Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1228 and Psyr_1229 are separated by 53 nucleotides Psyr_1229 and Psyr_1230 are separated by 63 nucleotides Psyr_1230 and Psyr_1231 are separated by 9 nucleotides
Psyr_1228: Psyr_1228 - tRNA-guanine transglycosylase, at 1,386,415 to 1,387,548
_1228
Psyr_1229: Psyr_1229 - protein translocase subunit yajC, at 1,387,602 to 1,387,937
_1229
Psyr_1230: Psyr_1230 - SecD/SecF/SecDF export membrane protein:SecD export membrane protein, at 1,388,001 to 1,389,869
_1230
Psyr_1231: Psyr_1231 - protein translocase subunit secF, at 1,389,879 to 1,390,790
_1231
Position (kb) 1388
1389
1390 Strain fitness (log2 ratio) -2
-1
0
1 at 1387.416 kb on + strand, within Psyr_1228 at 1387.482 kb on + strand at 1387.574 kb on + strand at 1387.579 kb on + strand at 1387.579 kb on + strand at 1387.579 kb on + strand at 1387.579 kb on + strand at 1387.579 kb on + strand at 1387.579 kb on + strand at 1387.583 kb on + strand at 1387.583 kb on + strand at 1387.583 kb on + strand at 1387.583 kb on + strand at 1387.583 kb on + strand at 1387.583 kb on + strand at 1387.583 kb on + strand at 1387.583 kb on + strand at 1387.583 kb on + strand at 1387.583 kb on + strand at 1387.583 kb on + strand at 1387.583 kb on + strand at 1387.587 kb on + strand at 1387.710 kb on + strand, within Psyr_1229 at 1387.710 kb on + strand, within Psyr_1229 at 1389.834 kb on + strand at 1390.789 kb on - strand at 1390.803 kb on + strand at 1390.803 kb on + strand at 1390.803 kb on + strand at 1390.804 kb on - strand at 1390.804 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 1,387,416 + Psyr_1228 0.88 -1.4 1,387,482 + +0.0 1,387,574 + -0.1 1,387,579 + +0.2 1,387,579 + -0.9 1,387,579 + +1.0 1,387,579 + +0.2 1,387,579 + -0.1 1,387,579 + +0.1 1,387,583 + -0.2 1,387,583 + -1.2 1,387,583 + +0.6 1,387,583 + +0.7 1,387,583 + -0.8 1,387,583 + -1.0 1,387,583 + +0.5 1,387,583 + +0.6 1,387,583 + -0.7 1,387,583 + +0.2 1,387,583 + +1.4 1,387,583 + +0.0 1,387,587 + +0.2 1,387,710 + Psyr_1229 0.32 -1.6 1,387,710 + Psyr_1229 0.32 +0.4 1,389,834 + -1.9 1,390,789 - -1.0 1,390,803 + +1.2 1,390,803 + +0.8 1,390,803 + -0.1 1,390,804 - -0.7 1,390,804 - -1.8
Or see this region's nucleotide sequence