Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1347 and Psyr_1348 overlap by 1 nucleotides Psyr_1348 and Psyr_1349 overlap by 4 nucleotides Psyr_1349 and Psyr_1350 are separated by 117 nucleotides
Psyr_1347: Psyr_1347 - Undecaprenyl pyrophosphate synthetase, at 1,526,534 to 1,527,289
_1347
Psyr_1348: Psyr_1348 - Phosphatidate cytidylyltransferase, at 1,527,289 to 1,528,104
_1348
Psyr_1349: Psyr_1349 - 1-deoxy-D-xylulose 5-phosphate reductoisomerase, at 1,528,101 to 1,529,291
_1349
Psyr_1350: Psyr_1350 - site-2 protease, Metallo peptidase, MEROPS family M50B, at 1,529,409 to 1,530,761
_1350
Position (kb) 1528
1529
1530 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1527.267 kb on + strand at 1527.267 kb on + strand at 1529.419 kb on + strand at 1529.419 kb on + strand at 1529.419 kb on + strand at 1529.419 kb on + strand at 1529.420 kb on - strand at 1529.420 kb on - strand at 1529.420 kb on - strand at 1529.420 kb on - strand at 1529.421 kb on + strand at 1529.421 kb on + strand at 1529.421 kb on + strand at 1529.421 kb on + strand at 1529.421 kb on + strand at 1529.421 kb on + strand at 1529.421 kb on + strand at 1529.421 kb on + strand at 1529.421 kb on + strand at 1529.421 kb on + strand at 1529.421 kb on + strand at 1529.421 kb on + strand at 1529.421 kb on + strand at 1529.421 kb on + strand at 1529.421 kb on + strand at 1529.422 kb on - strand at 1529.422 kb on - strand at 1529.422 kb on - strand at 1529.422 kb on - strand at 1529.422 kb on - strand at 1529.422 kb on - strand at 1529.422 kb on - strand at 1529.422 kb on - strand at 1529.581 kb on - strand, within Psyr_1350 at 1529.581 kb on - strand, within Psyr_1350 at 1529.581 kb on - strand, within Psyr_1350 at 1529.611 kb on - strand, within Psyr_1350 at 1529.879 kb on + strand, within Psyr_1350 at 1529.970 kb on - strand, within Psyr_1350 at 1529.995 kb on + strand, within Psyr_1350
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 1,527,267 + +0.3 1,527,267 + -0.0 1,529,419 + -1.8 1,529,419 + -0.4 1,529,419 + +0.4 1,529,419 + -3.0 1,529,420 - +0.1 1,529,420 - -1.9 1,529,420 - +1.4 1,529,420 - +0.8 1,529,421 + -0.2 1,529,421 + +0.1 1,529,421 + +1.4 1,529,421 + +1.4 1,529,421 + -0.8 1,529,421 + -0.3 1,529,421 + +0.8 1,529,421 + -0.9 1,529,421 + -1.3 1,529,421 + +0.2 1,529,421 + +0.8 1,529,421 + +1.9 1,529,421 + +0.5 1,529,421 + -1.7 1,529,421 + -1.6 1,529,422 - -0.2 1,529,422 - -0.7 1,529,422 - +1.2 1,529,422 - +0.4 1,529,422 - +0.4 1,529,422 - -1.9 1,529,422 - -1.6 1,529,422 - -0.6 1,529,581 - Psyr_1350 0.13 +0.4 1,529,581 - Psyr_1350 0.13 -1.4 1,529,581 - Psyr_1350 0.13 +0.1 1,529,611 - Psyr_1350 0.15 -1.9 1,529,879 + Psyr_1350 0.35 +0.6 1,529,970 - Psyr_1350 0.41 -1.1 1,529,995 + Psyr_1350 0.43 -0.9
Or see this region's nucleotide sequence