Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1323 and Psyr_1324 are separated by 36 nucleotides Psyr_1324 and Psyr_1325 are separated by 96 nucleotides Psyr_1325 and Psyr_1326 are separated by 71 nucleotides Psyr_1326 and Psyr_1327 are separated by 204 nucleotides
Psyr_1323: Psyr_1323 - Extensin-like, C-terminal, at 1,501,040 to 1,501,729
_1323
Psyr_1324: Psyr_1324 - conserved hypothetical protein, at 1,501,766 to 1,502,389
_1324
Psyr_1325: Psyr_1325 - Protein of unknown function DUF548, at 1,502,486 to 1,503,295
_1325
Psyr_1326: Psyr_1326 - conserved hypothetical protein, at 1,503,367 to 1,504,056
_1326
Psyr_1327: Psyr_1327 - conserved hypothetical protein, at 1,504,261 to 1,504,611
_1327
Position (kb)
1502
1503
1504 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1501.572 kb on + strand, within Psyr_1323 at 1501.573 kb on - strand, within Psyr_1323 at 1501.573 kb on - strand, within Psyr_1323 at 1501.573 kb on - strand, within Psyr_1323 at 1501.648 kb on + strand, within Psyr_1323 at 1501.667 kb on + strand at 1501.667 kb on + strand at 1501.668 kb on - strand at 1501.668 kb on - strand at 1501.767 kb on + strand at 1501.767 kb on + strand at 1501.768 kb on - strand at 1502.146 kb on + strand, within Psyr_1324 at 1502.146 kb on + strand, within Psyr_1324 at 1502.146 kb on + strand, within Psyr_1324 at 1502.146 kb on + strand, within Psyr_1324 at 1502.146 kb on + strand, within Psyr_1324 at 1502.147 kb on - strand, within Psyr_1324 at 1502.147 kb on - strand, within Psyr_1324 at 1502.204 kb on + strand, within Psyr_1324 at 1502.204 kb on + strand, within Psyr_1324 at 1502.204 kb on + strand, within Psyr_1324 at 1502.205 kb on - strand, within Psyr_1324 at 1502.205 kb on - strand, within Psyr_1324 at 1502.205 kb on - strand, within Psyr_1324 at 1502.205 kb on - strand, within Psyr_1324 at 1502.205 kb on - strand, within Psyr_1324 at 1502.205 kb on - strand, within Psyr_1324 at 1502.358 kb on + strand at 1502.389 kb on + strand at 1502.389 kb on + strand at 1502.389 kb on + strand at 1502.390 kb on - strand at 1502.390 kb on - strand at 1502.399 kb on + strand at 1502.400 kb on - strand at 1502.429 kb on + strand at 1502.460 kb on + strand at 1502.479 kb on - strand at 1503.015 kb on - strand, within Psyr_1325 at 1503.015 kb on - strand, within Psyr_1325 at 1503.488 kb on + strand, within Psyr_1326 at 1503.488 kb on + strand, within Psyr_1326 at 1503.557 kb on + strand, within Psyr_1326 at 1503.557 kb on + strand, within Psyr_1326 at 1503.558 kb on - strand, within Psyr_1326 at 1503.558 kb on - strand, within Psyr_1326 at 1503.558 kb on - strand, within Psyr_1326 at 1503.558 kb on - strand, within Psyr_1326 at 1503.558 kb on - strand, within Psyr_1326 at 1503.721 kb on + strand, within Psyr_1326 at 1503.721 kb on + strand, within Psyr_1326 at 1503.721 kb on + strand, within Psyr_1326 at 1503.721 kb on + strand, within Psyr_1326 at 1503.722 kb on - strand, within Psyr_1326 at 1503.722 kb on - strand, within Psyr_1326 at 1503.862 kb on + strand, within Psyr_1326 at 1503.862 kb on + strand, within Psyr_1326 at 1503.862 kb on + strand, within Psyr_1326 at 1503.862 kb on + strand, within Psyr_1326 at 1503.862 kb on + strand, within Psyr_1326 at 1503.862 kb on + strand, within Psyr_1326 at 1503.862 kb on + strand, within Psyr_1326 at 1503.862 kb on + strand, within Psyr_1326 at 1503.863 kb on - strand, within Psyr_1326 at 1503.863 kb on - strand, within Psyr_1326 at 1503.863 kb on - strand, within Psyr_1326 at 1503.863 kb on - strand, within Psyr_1326 at 1503.863 kb on - strand, within Psyr_1326 at 1503.863 kb on - strand, within Psyr_1326 at 1503.863 kb on - strand, within Psyr_1326 at 1503.863 kb on - strand, within Psyr_1326 at 1503.863 kb on - strand, within Psyr_1326 at 1503.863 kb on - strand, within Psyr_1326 at 1503.863 kb on - strand, within Psyr_1326 at 1504.128 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 1,501,572 + Psyr_1323 0.77 +0.3 1,501,573 - Psyr_1323 0.77 +0.1 1,501,573 - Psyr_1323 0.77 +0.6 1,501,573 - Psyr_1323 0.77 +0.0 1,501,648 + Psyr_1323 0.88 -0.1 1,501,667 + +1.4 1,501,667 + -0.0 1,501,668 - +0.3 1,501,668 - +0.4 1,501,767 + -1.0 1,501,767 + -0.6 1,501,768 - +0.2 1,502,146 + Psyr_1324 0.61 +0.3 1,502,146 + Psyr_1324 0.61 -0.2 1,502,146 + Psyr_1324 0.61 +1.4 1,502,146 + Psyr_1324 0.61 +0.4 1,502,146 + Psyr_1324 0.61 -0.2 1,502,147 - Psyr_1324 0.61 -2.6 1,502,147 - Psyr_1324 0.61 +0.1 1,502,204 + Psyr_1324 0.70 -0.8 1,502,204 + Psyr_1324 0.70 +1.6 1,502,204 + Psyr_1324 0.70 +0.2 1,502,205 - Psyr_1324 0.70 -0.2 1,502,205 - Psyr_1324 0.70 -0.6 1,502,205 - Psyr_1324 0.70 +0.7 1,502,205 - Psyr_1324 0.70 +0.2 1,502,205 - Psyr_1324 0.70 +2.4 1,502,205 - Psyr_1324 0.70 -1.9 1,502,358 + -1.2 1,502,389 + +1.6 1,502,389 + +1.1 1,502,389 + +0.0 1,502,390 - -1.4 1,502,390 - +1.4 1,502,399 + +0.5 1,502,400 - -0.2 1,502,429 + -0.9 1,502,460 + +0.1 1,502,479 - -0.6 1,503,015 - Psyr_1325 0.65 -0.6 1,503,015 - Psyr_1325 0.65 -1.0 1,503,488 + Psyr_1326 0.18 -0.5 1,503,488 + Psyr_1326 0.18 -0.4 1,503,557 + Psyr_1326 0.28 -0.2 1,503,557 + Psyr_1326 0.28 +0.8 1,503,558 - Psyr_1326 0.28 -0.4 1,503,558 - Psyr_1326 0.28 +2.0 1,503,558 - Psyr_1326 0.28 -1.1 1,503,558 - Psyr_1326 0.28 +2.4 1,503,558 - Psyr_1326 0.28 +0.8 1,503,721 + Psyr_1326 0.51 +0.1 1,503,721 + Psyr_1326 0.51 -0.7 1,503,721 + Psyr_1326 0.51 -1.0 1,503,721 + Psyr_1326 0.51 +0.0 1,503,722 - Psyr_1326 0.51 -0.1 1,503,722 - Psyr_1326 0.51 +0.2 1,503,862 + Psyr_1326 0.72 -2.7 1,503,862 + Psyr_1326 0.72 -1.9 1,503,862 + Psyr_1326 0.72 +0.2 1,503,862 + Psyr_1326 0.72 +0.2 1,503,862 + Psyr_1326 0.72 -0.4 1,503,862 + Psyr_1326 0.72 -0.5 1,503,862 + Psyr_1326 0.72 -1.0 1,503,862 + Psyr_1326 0.72 +0.2 1,503,863 - Psyr_1326 0.72 +0.7 1,503,863 - Psyr_1326 0.72 +0.1 1,503,863 - Psyr_1326 0.72 +0.9 1,503,863 - Psyr_1326 0.72 -0.2 1,503,863 - Psyr_1326 0.72 -1.4 1,503,863 - Psyr_1326 0.72 +1.2 1,503,863 - Psyr_1326 0.72 +0.8 1,503,863 - Psyr_1326 0.72 -0.8 1,503,863 - Psyr_1326 0.72 -1.0 1,503,863 - Psyr_1326 0.72 +0.9 1,503,863 - Psyr_1326 0.72 +0.2 1,504,128 - +1.5
Or see this region's nucleotide sequence