Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1610 and Psyr_1611 are separated by 90 nucleotides Psyr_1611 and Psyr_1612 overlap by 1 nucleotides Psyr_1612 and Psyr_1613 are separated by 231 nucleotides
Psyr_1610: Psyr_1610 - ribosomal large subunit pseudouridine synthase A, at 1,813,864 to 1,814,499
_1610
Psyr_1611: Psyr_1611 - cell division topological specificity factor MinE, at 1,814,590 to 1,814,844
_1611
Psyr_1612: Psyr_1612 - septum site-determining protein MinD, at 1,814,844 to 1,815,656
_1612
Psyr_1613: Psyr_1613 - septum site-determining protein MinC, at 1,815,888 to 1,816,628
_1613
Position (kb) 1814
1815
1816 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1813.845 kb on - strand at 1814.078 kb on + strand, within Psyr_1610 at 1814.078 kb on + strand, within Psyr_1610 at 1814.078 kb on + strand, within Psyr_1610 at 1814.078 kb on + strand, within Psyr_1610 at 1814.079 kb on - strand, within Psyr_1610 at 1814.079 kb on - strand, within Psyr_1610 at 1814.079 kb on - strand, within Psyr_1610 at 1814.079 kb on - strand, within Psyr_1610 at 1814.235 kb on - strand, within Psyr_1610 at 1814.235 kb on - strand, within Psyr_1610 at 1814.235 kb on - strand, within Psyr_1610 at 1814.346 kb on + strand, within Psyr_1610 at 1814.883 kb on - strand at 1815.313 kb on + strand, within Psyr_1612 at 1815.314 kb on - strand, within Psyr_1612 at 1815.636 kb on - strand at 1815.770 kb on - strand at 1815.791 kb on - strand at 1815.791 kb on - strand at 1815.791 kb on - strand at 1815.791 kb on - strand at 1815.791 kb on - strand at 1815.982 kb on + strand, within Psyr_1613 at 1815.982 kb on + strand, within Psyr_1613 at 1815.982 kb on + strand, within Psyr_1613 at 1815.983 kb on - strand, within Psyr_1613 at 1815.983 kb on - strand, within Psyr_1613 at 1816.088 kb on - strand, within Psyr_1613 at 1816.168 kb on + strand, within Psyr_1613 at 1816.168 kb on + strand, within Psyr_1613 at 1816.168 kb on + strand, within Psyr_1613 at 1816.168 kb on + strand, within Psyr_1613 at 1816.169 kb on - strand, within Psyr_1613 at 1816.169 kb on - strand, within Psyr_1613 at 1816.169 kb on - strand, within Psyr_1613 at 1816.473 kb on - strand, within Psyr_1613 at 1816.594 kb on - strand at 1816.594 kb on - strand at 1816.635 kb on + strand at 1816.635 kb on + strand at 1816.636 kb on - strand at 1816.637 kb on + strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 1,813,845 - +0.5 1,814,078 + Psyr_1610 0.34 -0.5 1,814,078 + Psyr_1610 0.34 +0.1 1,814,078 + Psyr_1610 0.34 -1.2 1,814,078 + Psyr_1610 0.34 +0.1 1,814,079 - Psyr_1610 0.34 -1.1 1,814,079 - Psyr_1610 0.34 -0.6 1,814,079 - Psyr_1610 0.34 -1.8 1,814,079 - Psyr_1610 0.34 -0.3 1,814,235 - Psyr_1610 0.58 +0.3 1,814,235 - Psyr_1610 0.58 +1.0 1,814,235 - Psyr_1610 0.58 -0.1 1,814,346 + Psyr_1610 0.76 -0.2 1,814,883 - +1.4 1,815,313 + Psyr_1612 0.58 +0.3 1,815,314 - Psyr_1612 0.58 -0.9 1,815,636 - +1.4 1,815,770 - -0.1 1,815,791 - +0.7 1,815,791 - +0.2 1,815,791 - -0.7 1,815,791 - -1.0 1,815,791 - -0.0 1,815,982 + Psyr_1613 0.13 -2.0 1,815,982 + Psyr_1613 0.13 -0.9 1,815,982 + Psyr_1613 0.13 -0.2 1,815,983 - Psyr_1613 0.13 -1.4 1,815,983 - Psyr_1613 0.13 -1.6 1,816,088 - Psyr_1613 0.27 -0.2 1,816,168 + Psyr_1613 0.38 -0.9 1,816,168 + Psyr_1613 0.38 -0.9 1,816,168 + Psyr_1613 0.38 -0.2 1,816,168 + Psyr_1613 0.38 -0.6 1,816,169 - Psyr_1613 0.38 -1.4 1,816,169 - Psyr_1613 0.38 -2.0 1,816,169 - Psyr_1613 0.38 -0.6 1,816,473 - Psyr_1613 0.79 -0.9 1,816,594 - -1.2 1,816,594 - +3.0 1,816,635 + -0.6 1,816,635 + -0.2 1,816,636 - -1.7 1,816,637 + +1.4 1,816,638 - -0.6 1,816,638 - +1.4 1,816,638 - -1.2 1,816,638 - +0.1 1,816,638 - -0.6 1,816,638 - +0.7 1,816,638 - -0.7 1,816,638 - -0.6 1,816,638 - +1.8
Or see this region's nucleotide sequence