Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1473 and Psyr_1474 are separated by 287 nucleotides Psyr_1474 and Psyr_1475 are separated by 282 nucleotides
Psyr_1473: Psyr_1473 - DNA topoisomerase III, at 1,659,653 to 1,661,674
_1473
Psyr_1474: Psyr_1474 - hypothetical protein, at 1,661,962 to 1,663,512
_1474
Psyr_1475: Psyr_1475 - Phage integrase:Phage integrase, N-terminal SAM-like protein, at 1,663,795 to 1,664,757
_1475
Position (kb)
1661
1662
1663
1664 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1660.962 kb on + strand, within Psyr_1473 at 1660.962 kb on + strand, within Psyr_1473 at 1660.962 kb on + strand, within Psyr_1473 at 1661.057 kb on - strand, within Psyr_1473 at 1661.174 kb on - strand, within Psyr_1473 at 1661.328 kb on + strand, within Psyr_1473 at 1661.328 kb on + strand, within Psyr_1473 at 1661.328 kb on + strand, within Psyr_1473 at 1661.664 kb on + strand at 1661.664 kb on + strand at 1661.664 kb on + strand at 1661.665 kb on - strand at 1661.701 kb on + strand at 1661.721 kb on + strand at 1661.721 kb on + strand at 1661.721 kb on + strand at 1661.817 kb on + strand at 1661.827 kb on + strand at 1661.827 kb on + strand at 1661.828 kb on - strand at 1661.828 kb on - strand at 1661.831 kb on + strand at 1661.831 kb on + strand at 1661.831 kb on + strand at 1661.831 kb on + strand at 1661.831 kb on + strand at 1661.831 kb on + strand at 1661.832 kb on - strand at 1661.832 kb on - strand at 1661.837 kb on + strand at 1661.838 kb on - strand at 1661.838 kb on - strand at 1661.923 kb on + strand at 1661.924 kb on - strand at 1661.924 kb on - strand at 1661.963 kb on + strand at 1661.964 kb on - strand at 1661.990 kb on + strand at 1661.991 kb on - strand at 1661.991 kb on - strand at 1662.044 kb on + strand at 1662.089 kb on - strand at 1662.103 kb on + strand at 1662.104 kb on - strand at 1662.109 kb on + strand at 1662.110 kb on - strand at 1662.138 kb on + strand, within Psyr_1474 at 1662.138 kb on + strand, within Psyr_1474 at 1662.138 kb on + strand, within Psyr_1474 at 1662.139 kb on - strand, within Psyr_1474 at 1662.139 kb on - strand, within Psyr_1474 at 1662.205 kb on + strand, within Psyr_1474 at 1662.206 kb on - strand, within Psyr_1474 at 1662.206 kb on - strand, within Psyr_1474 at 1662.253 kb on + strand, within Psyr_1474 at 1662.257 kb on + strand, within Psyr_1474 at 1662.258 kb on - strand, within Psyr_1474 at 1662.258 kb on - strand, within Psyr_1474 at 1662.316 kb on + strand, within Psyr_1474 at 1662.320 kb on + strand, within Psyr_1474 at 1662.341 kb on - strand, within Psyr_1474 at 1662.446 kb on + strand, within Psyr_1474 at 1662.447 kb on - strand at 1662.447 kb on - strand, within Psyr_1474 at 1662.504 kb on + strand, within Psyr_1474 at 1662.547 kb on - strand at 1662.633 kb on + strand, within Psyr_1474 at 1662.639 kb on - strand, within Psyr_1474 at 1662.639 kb on - strand, within Psyr_1474 at 1662.639 kb on - strand, within Psyr_1474 at 1662.640 kb on + strand, within Psyr_1474 at 1662.653 kb on - strand, within Psyr_1474 at 1662.670 kb on + strand, within Psyr_1474 at 1662.670 kb on + strand, within Psyr_1474 at 1662.671 kb on - strand, within Psyr_1474 at 1662.671 kb on - strand, within Psyr_1474 at 1662.698 kb on - strand, within Psyr_1474 at 1662.740 kb on + strand, within Psyr_1474 at 1662.745 kb on + strand, within Psyr_1474 at 1662.901 kb on + strand, within Psyr_1474 at 1662.921 kb on + strand, within Psyr_1474 at 1662.937 kb on + strand, within Psyr_1474 at 1662.938 kb on - strand, within Psyr_1474 at 1662.938 kb on - strand, within Psyr_1474 at 1662.957 kb on + strand, within Psyr_1474 at 1662.957 kb on + strand, within Psyr_1474 at 1663.004 kb on - strand, within Psyr_1474 at 1663.007 kb on - strand, within Psyr_1474 at 1663.047 kb on - strand, within Psyr_1474 at 1663.093 kb on + strand at 1663.181 kb on + strand, within Psyr_1474 at 1663.182 kb on - strand, within Psyr_1474 at 1663.276 kb on + strand, within Psyr_1474 at 1663.315 kb on + strand, within Psyr_1474 at 1663.330 kb on - strand, within Psyr_1474 at 1663.403 kb on + strand at 1663.456 kb on + strand at 1663.457 kb on - strand at 1663.457 kb on - strand at 1663.500 kb on + strand at 1663.501 kb on - strand at 1663.514 kb on + strand at 1663.535 kb on + strand at 1663.555 kb on + strand at 1663.555 kb on + strand at 1663.556 kb on - strand at 1663.566 kb on + strand at 1663.566 kb on + strand at 1663.654 kb on - strand at 1663.665 kb on + strand at 1663.666 kb on - strand at 1663.666 kb on - strand at 1663.666 kb on - strand at 1663.678 kb on + strand at 1663.678 kb on + strand at 1663.679 kb on - strand at 1663.679 kb on - strand at 1663.713 kb on - strand at 1663.732 kb on - strand at 1663.953 kb on + strand, within Psyr_1475 at 1663.954 kb on - strand, within Psyr_1475 at 1663.954 kb on - strand, within Psyr_1475 at 1664.148 kb on - strand, within Psyr_1475 at 1664.244 kb on + strand, within Psyr_1475 at 1664.244 kb on + strand, within Psyr_1475 at 1664.244 kb on + strand, within Psyr_1475 at 1664.244 kb on + strand, within Psyr_1475 at 1664.245 kb on - strand, within Psyr_1475 at 1664.245 kb on - strand, within Psyr_1475 at 1664.245 kb on - strand, within Psyr_1475 at 1664.245 kb on - strand, within Psyr_1475 at 1664.245 kb on - strand, within Psyr_1475 at 1664.245 kb on - strand, within Psyr_1475 at 1664.245 kb on - strand, within Psyr_1475 at 1664.350 kb on - strand, within Psyr_1475 at 1664.392 kb on + strand, within Psyr_1475 at 1664.424 kb on + strand, within Psyr_1475 at 1664.425 kb on - strand, within Psyr_1475 at 1664.435 kb on - strand, within Psyr_1475 at 1664.499 kb on + strand, within Psyr_1475 at 1664.499 kb on + strand, within Psyr_1475 at 1664.499 kb on + strand, within Psyr_1475 at 1664.500 kb on - strand, within Psyr_1475 at 1664.500 kb on - strand, within Psyr_1475 at 1664.500 kb on - strand, within Psyr_1475
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 1,660,962 + Psyr_1473 0.65 -0.2 1,660,962 + Psyr_1473 0.65 +1.9 1,660,962 + Psyr_1473 0.65 +0.5 1,661,057 - Psyr_1473 0.69 +0.1 1,661,174 - Psyr_1473 0.75 -0.4 1,661,328 + Psyr_1473 0.83 +0.9 1,661,328 + Psyr_1473 0.83 -0.1 1,661,328 + Psyr_1473 0.83 +1.5 1,661,664 + -0.1 1,661,664 + -0.6 1,661,664 + -0.7 1,661,665 - +1.9 1,661,701 + -0.5 1,661,721 + +1.0 1,661,721 + -0.2 1,661,721 + +0.8 1,661,817 + -1.1 1,661,827 + +0.2 1,661,827 + -0.2 1,661,828 - +0.3 1,661,828 - +0.3 1,661,831 + -0.6 1,661,831 + +0.5 1,661,831 + -0.4 1,661,831 + -0.0 1,661,831 + +0.7 1,661,831 + +0.5 1,661,832 - -0.3 1,661,832 - +0.9 1,661,837 + -2.4 1,661,838 - +0.2 1,661,838 - -0.6 1,661,923 + +1.5 1,661,924 - +0.2 1,661,924 - -0.7 1,661,963 + +0.5 1,661,964 - +0.0 1,661,990 + +0.5 1,661,991 - -1.0 1,661,991 - -0.5 1,662,044 + +1.5 1,662,089 - -1.4 1,662,103 + +2.5 1,662,104 - +0.7 1,662,109 + -1.1 1,662,110 - -0.5 1,662,138 + Psyr_1474 0.11 -0.9 1,662,138 + Psyr_1474 0.11 -1.9 1,662,138 + Psyr_1474 0.11 -0.1 1,662,139 - Psyr_1474 0.11 +1.5 1,662,139 - Psyr_1474 0.11 -2.8 1,662,205 + Psyr_1474 0.16 +0.5 1,662,206 - Psyr_1474 0.16 -1.7 1,662,206 - Psyr_1474 0.16 -1.2 1,662,253 + Psyr_1474 0.19 +0.3 1,662,257 + Psyr_1474 0.19 -1.4 1,662,258 - Psyr_1474 0.19 +0.5 1,662,258 - Psyr_1474 0.19 +0.5 1,662,316 + Psyr_1474 0.23 +0.5 1,662,320 + Psyr_1474 0.23 +0.6 1,662,341 - Psyr_1474 0.24 -2.4 1,662,446 + Psyr_1474 0.31 -1.0 1,662,447 - +1.5 1,662,447 - Psyr_1474 0.31 +1.5 1,662,504 + Psyr_1474 0.35 +1.0 1,662,547 - +1.5 1,662,633 + Psyr_1474 0.43 -0.9 1,662,639 - Psyr_1474 0.44 +0.6 1,662,639 - Psyr_1474 0.44 -1.2 1,662,639 - Psyr_1474 0.44 +0.1 1,662,640 + Psyr_1474 0.44 +0.3 1,662,653 - Psyr_1474 0.45 +0.5 1,662,670 + Psyr_1474 0.46 -1.0 1,662,670 + Psyr_1474 0.46 -1.5 1,662,671 - Psyr_1474 0.46 -0.1 1,662,671 - Psyr_1474 0.46 -0.1 1,662,698 - Psyr_1474 0.47 -3.6 1,662,740 + Psyr_1474 0.50 -1.1 1,662,745 + Psyr_1474 0.50 -1.3 1,662,901 + Psyr_1474 0.61 +0.2 1,662,921 + Psyr_1474 0.62 -1.4 1,662,937 + Psyr_1474 0.63 +1.5 1,662,938 - Psyr_1474 0.63 -0.1 1,662,938 - Psyr_1474 0.63 +1.5 1,662,957 + Psyr_1474 0.64 +0.5 1,662,957 + Psyr_1474 0.64 +0.7 1,663,004 - Psyr_1474 0.67 +0.5 1,663,007 - Psyr_1474 0.67 -0.3 1,663,047 - Psyr_1474 0.70 -0.2 1,663,093 + +1.5 1,663,181 + Psyr_1474 0.79 -0.7 1,663,182 - Psyr_1474 0.79 -3.1 1,663,276 + Psyr_1474 0.85 +0.7 1,663,315 + Psyr_1474 0.87 -1.7 1,663,330 - Psyr_1474 0.88 +0.7 1,663,403 + -0.8 1,663,456 + -0.5 1,663,457 - +1.5 1,663,457 - -0.5 1,663,500 + -0.2 1,663,501 - +1.5 1,663,514 + +1.3 1,663,535 + +1.9 1,663,555 + -0.4 1,663,555 + -0.1 1,663,556 - +0.7 1,663,566 + -2.4 1,663,566 + +0.6 1,663,654 - -0.7 1,663,665 + +0.8 1,663,666 - -0.9 1,663,666 - -0.4 1,663,666 - -1.3 1,663,678 + -0.9 1,663,678 + +0.5 1,663,679 - +0.9 1,663,679 - +0.0 1,663,713 - -1.7 1,663,732 - +0.8 1,663,953 + Psyr_1475 0.16 +0.7 1,663,954 - Psyr_1475 0.17 -0.5 1,663,954 - Psyr_1475 0.17 -1.1 1,664,148 - Psyr_1475 0.37 +1.1 1,664,244 + Psyr_1475 0.47 +1.5 1,664,244 + Psyr_1475 0.47 -0.6 1,664,244 + Psyr_1475 0.47 -0.0 1,664,244 + Psyr_1475 0.47 -1.4 1,664,245 - Psyr_1475 0.47 +1.0 1,664,245 - Psyr_1475 0.47 +0.1 1,664,245 - Psyr_1475 0.47 -0.1 1,664,245 - Psyr_1475 0.47 -1.4 1,664,245 - Psyr_1475 0.47 -0.9 1,664,245 - Psyr_1475 0.47 -0.1 1,664,245 - Psyr_1475 0.47 +0.7 1,664,350 - Psyr_1475 0.58 +0.5 1,664,392 + Psyr_1475 0.62 +1.2 1,664,424 + Psyr_1475 0.65 -1.0 1,664,425 - Psyr_1475 0.65 -1.2 1,664,435 - Psyr_1475 0.66 -0.8 1,664,499 + Psyr_1475 0.73 +1.5 1,664,499 + Psyr_1475 0.73 -0.8 1,664,499 + Psyr_1475 0.73 -0.5 1,664,500 - Psyr_1475 0.73 -1.5 1,664,500 - Psyr_1475 0.73 +0.1 1,664,500 - Psyr_1475 0.73 +0.1
Or see this region's nucleotide sequence