Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1193 and Psyr_1194 are separated by 27 nucleotides Psyr_1194 and Psyr_1195 are separated by 8 nucleotides Psyr_1195 and Psyr_1196 overlap by 1 nucleotides
Psyr_1193: Psyr_1193 - type III helper protein HrpZ1, at 1,353,667 to 1,354,698
_1193
Psyr_1194: Psyr_1194 - type III secretion protein HrpB, at 1,354,726 to 1,355,100
_1194
Psyr_1195: Psyr_1195 - type III secretion apparatus lipoprotein, YscJ/HrcJ family, at 1,355,109 to 1,355,915
_1195
Psyr_1196: Psyr_1196 - type III secretion protein HrpD, at 1,355,915 to 1,356,442
_1196
Position (kb)
1354
1355
1356 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1354.093 kb on - strand, within Psyr_1193 at 1354.398 kb on + strand, within Psyr_1193 at 1354.398 kb on + strand, within Psyr_1193 at 1354.398 kb on + strand, within Psyr_1193 at 1354.659 kb on + strand at 1354.659 kb on + strand at 1354.659 kb on + strand at 1354.660 kb on - strand at 1354.752 kb on + strand at 1354.752 kb on + strand at 1354.752 kb on + strand at 1354.753 kb on - strand at 1354.888 kb on - strand, within Psyr_1194 at 1355.088 kb on + strand at 1355.099 kb on - strand at 1355.149 kb on + strand at 1355.150 kb on - strand at 1355.243 kb on + strand, within Psyr_1195 at 1355.243 kb on + strand, within Psyr_1195 at 1355.424 kb on + strand, within Psyr_1195 at 1355.430 kb on + strand, within Psyr_1195 at 1355.430 kb on + strand, within Psyr_1195 at 1355.430 kb on + strand, within Psyr_1195 at 1355.430 kb on + strand, within Psyr_1195 at 1355.430 kb on + strand, within Psyr_1195 at 1355.430 kb on + strand, within Psyr_1195 at 1355.430 kb on + strand, within Psyr_1195 at 1355.430 kb on + strand, within Psyr_1195 at 1355.430 kb on + strand, within Psyr_1195 at 1355.430 kb on + strand, within Psyr_1195 at 1355.430 kb on + strand, within Psyr_1195 at 1355.430 kb on + strand, within Psyr_1195 at 1355.431 kb on - strand, within Psyr_1195 at 1355.431 kb on - strand, within Psyr_1195 at 1355.431 kb on - strand, within Psyr_1195 at 1355.431 kb on - strand, within Psyr_1195 at 1355.431 kb on - strand, within Psyr_1195 at 1355.431 kb on - strand, within Psyr_1195 at 1355.431 kb on - strand, within Psyr_1195 at 1355.431 kb on - strand, within Psyr_1195 at 1355.431 kb on - strand, within Psyr_1195 at 1355.431 kb on - strand, within Psyr_1195 at 1355.431 kb on - strand, within Psyr_1195 at 1355.431 kb on - strand, within Psyr_1195 at 1355.431 kb on - strand, within Psyr_1195 at 1355.431 kb on - strand, within Psyr_1195 at 1355.431 kb on - strand, within Psyr_1195 at 1355.431 kb on - strand, within Psyr_1195 at 1355.431 kb on - strand, within Psyr_1195 at 1355.431 kb on - strand, within Psyr_1195 at 1355.431 kb on - strand, within Psyr_1195 at 1355.470 kb on + strand, within Psyr_1195 at 1355.471 kb on - strand, within Psyr_1195 at 1355.503 kb on + strand, within Psyr_1195 at 1355.503 kb on + strand, within Psyr_1195 at 1355.503 kb on + strand, within Psyr_1195 at 1355.504 kb on - strand, within Psyr_1195 at 1355.936 kb on + strand at 1355.937 kb on - strand at 1355.937 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 1,354,093 - Psyr_1193 0.41 -0.1 1,354,398 + Psyr_1193 0.71 +0.2 1,354,398 + Psyr_1193 0.71 +2.0 1,354,398 + Psyr_1193 0.71 -0.9 1,354,659 + -0.6 1,354,659 + +0.1 1,354,659 + -0.8 1,354,660 - +0.8 1,354,752 + +1.0 1,354,752 + -0.5 1,354,752 + -1.8 1,354,753 - -0.2 1,354,888 - Psyr_1194 0.43 +0.1 1,355,088 + -0.3 1,355,099 - -0.9 1,355,149 + +1.1 1,355,150 - +0.4 1,355,243 + Psyr_1195 0.17 -0.4 1,355,243 + Psyr_1195 0.17 +0.5 1,355,424 + Psyr_1195 0.39 +0.4 1,355,430 + Psyr_1195 0.40 -0.7 1,355,430 + Psyr_1195 0.40 -1.5 1,355,430 + Psyr_1195 0.40 -0.2 1,355,430 + Psyr_1195 0.40 -0.3 1,355,430 + Psyr_1195 0.40 -0.1 1,355,430 + Psyr_1195 0.40 +0.7 1,355,430 + Psyr_1195 0.40 +0.9 1,355,430 + Psyr_1195 0.40 -1.1 1,355,430 + Psyr_1195 0.40 -0.4 1,355,430 + Psyr_1195 0.40 +0.7 1,355,430 + Psyr_1195 0.40 -0.0 1,355,430 + Psyr_1195 0.40 -1.8 1,355,431 - Psyr_1195 0.40 +0.5 1,355,431 - Psyr_1195 0.40 +1.1 1,355,431 - Psyr_1195 0.40 +1.1 1,355,431 - Psyr_1195 0.40 +0.1 1,355,431 - Psyr_1195 0.40 +0.0 1,355,431 - Psyr_1195 0.40 -1.0 1,355,431 - Psyr_1195 0.40 +0.6 1,355,431 - Psyr_1195 0.40 +0.4 1,355,431 - Psyr_1195 0.40 -0.0 1,355,431 - Psyr_1195 0.40 -0.4 1,355,431 - Psyr_1195 0.40 +0.2 1,355,431 - Psyr_1195 0.40 +0.1 1,355,431 - Psyr_1195 0.40 +0.7 1,355,431 - Psyr_1195 0.40 +0.2 1,355,431 - Psyr_1195 0.40 -0.3 1,355,431 - Psyr_1195 0.40 -0.2 1,355,431 - Psyr_1195 0.40 +0.6 1,355,431 - Psyr_1195 0.40 -1.9 1,355,431 - Psyr_1195 0.40 +0.3 1,355,470 + Psyr_1195 0.45 +0.8 1,355,471 - Psyr_1195 0.45 +0.5 1,355,503 + Psyr_1195 0.49 -0.1 1,355,503 + Psyr_1195 0.49 +0.2 1,355,503 + Psyr_1195 0.49 -0.4 1,355,504 - Psyr_1195 0.49 +0.7 1,355,936 + +0.3 1,355,937 - +0.4 1,355,937 - +0.1
Or see this region's nucleotide sequence