Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1283 and Psyr_1284 are separated by 5 nucleotides Psyr_1284 and Psyr_1285 are separated by 44 nucleotides Psyr_1285 and Psyr_1286 are separated by 103 nucleotides
Psyr_1283: Psyr_1283 - 16S rRNA processing protein RimM, at 1,449,236 to 1,449,775
_1283
Psyr_1284: Psyr_1284 - tRNA (Guanine37-N(1)-) methyltransferase, at 1,449,781 to 1,450,533
_1284
Psyr_1285: Psyr_1285 - LSU ribosomal protein L19P, at 1,450,578 to 1,450,928
_1285
Psyr_1286: Psyr_1286 - tyrosine recombinase XerD subunit, at 1,451,032 to 1,451,928
_1286
Position (kb) 1450
1451 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 1450.876 kb on - strand, within Psyr_1285 at 1451.028 kb on - strand at 1451.028 kb on - strand at 1451.233 kb on + strand, within Psyr_1286 at 1451.233 kb on + strand, within Psyr_1286 at 1451.233 kb on + strand, within Psyr_1286 at 1451.234 kb on - strand, within Psyr_1286 at 1451.234 kb on - strand, within Psyr_1286 at 1451.234 kb on - strand, within Psyr_1286 at 1451.236 kb on - strand, within Psyr_1286 at 1451.236 kb on - strand, within Psyr_1286 at 1451.281 kb on + strand, within Psyr_1286 at 1451.282 kb on - strand, within Psyr_1286 at 1451.287 kb on + strand, within Psyr_1286 at 1451.287 kb on + strand, within Psyr_1286 at 1451.287 kb on + strand, within Psyr_1286 at 1451.461 kb on + strand, within Psyr_1286 at 1451.461 kb on + strand, within Psyr_1286 at 1451.461 kb on + strand, within Psyr_1286 at 1451.461 kb on + strand, within Psyr_1286 at 1451.461 kb on + strand, within Psyr_1286 at 1451.461 kb on + strand, within Psyr_1286 at 1451.461 kb on + strand, within Psyr_1286 at 1451.462 kb on - strand, within Psyr_1286 at 1451.462 kb on - strand, within Psyr_1286 at 1451.462 kb on - strand, within Psyr_1286 at 1451.462 kb on - strand, within Psyr_1286 at 1451.608 kb on + strand, within Psyr_1286 at 1451.866 kb on + strand at 1451.866 kb on + strand at 1451.866 kb on + strand at 1451.866 kb on + strand at 1451.866 kb on + strand at 1451.866 kb on + strand at 1451.866 kb on + strand at 1451.867 kb on - strand at 1451.867 kb on - strand at 1451.867 kb on - strand at 1451.867 kb on - strand at 1451.867 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 1,450,876 - Psyr_1285 0.85 +3.4 1,451,028 - +0.1 1,451,028 - +1.2 1,451,233 + Psyr_1286 0.22 +0.9 1,451,233 + Psyr_1286 0.22 -0.4 1,451,233 + Psyr_1286 0.22 +1.4 1,451,234 - Psyr_1286 0.23 -0.0 1,451,234 - Psyr_1286 0.23 -1.0 1,451,234 - Psyr_1286 0.23 +0.8 1,451,236 - Psyr_1286 0.23 +0.2 1,451,236 - Psyr_1286 0.23 -0.6 1,451,281 + Psyr_1286 0.28 -0.3 1,451,282 - Psyr_1286 0.28 -0.2 1,451,287 + Psyr_1286 0.28 +1.1 1,451,287 + Psyr_1286 0.28 -0.5 1,451,287 + Psyr_1286 0.28 -0.5 1,451,461 + Psyr_1286 0.48 -0.3 1,451,461 + Psyr_1286 0.48 -1.5 1,451,461 + Psyr_1286 0.48 +0.2 1,451,461 + Psyr_1286 0.48 -1.1 1,451,461 + Psyr_1286 0.48 +0.1 1,451,461 + Psyr_1286 0.48 -0.2 1,451,461 + Psyr_1286 0.48 +0.9 1,451,462 - Psyr_1286 0.48 -0.1 1,451,462 - Psyr_1286 0.48 -1.2 1,451,462 - Psyr_1286 0.48 -0.2 1,451,462 - Psyr_1286 0.48 -0.1 1,451,608 + Psyr_1286 0.64 +0.5 1,451,866 + -0.6 1,451,866 + +0.1 1,451,866 + -2.2 1,451,866 + -0.2 1,451,866 + -1.8 1,451,866 + +0.2 1,451,866 + +0.1 1,451,867 - +0.2 1,451,867 - +4.0 1,451,867 - -1.5 1,451,867 - +0.9 1,451,867 - +0.0
Or see this region's nucleotide sequence