Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1310 and Psyr_1311 are separated by 69 nucleotides Psyr_1311 and Psyr_1312 are separated by 91 nucleotides Psyr_1312 and Psyr_1313 are separated by 134 nucleotides
Psyr_1310: Psyr_1310 - bacterial peptide chain release factor 2 (bRF-2), at 1,487,230 to 1,488,219
_1310
Psyr_1311: Psyr_1311 - Lysyl-tRNA synthetase, class-2, at 1,488,289 to 1,489,791
_1311
Psyr_1312: Psyr_1312 - transcriptional regulator, TetR family, at 1,489,883 to 1,490,608
_1312
Psyr_1313: Psyr_1313 - conserved hypothetical protein, at 1,490,743 to 1,492,020
_1313
Position (kb)
1488
1489
1490 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1489.868 kb on + strand at 1489.868 kb on + strand at 1489.868 kb on + strand at 1489.868 kb on + strand at 1489.868 kb on + strand at 1489.868 kb on + strand at 1489.869 kb on - strand at 1489.869 kb on - strand at 1489.869 kb on - strand at 1489.869 kb on - strand at 1489.869 kb on - strand at 1489.869 kb on - strand at 1489.870 kb on + strand at 1489.871 kb on - strand at 1489.871 kb on - strand at 1489.871 kb on - strand at 1489.871 kb on - strand at 1489.943 kb on + strand at 1489.943 kb on + strand at 1489.943 kb on + strand at 1489.944 kb on - strand at 1489.944 kb on - strand at 1489.944 kb on - strand at 1489.944 kb on - strand at 1490.096 kb on + strand, within Psyr_1312 at 1490.096 kb on + strand, within Psyr_1312 at 1490.096 kb on + strand, within Psyr_1312 at 1490.097 kb on - strand, within Psyr_1312 at 1490.097 kb on - strand, within Psyr_1312 at 1490.097 kb on - strand, within Psyr_1312 at 1490.097 kb on - strand, within Psyr_1312 at 1490.097 kb on - strand, within Psyr_1312 at 1490.097 kb on - strand, within Psyr_1312 at 1490.097 kb on - strand, within Psyr_1312 at 1490.099 kb on + strand, within Psyr_1312 at 1490.099 kb on + strand, within Psyr_1312 at 1490.099 kb on + strand at 1490.099 kb on + strand, within Psyr_1312 at 1490.099 kb on + strand, within Psyr_1312 at 1490.099 kb on + strand, within Psyr_1312 at 1490.100 kb on - strand, within Psyr_1312 at 1490.100 kb on - strand, within Psyr_1312 at 1490.100 kb on - strand, within Psyr_1312 at 1490.100 kb on - strand, within Psyr_1312 at 1490.100 kb on - strand, within Psyr_1312 at 1490.100 kb on - strand, within Psyr_1312 at 1490.100 kb on - strand, within Psyr_1312 at 1490.100 kb on - strand, within Psyr_1312 at 1490.100 kb on - strand, within Psyr_1312 at 1490.144 kb on + strand, within Psyr_1312 at 1490.144 kb on + strand, within Psyr_1312 at 1490.144 kb on + strand, within Psyr_1312 at 1490.144 kb on + strand, within Psyr_1312 at 1490.144 kb on + strand, within Psyr_1312 at 1490.144 kb on + strand, within Psyr_1312 at 1490.145 kb on - strand, within Psyr_1312 at 1490.145 kb on - strand, within Psyr_1312 at 1490.145 kb on - strand, within Psyr_1312 at 1490.145 kb on - strand, within Psyr_1312 at 1490.145 kb on - strand, within Psyr_1312 at 1490.145 kb on - strand, within Psyr_1312 at 1490.145 kb on - strand, within Psyr_1312 at 1490.145 kb on - strand, within Psyr_1312 at 1490.148 kb on + strand, within Psyr_1312 at 1490.148 kb on + strand, within Psyr_1312 at 1490.148 kb on + strand, within Psyr_1312 at 1490.148 kb on + strand, within Psyr_1312 at 1490.148 kb on + strand, within Psyr_1312 at 1490.149 kb on - strand, within Psyr_1312 at 1490.149 kb on - strand, within Psyr_1312 at 1490.149 kb on - strand, within Psyr_1312 at 1490.149 kb on - strand, within Psyr_1312 at 1490.149 kb on - strand, within Psyr_1312 at 1490.197 kb on + strand, within Psyr_1312 at 1490.197 kb on + strand, within Psyr_1312 at 1490.197 kb on + strand, within Psyr_1312 at 1490.198 kb on - strand, within Psyr_1312 at 1490.198 kb on - strand, within Psyr_1312 at 1490.198 kb on - strand, within Psyr_1312 at 1490.198 kb on - strand, within Psyr_1312 at 1490.272 kb on + strand, within Psyr_1312 at 1490.273 kb on - strand, within Psyr_1312 at 1490.273 kb on - strand, within Psyr_1312 at 1490.288 kb on - strand, within Psyr_1312 at 1490.318 kb on + strand, within Psyr_1312 at 1490.318 kb on + strand, within Psyr_1312 at 1490.319 kb on - strand, within Psyr_1312 at 1490.342 kb on + strand, within Psyr_1312 at 1490.342 kb on + strand, within Psyr_1312 at 1490.343 kb on - strand, within Psyr_1312 at 1490.343 kb on - strand, within Psyr_1312 at 1490.343 kb on - strand, within Psyr_1312 at 1490.343 kb on - strand, within Psyr_1312 at 1490.434 kb on - strand, within Psyr_1312 at 1490.499 kb on - strand, within Psyr_1312 at 1490.610 kb on + strand at 1490.610 kb on + strand at 1490.610 kb on + strand at 1490.610 kb on + strand at 1490.611 kb on - strand at 1490.611 kb on - strand at 1490.611 kb on - strand at 1490.611 kb on - strand at 1490.611 kb on - strand at 1490.612 kb on + strand at 1490.612 kb on + strand at 1490.612 kb on + strand at 1490.612 kb on + strand at 1490.612 kb on + strand at 1490.612 kb on + strand at 1490.613 kb on - strand at 1490.613 kb on - strand at 1490.613 kb on - strand at 1490.613 kb on - strand at 1490.675 kb on - strand at 1490.675 kb on - strand at 1490.678 kb on + strand at 1490.752 kb on + strand at 1490.752 kb on + strand at 1490.763 kb on + strand at 1490.763 kb on + strand at 1490.764 kb on - strand at 1490.764 kb on - strand at 1490.764 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 1,489,868 + -0.2 1,489,868 + -0.2 1,489,868 + -0.6 1,489,868 + -0.0 1,489,868 + +0.1 1,489,868 + -0.2 1,489,869 - -0.0 1,489,869 - -0.5 1,489,869 - -0.1 1,489,869 - -0.5 1,489,869 - -0.2 1,489,869 - +0.7 1,489,870 + +0.5 1,489,871 - +0.6 1,489,871 - -0.3 1,489,871 - +0.7 1,489,871 - -0.7 1,489,943 + -0.1 1,489,943 + +1.7 1,489,943 + +0.1 1,489,944 - -0.3 1,489,944 - +0.1 1,489,944 - -0.4 1,489,944 - -0.6 1,490,096 + Psyr_1312 0.29 +0.7 1,490,096 + Psyr_1312 0.29 +0.2 1,490,096 + Psyr_1312 0.29 -1.3 1,490,097 - Psyr_1312 0.29 +0.4 1,490,097 - Psyr_1312 0.29 +0.7 1,490,097 - Psyr_1312 0.29 -0.7 1,490,097 - Psyr_1312 0.29 -0.3 1,490,097 - Psyr_1312 0.29 -1.0 1,490,097 - Psyr_1312 0.29 -0.7 1,490,097 - Psyr_1312 0.29 -0.3 1,490,099 + Psyr_1312 0.30 -0.3 1,490,099 + Psyr_1312 0.30 -0.1 1,490,099 + +1.4 1,490,099 + Psyr_1312 0.30 -0.5 1,490,099 + Psyr_1312 0.30 -0.1 1,490,099 + Psyr_1312 0.30 -0.1 1,490,100 - Psyr_1312 0.30 -0.7 1,490,100 - Psyr_1312 0.30 -1.2 1,490,100 - Psyr_1312 0.30 -1.3 1,490,100 - Psyr_1312 0.30 -0.6 1,490,100 - Psyr_1312 0.30 +0.8 1,490,100 - Psyr_1312 0.30 -0.0 1,490,100 - Psyr_1312 0.30 +0.4 1,490,100 - Psyr_1312 0.30 -1.4 1,490,100 - Psyr_1312 0.30 +0.9 1,490,144 + Psyr_1312 0.36 -0.4 1,490,144 + Psyr_1312 0.36 +0.4 1,490,144 + Psyr_1312 0.36 +0.3 1,490,144 + Psyr_1312 0.36 -0.4 1,490,144 + Psyr_1312 0.36 +0.2 1,490,144 + Psyr_1312 0.36 -0.8 1,490,145 - Psyr_1312 0.36 -1.2 1,490,145 - Psyr_1312 0.36 -0.1 1,490,145 - Psyr_1312 0.36 -0.3 1,490,145 - Psyr_1312 0.36 +2.3 1,490,145 - Psyr_1312 0.36 -0.3 1,490,145 - Psyr_1312 0.36 +1.1 1,490,145 - Psyr_1312 0.36 +0.0 1,490,145 - Psyr_1312 0.36 -0.3 1,490,148 + Psyr_1312 0.37 +0.8 1,490,148 + Psyr_1312 0.37 -1.2 1,490,148 + Psyr_1312 0.37 -0.3 1,490,148 + Psyr_1312 0.37 -0.2 1,490,148 + Psyr_1312 0.37 -0.9 1,490,149 - Psyr_1312 0.37 -0.3 1,490,149 - Psyr_1312 0.37 +0.7 1,490,149 - Psyr_1312 0.37 -0.8 1,490,149 - Psyr_1312 0.37 -1.0 1,490,149 - Psyr_1312 0.37 -2.2 1,490,197 + Psyr_1312 0.43 +0.9 1,490,197 + Psyr_1312 0.43 +0.7 1,490,197 + Psyr_1312 0.43 -1.2 1,490,198 - Psyr_1312 0.43 +0.3 1,490,198 - Psyr_1312 0.43 +0.4 1,490,198 - Psyr_1312 0.43 +1.0 1,490,198 - Psyr_1312 0.43 +0.5 1,490,272 + Psyr_1312 0.54 -0.1 1,490,273 - Psyr_1312 0.54 +1.2 1,490,273 - Psyr_1312 0.54 -0.4 1,490,288 - Psyr_1312 0.56 +1.7 1,490,318 + Psyr_1312 0.60 +0.3 1,490,318 + Psyr_1312 0.60 -0.4 1,490,319 - Psyr_1312 0.60 -0.6 1,490,342 + Psyr_1312 0.63 +1.4 1,490,342 + Psyr_1312 0.63 +0.3 1,490,343 - Psyr_1312 0.63 -0.3 1,490,343 - Psyr_1312 0.63 +0.0 1,490,343 - Psyr_1312 0.63 -0.4 1,490,343 - Psyr_1312 0.63 -2.3 1,490,434 - Psyr_1312 0.76 +1.2 1,490,499 - Psyr_1312 0.85 +0.8 1,490,610 + +0.1 1,490,610 + +0.7 1,490,610 + +1.2 1,490,610 + +0.3 1,490,611 - -0.4 1,490,611 - +0.8 1,490,611 - +1.0 1,490,611 - +0.4 1,490,611 - -0.6 1,490,612 + -0.4 1,490,612 + +1.6 1,490,612 + +0.0 1,490,612 + +0.4 1,490,612 + +0.3 1,490,612 + +0.1 1,490,613 - +0.7 1,490,613 - -2.4 1,490,613 - +0.4 1,490,613 - +0.5 1,490,675 - -2.5 1,490,675 - +0.3 1,490,678 + +0.4 1,490,752 + -0.6 1,490,752 + -0.4 1,490,763 + -0.7 1,490,763 + +0.2 1,490,764 - -1.8 1,490,764 - +1.0 1,490,764 - +2.7
Or see this region's nucleotide sequence