Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1691 and Psyr_1692 are separated by 12 nucleotides Psyr_1692 and Psyr_1693 are separated by 26 nucleotides Psyr_1693 and Psyr_1694 are separated by 30 nucleotides Psyr_1694 and Psyr_1695 are separated by 84 nucleotides
Psyr_1691: Psyr_1691 - hypothetical protein, at 1,900,590 to 1,900,832
_1691
Psyr_1692: Psyr_1692 - conserved hypothetical protein, at 1,900,845 to 1,901,498
_1692
Psyr_1693: Psyr_1693 - PspA/IM30, at 1,901,525 to 1,902,232
_1693
Psyr_1694: Psyr_1694 - Rhomboid-like protein, at 1,902,263 to 1,902,817
_1694
Psyr_1695: Psyr_1695 - conserved hypothetical protein, at 1,902,902 to 1,903,567
_1695
Position (kb)
1901
1902
1903 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1900.584 kb on + strand at 1900.585 kb on - strand at 1900.628 kb on + strand, within Psyr_1691 at 1900.712 kb on + strand, within Psyr_1691 at 1900.712 kb on + strand, within Psyr_1691 at 1900.712 kb on + strand, within Psyr_1691 at 1900.712 kb on + strand, within Psyr_1691 at 1900.712 kb on + strand, within Psyr_1691 at 1900.713 kb on - strand, within Psyr_1691 at 1900.734 kb on + strand, within Psyr_1691 at 1900.734 kb on + strand, within Psyr_1691 at 1900.777 kb on + strand, within Psyr_1691 at 1900.777 kb on + strand, within Psyr_1691 at 1900.781 kb on + strand, within Psyr_1691 at 1900.800 kb on + strand, within Psyr_1691 at 1900.800 kb on + strand, within Psyr_1691 at 1900.800 kb on + strand, within Psyr_1691 at 1900.801 kb on - strand, within Psyr_1691 at 1900.801 kb on - strand, within Psyr_1691 at 1900.801 kb on - strand, within Psyr_1691 at 1900.809 kb on + strand at 1900.809 kb on + strand at 1900.810 kb on - strand at 1900.854 kb on - strand at 1900.854 kb on - strand at 1900.911 kb on + strand, within Psyr_1692 at 1900.911 kb on + strand, within Psyr_1692 at 1900.911 kb on + strand, within Psyr_1692 at 1900.911 kb on + strand, within Psyr_1692 at 1900.912 kb on - strand, within Psyr_1692 at 1900.912 kb on - strand, within Psyr_1692 at 1900.912 kb on - strand, within Psyr_1692 at 1900.912 kb on - strand, within Psyr_1692 at 1901.045 kb on - strand, within Psyr_1692 at 1901.045 kb on - strand, within Psyr_1692 at 1901.073 kb on - strand, within Psyr_1692 at 1901.196 kb on + strand, within Psyr_1692 at 1901.214 kb on + strand, within Psyr_1692 at 1901.214 kb on + strand, within Psyr_1692 at 1901.215 kb on - strand, within Psyr_1692 at 1901.215 kb on - strand, within Psyr_1692 at 1901.224 kb on + strand, within Psyr_1692 at 1901.225 kb on - strand, within Psyr_1692 at 1901.494 kb on - strand at 1901.494 kb on - strand at 1901.530 kb on + strand at 1901.530 kb on + strand at 1901.530 kb on + strand at 1901.530 kb on + strand at 1901.530 kb on + strand at 1901.530 kb on + strand at 1901.530 kb on - strand at 1901.531 kb on - strand at 1901.531 kb on - strand at 1901.531 kb on - strand at 1901.536 kb on + strand at 1901.536 kb on + strand at 1901.536 kb on + strand at 1901.537 kb on - strand at 1901.799 kb on + strand, within Psyr_1693 at 1901.995 kb on + strand, within Psyr_1693 at 1902.190 kb on - strand at 1902.190 kb on - strand at 1902.190 kb on - strand at 1902.574 kb on + strand, within Psyr_1694 at 1902.575 kb on - strand, within Psyr_1694 at 1902.575 kb on - strand, within Psyr_1694 at 1902.575 kb on - strand, within Psyr_1694 at 1902.617 kb on + strand, within Psyr_1694 at 1902.638 kb on + strand, within Psyr_1694 at 1902.866 kb on + strand at 1902.866 kb on + strand at 1902.866 kb on + strand at 1902.866 kb on + strand at 1902.866 kb on + strand at 1902.866 kb on + strand at 1902.867 kb on - strand at 1902.867 kb on - strand at 1902.867 kb on - strand at 1902.868 kb on + strand at 1902.868 kb on + strand at 1902.868 kb on + strand at 1902.868 kb on + strand at 1902.868 kb on + strand at 1902.868 kb on + strand at 1902.868 kb on + strand at 1902.868 kb on + strand at 1902.868 kb on + strand at 1902.868 kb on + strand at 1902.868 kb on + strand at 1902.868 kb on + strand at 1902.868 kb on + strand at 1902.868 kb on + strand at 1902.869 kb on - strand at 1902.869 kb on - strand at 1902.869 kb on - strand at 1902.869 kb on - strand at 1902.869 kb on - strand at 1902.869 kb on - strand at 1902.869 kb on - strand at 1902.869 kb on - strand at 1902.869 kb on - strand at 1902.869 kb on - strand at 1902.869 kb on - strand at 1902.869 kb on - strand at 1902.869 kb on - strand at 1902.869 kb on - strand at 1902.869 kb on - strand at 1902.869 kb on - strand at 1902.869 kb on - strand at 1902.869 kb on - strand at 1902.869 kb on - strand at 1902.871 kb on - strand at 1902.871 kb on - strand at 1902.871 kb on - strand at 1902.871 kb on - strand at 1903.140 kb on - strand, within Psyr_1695 at 1903.157 kb on + strand, within Psyr_1695 at 1903.157 kb on + strand, within Psyr_1695 at 1903.157 kb on + strand, within Psyr_1695 at 1903.158 kb on - strand, within Psyr_1695 at 1903.201 kb on - strand, within Psyr_1695 at 1903.224 kb on - strand, within Psyr_1695
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 1,900,584 + -1.8 1,900,585 - +1.0 1,900,628 + Psyr_1691 0.16 +1.7 1,900,712 + Psyr_1691 0.50 +0.8 1,900,712 + Psyr_1691 0.50 -1.7 1,900,712 + Psyr_1691 0.50 -0.1 1,900,712 + Psyr_1691 0.50 +1.2 1,900,712 + Psyr_1691 0.50 -1.6 1,900,713 - Psyr_1691 0.51 -0.6 1,900,734 + Psyr_1691 0.59 +1.0 1,900,734 + Psyr_1691 0.59 +1.0 1,900,777 + Psyr_1691 0.77 -0.4 1,900,777 + Psyr_1691 0.77 -0.0 1,900,781 + Psyr_1691 0.79 +0.2 1,900,800 + Psyr_1691 0.86 +0.1 1,900,800 + Psyr_1691 0.86 +1.0 1,900,800 + Psyr_1691 0.86 -0.5 1,900,801 - Psyr_1691 0.87 -1.0 1,900,801 - Psyr_1691 0.87 +0.8 1,900,801 - Psyr_1691 0.87 -0.6 1,900,809 + -1.0 1,900,809 + +0.8 1,900,810 - +0.3 1,900,854 - +0.5 1,900,854 - +1.4 1,900,911 + Psyr_1692 0.10 -2.1 1,900,911 + Psyr_1692 0.10 -0.7 1,900,911 + Psyr_1692 0.10 +0.4 1,900,911 + Psyr_1692 0.10 -1.5 1,900,912 - Psyr_1692 0.10 +0.1 1,900,912 - Psyr_1692 0.10 +0.6 1,900,912 - Psyr_1692 0.10 -0.5 1,900,912 - Psyr_1692 0.10 +1.1 1,901,045 - Psyr_1692 0.31 -0.3 1,901,045 - Psyr_1692 0.31 -0.4 1,901,073 - Psyr_1692 0.35 -0.0 1,901,196 + Psyr_1692 0.54 +0.0 1,901,214 + Psyr_1692 0.56 -1.8 1,901,214 + Psyr_1692 0.56 -1.4 1,901,215 - Psyr_1692 0.57 +0.7 1,901,215 - Psyr_1692 0.57 -1.8 1,901,224 + Psyr_1692 0.58 -0.5 1,901,225 - Psyr_1692 0.58 -2.0 1,901,494 - +1.4 1,901,494 - +2.1 1,901,530 + -1.4 1,901,530 + -0.3 1,901,530 + +0.9 1,901,530 + -0.4 1,901,530 + -0.4 1,901,530 + -1.6 1,901,530 - -0.6 1,901,531 - -1.0 1,901,531 - -0.4 1,901,531 - -1.2 1,901,536 + -0.8 1,901,536 + -2.5 1,901,536 + +0.3 1,901,537 - +0.4 1,901,799 + Psyr_1693 0.39 -0.5 1,901,995 + Psyr_1693 0.66 +1.3 1,902,190 - -1.0 1,902,190 - -0.4 1,902,190 - +0.4 1,902,574 + Psyr_1694 0.56 -0.2 1,902,575 - Psyr_1694 0.56 +1.1 1,902,575 - Psyr_1694 0.56 -1.2 1,902,575 - Psyr_1694 0.56 +0.0 1,902,617 + Psyr_1694 0.64 -0.8 1,902,638 + Psyr_1694 0.68 +0.4 1,902,866 + +0.4 1,902,866 + +0.3 1,902,866 + -0.6 1,902,866 + +1.8 1,902,866 + +1.1 1,902,866 + +0.3 1,902,867 - -1.8 1,902,867 - -0.2 1,902,867 - -0.6 1,902,868 + -0.8 1,902,868 + +0.7 1,902,868 + +2.0 1,902,868 + -2.6 1,902,868 + +0.1 1,902,868 + -0.8 1,902,868 + -0.2 1,902,868 + +0.0 1,902,868 + -0.8 1,902,868 + +0.3 1,902,868 + -0.3 1,902,868 + -0.0 1,902,868 + -0.0 1,902,868 + +0.8 1,902,869 - +0.4 1,902,869 - -1.0 1,902,869 - -1.0 1,902,869 - -1.2 1,902,869 - -1.2 1,902,869 - -0.6 1,902,869 - -2.0 1,902,869 - +0.0 1,902,869 - +0.4 1,902,869 - +0.4 1,902,869 - -2.0 1,902,869 - -1.8 1,902,869 - -0.2 1,902,869 - +0.4 1,902,869 - +1.4 1,902,869 - +0.4 1,902,869 - +1.0 1,902,869 - -1.4 1,902,869 - +0.2 1,902,871 - -0.6 1,902,871 - -0.4 1,902,871 - +0.6 1,902,871 - -2.0 1,903,140 - Psyr_1695 0.36 +1.1 1,903,157 + Psyr_1695 0.38 +0.6 1,903,157 + Psyr_1695 0.38 -2.0 1,903,157 + Psyr_1695 0.38 +0.4 1,903,158 - Psyr_1695 0.38 -0.3 1,903,201 - Psyr_1695 0.45 -2.9 1,903,224 - Psyr_1695 0.48 +0.5
Or see this region's nucleotide sequence