb on - strand, within Psyr_1804 at 2078.153 kb on - strand, within Psyr_1804 at 2078.153 kb on - strand, within Psyr_1804 at 2078.217 kb on - strand at 2078.233 kb on + strand at 2078.261 kb on + strand at 2078.261 kb on + strand at 2078.262 kb on - strand at 2078.262 kb on - strand at 2078.262 kb on - strand at 2078.262 kb on - strand at 2078.262 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 2,075,428 + Psyr_1801 0.90 -1.3 2,075,428 + Psyr_1801 0.90 -0.7 2,075,429 - Psyr_1801 0.90 -1.0 2,075,429 - Psyr_1801 0.90 +2.1 2,075,429 - Psyr_1801 0.90 +0.4 2,075,469 + -1.1 2,075,469 + -2.2 2,075,470 - +0.4 2,075,532 - -0.7 2,075,548 + -0.0 2,075,549 - -0.3 2,075,571 + -0.1 2,075,571 + +1.0 2,075,572 - -0.0 2,075,616 + -0.2 2,075,761 + +0.8 2,076,147 + Psyr_1802 0.85 +0.0 2,076,147 + Psyr_1802 0.85 -1.4 2,076,147 + Psyr_1802 0.85 +0.5 2,076,148 - Psyr_1802 0.85 +0.4 2,076,214 + -1.8 2,076,214 + +0.3 2,076,214 + -1.1 2,076,214 + -0.8 2,076,214 + -2.1 2,076,215 - +0.5 2,076,215 - +0.8 2,076,215 - +0.3 2,076,215 - +0.1 2,076,215 - -0.5 2,076,215 - +0.1 2,076,215 - -0.2 2,076,351 - -0.4 2,076,392 + +0.8 2,076,684 - Psyr_1803 0.38 -1.0 2,076,963 + Psyr_1803 0.67 +0.8 2,077,361 + -0.9 2,077,362 - +1.3 2,077,458 + +0.7 2,077,458 + -0.1 2,077,458 + +0.6 2,077,458 + -1.0 2,077,459 - -0.8 2,077,459 - +1.0 2,077,530 + -0.5 2,077,546 - Psyr_1804 0.11 +1.7 2,077,566 + Psyr_1804 0.14 +0.8 2,077,566 + Psyr_1804 0.14 +0.0 2,077,566 + Psyr_1804 0.14 +0.0 2,077,567 - Psyr_1804 0.14 -1.1 2,077,567 - Psyr_1804 0.14 +0.7 2,077,567 - Psyr_1804 0.14 +0.7 2,077,567 - Psyr_1804 0.14 -0.1 2,077,608 + Psyr_1804 0.19 +0.7 2,077,608 + Psyr_1804 0.19 +2.6 2,077,608 + Psyr_1804 0.19 +0.3 2,077,608 + Psyr_1804 0.19 +1.0 2,077,608 + Psyr_1804 0.19 +0.4 2,077,609 - Psyr_1804 0.19 -0.4 2,077,609 - Psyr_1804 0.19 -0.3 2,077,609 - Psyr_1804 0.19 -1.2 2,077,609 - Psyr_1804 0.19 +0.1 2,077,609 - Psyr_1804 0.19 -1.4 2,077,609 - Psyr_1804 0.19 -2.5 2,077,609 - Psyr_1804 0.19 +0.0 2,077,609 - Psyr_1804 0.19 +0.8 2,077,609 - Psyr_1804 0.19 +0.1 2,077,644 + Psyr_1804 0.24 +1.4 2,077,644 + Psyr_1804 0.24 -0.1 2,077,644 + Psyr_1804 0.24 +0.9 2,077,645 - Psyr_1804 0.24 +0.9 2,077,731 + Psyr_1804 0.35 -2.0 2,077,732 - Psyr_1804 0.35 +1.0 2,077,797 + Psyr_1804 0.43 +1.2 2,077,890 + Psyr_1804 0.55 -0.2 2,077,890 + Psyr_1804 0.55 +1.4 2,077,890 + Psyr_1804 0.55 +0.7 2,077,891 - Psyr_1804 0.55 -0.5 2,077,986 + Psyr_1804 0.67 +0.8 2,077,986 + Psyr_1804 0.67 -0.3 2,077,987 - Psyr_1804 0.67 +1.4 2,077,987 - Psyr_1804 0.67 -1.8 2,077,987 - Psyr_1804 0.67 +0.7 2,078,026 - Psyr_1804 0.72 -1.3 2,078,089 + Psyr_1804 0.80 -0.4 2,078,090 - Psyr_1804 0.81 +0.4 2,078,140 - Psyr_1804 0.87 -0.7 2,078,149 + Psyr_1804 0.88 -2.1 2,078,152 + Psyr_1804 0.88 -2.1 2,078,152 + Psyr_1804 0.88 -0.7 2,078,153 - Psyr_1804 0.89 -1.0 2,078,153 - Psyr_1804 0.89 -0.6 2,078,153 - Psyr_1804 0.89 -0.6 2,078,217 - +0.6 2,078,233 + +1.0 2,078,261 + -1.3 2,078,261 + -0.9 2,078,262 - +1.2 2,078,262 - -0.4 2,078,262 - -0.2 2,078,262 - +0.1 2,078,262 - +0.4
Or see this region's nucleotide sequence