Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1247 and Psyr_1248 are separated by 3 nucleotides Psyr_1248 and Psyr_1249 are separated by 22 nucleotides
Psyr_1247: Psyr_1247 - conserved hypothetical protein, at 1,402,337 to 1,403,341
_1247
Psyr_1248: Psyr_1248 - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase, at 1,403,345 to 1,404,454
_1248
Psyr_1249: Psyr_1249 - histidyl-tRNA synthetase, at 1,404,477 to 1,405,766
_1249
Position (kb)
1403
1404
1405 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1402.362 kb on + strand at 1402.362 kb on + strand at 1402.513 kb on + strand, within Psyr_1247 at 1402.513 kb on + strand, within Psyr_1247 at 1402.529 kb on + strand, within Psyr_1247 at 1402.672 kb on + strand, within Psyr_1247 at 1402.672 kb on + strand, within Psyr_1247 at 1402.672 kb on + strand, within Psyr_1247 at 1403.118 kb on + strand, within Psyr_1247 at 1403.270 kb on + strand at 1403.270 kb on + strand at 1403.270 kb on + strand at 1403.292 kb on + strand at 1403.292 kb on + strand at 1403.292 kb on + strand at 1403.292 kb on + strand at 1403.292 kb on + strand at 1403.331 kb on + strand at 1404.483 kb on - strand at 1404.602 kb on + strand at 1404.678 kb on + strand, within Psyr_1249 at 1404.678 kb on + strand, within Psyr_1249 at 1404.678 kb on + strand, within Psyr_1249 at 1404.678 kb on + strand, within Psyr_1249 at 1404.678 kb on + strand, within Psyr_1249 at 1404.678 kb on + strand, within Psyr_1249 at 1404.678 kb on + strand, within Psyr_1249 at 1404.678 kb on + strand, within Psyr_1249 at 1404.798 kb on + strand, within Psyr_1249 at 1404.798 kb on + strand, within Psyr_1249 at 1404.798 kb on + strand, within Psyr_1249 at 1405.230 kb on + strand, within Psyr_1249 at 1405.230 kb on + strand, within Psyr_1249 at 1405.230 kb on + strand, within Psyr_1249 at 1405.230 kb on + strand, within Psyr_1249 at 1405.266 kb on + strand, within Psyr_1249 at 1405.269 kb on + strand, within Psyr_1249
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 1,402,362 + +0.8 1,402,362 + -0.2 1,402,513 + Psyr_1247 0.18 -1.4 1,402,513 + Psyr_1247 0.18 -0.9 1,402,529 + Psyr_1247 0.19 -0.2 1,402,672 + Psyr_1247 0.33 -1.3 1,402,672 + Psyr_1247 0.33 -1.9 1,402,672 + Psyr_1247 0.33 -2.3 1,403,118 + Psyr_1247 0.78 -0.8 1,403,270 + -0.3 1,403,270 + +0.5 1,403,270 + +0.0 1,403,292 + +1.2 1,403,292 + -0.8 1,403,292 + +0.3 1,403,292 + -0.2 1,403,292 + -0.1 1,403,331 + -0.4 1,404,483 - -0.9 1,404,602 + -1.5 1,404,678 + Psyr_1249 0.16 +1.4 1,404,678 + Psyr_1249 0.16 -0.8 1,404,678 + Psyr_1249 0.16 +0.5 1,404,678 + Psyr_1249 0.16 +0.9 1,404,678 + Psyr_1249 0.16 -0.6 1,404,678 + Psyr_1249 0.16 +0.9 1,404,678 + Psyr_1249 0.16 +1.2 1,404,678 + Psyr_1249 0.16 +2.4 1,404,798 + Psyr_1249 0.25 -0.3 1,404,798 + Psyr_1249 0.25 +0.2 1,404,798 + Psyr_1249 0.25 -0.1 1,405,230 + Psyr_1249 0.58 +0.9 1,405,230 + Psyr_1249 0.58 -0.3 1,405,230 + Psyr_1249 0.58 +0.6 1,405,230 + Psyr_1249 0.58 -0.4 1,405,266 + Psyr_1249 0.61 -1.8 1,405,269 + Psyr_1249 0.61 +1.2
Or see this region's nucleotide sequence