Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_1728

Experiment: KB with Aristolochic Acid 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_1726 and Psyr_1727 are separated by 387 nucleotidesPsyr_1727 and Psyr_1728 are separated by 84 nucleotidesPsyr_1728 and Psyr_1729 are separated by 149 nucleotidesPsyr_1729 and Psyr_1730 are separated by 72 nucleotides Psyr_1726: Psyr_1726 - aconitase, at 1,958,920 to 1,961,535 _1726 Psyr_1727: Psyr_1727 - Protein of unknown function DUF1289, at 1,961,923 to 1,962,393 _1727 Psyr_1728: Psyr_1728 - tRNA--hydroxylase, at 1,962,478 to 1,963,089 _1728 Psyr_1729: Psyr_1729 - transcriptional regulator, MarR family, at 1,963,239 to 1,963,727 _1729 Psyr_1730: Psyr_1730 - Secretion protein HlyD, at 1,963,800 to 1,965,008 _1730 Position (kb) 1962 1963 1964Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1961.840 kb on - strandat 1961.847 kb on - strandat 1962.352 kb on + strandat 1962.352 kb on + strandat 1962.353 kb on - strandat 1962.353 kb on - strandat 1962.353 kb on - strandat 1962.438 kb on + strandat 1962.438 kb on + strandat 1962.438 kb on + strandat 1962.438 kb on + strandat 1962.438 kb on + strandat 1962.438 kb on + strandat 1962.438 kb on + strandat 1962.439 kb on - strandat 1962.439 kb on - strandat 1962.439 kb on - strandat 1962.439 kb on - strandat 1962.441 kb on - strandat 1962.477 kb on + strandat 1962.477 kb on + strandat 1962.477 kb on + strandat 1962.477 kb on + strandat 1962.477 kb on + strandat 1962.477 kb on + strandat 1962.477 kb on + strandat 1962.477 kb on + strandat 1962.478 kb on - strandat 1962.478 kb on - strandat 1962.478 kb on - strandat 1962.478 kb on - strandat 1962.478 kb on - strandat 1962.478 kb on - strandat 1962.478 kb on - strandat 1962.478 kb on - strandat 1962.478 kb on - strandat 1962.478 kb on - strandat 1962.478 kb on - strandat 1962.631 kb on + strand, within Psyr_1728at 1962.632 kb on - strand, within Psyr_1728at 1962.632 kb on - strand, within Psyr_1728at 1962.761 kb on - strand, within Psyr_1728at 1962.907 kb on + strand, within Psyr_1728at 1962.950 kb on - strand, within Psyr_1728at 1963.073 kb on + strandat 1963.074 kb on - strandat 1963.151 kb on - strandat 1963.153 kb on + strandat 1963.166 kb on - strandat 1963.188 kb on + strandat 1963.198 kb on + strandat 1963.199 kb on - strandat 1963.224 kb on + strandat 1963.224 kb on + strandat 1963.225 kb on - strandat 1963.225 kb on - strandat 1963.225 kb on - strandat 1963.299 kb on - strand, within Psyr_1729at 1963.332 kb on + strand, within Psyr_1729at 1963.333 kb on - strand, within Psyr_1729at 1963.333 kb on - strand, within Psyr_1729at 1963.333 kb on - strand, within Psyr_1729at 1963.344 kb on + strand, within Psyr_1729at 1963.344 kb on + strand, within Psyr_1729at 1963.345 kb on - strand, within Psyr_1729at 1963.351 kb on + strand, within Psyr_1729at 1963.352 kb on - strand, within Psyr_1729at 1963.352 kb on - strand, within Psyr_1729at 1963.719 kb on - strandat 1963.786 kb on + strandat 1963.792 kb on + strandat 1963.932 kb on + strand, within Psyr_1730at 1963.941 kb on + strand, within Psyr_1730at 1963.941 kb on + strand, within Psyr_1730at 1963.942 kb on - strand, within Psyr_1730at 1963.974 kb on + strandat 1963.974 kb on + strand, within Psyr_1730at 1963.974 kb on + strand, within Psyr_1730at 1963.974 kb on + strand, within Psyr_1730at 1963.974 kb on + strand, within Psyr_1730at 1963.974 kb on + strand, within Psyr_1730at 1963.975 kb on - strand, within Psyr_1730at 1963.975 kb on - strand, within Psyr_1730at 1963.975 kb on - strand, within Psyr_1730at 1963.975 kb on - strand, within Psyr_1730at 1963.975 kb on - strand, within Psyr_1730at 1964.030 kb on + strand, within Psyr_1730at 1964.031 kb on - strand, within Psyr_1730

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM
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1,961,840 - +0.4
1,961,847 - -0.6
1,962,352 + -0.5
1,962,352 + +0.1
1,962,353 - -0.3
1,962,353 - -0.6
1,962,353 - +1.2
1,962,438 + -0.2
1,962,438 + +0.6
1,962,438 + +0.6
1,962,438 + +0.5
1,962,438 + -1.4
1,962,438 + +0.9
1,962,438 + +0.2
1,962,439 - -1.0
1,962,439 - -0.6
1,962,439 - -0.1
1,962,439 - +0.8
1,962,441 - -1.0
1,962,477 + +1.0
1,962,477 + -0.3
1,962,477 + -0.5
1,962,477 + +0.1
1,962,477 + +0.3
1,962,477 + -1.2
1,962,477 + -1.8
1,962,477 + +0.1
1,962,478 - +0.8
1,962,478 - -0.5
1,962,478 - +0.1
1,962,478 - +0.7
1,962,478 - -0.1
1,962,478 - +0.8
1,962,478 - -1.0
1,962,478 - +0.2
1,962,478 - +0.1
1,962,478 - -1.0
1,962,478 - +0.9
1,962,631 + Psyr_1728 0.25 -1.8
1,962,632 - Psyr_1728 0.25 -3.1
1,962,632 - Psyr_1728 0.25 -0.3
1,962,761 - Psyr_1728 0.46 -0.8
1,962,907 + Psyr_1728 0.70 -0.0
1,962,950 - Psyr_1728 0.77 -0.7
1,963,073 + +0.1
1,963,074 - -0.4
1,963,151 - -0.4
1,963,153 + -0.2
1,963,166 - -2.6
1,963,188 + +0.5
1,963,198 + -1.3
1,963,199 - +0.2
1,963,224 + -0.3
1,963,224 + +0.1
1,963,225 - -0.2
1,963,225 - -1.3
1,963,225 - +0.3
1,963,299 - Psyr_1729 0.12 -1.1
1,963,332 + Psyr_1729 0.19 +1.7
1,963,333 - Psyr_1729 0.19 -0.4
1,963,333 - Psyr_1729 0.19 -2.1
1,963,333 - Psyr_1729 0.19 +0.9
1,963,344 + Psyr_1729 0.21 -0.7
1,963,344 + Psyr_1729 0.21 -0.0
1,963,345 - Psyr_1729 0.22 +0.3
1,963,351 + Psyr_1729 0.23 -0.9
1,963,352 - Psyr_1729 0.23 -0.5
1,963,352 - Psyr_1729 0.23 +0.7
1,963,719 - +0.2
1,963,786 + +2.1
1,963,792 + -0.7
1,963,932 + Psyr_1730 0.11 +1.8
1,963,941 + Psyr_1730 0.12 -0.1
1,963,941 + Psyr_1730 0.12 +0.5
1,963,942 - Psyr_1730 0.12 +0.5
1,963,974 + -1.4
1,963,974 + Psyr_1730 0.14 +0.4
1,963,974 + Psyr_1730 0.14 +0.1
1,963,974 + Psyr_1730 0.14 +0.1
1,963,974 + Psyr_1730 0.14 +0.8
1,963,974 + Psyr_1730 0.14 +0.6
1,963,975 - Psyr_1730 0.14 -0.6
1,963,975 - Psyr_1730 0.14 +0.0
1,963,975 - Psyr_1730 0.14 +0.3
1,963,975 - Psyr_1730 0.14 -0.4
1,963,975 - Psyr_1730 0.14 -0.6
1,964,030 + Psyr_1730 0.19 +1.4
1,964,031 - Psyr_1730 0.19 +1.0

Or see this region's nucleotide sequence