Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0966 and Psyr_0967 are separated by 27 nucleotides Psyr_0967 and Psyr_0968 are separated by 177 nucleotides
Psyr_0966: Psyr_0966 - Conserved hypothetical protein YfcH, at 1,099,569 to 1,100,471
_0966
Psyr_0967: Psyr_0967 - ferrochelatase, at 1,100,499 to 1,101,521
_0967
Psyr_0968: Psyr_0968 - Xanthine/uracil permease family, at 1,101,699 to 1,102,976
_0968
Position (kb)
1100
1101
1102 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1099.525 kb on + strand at 1099.525 kb on + strand at 1099.526 kb on - strand at 1099.526 kb on - strand at 1099.526 kb on - strand at 1099.526 kb on - strand at 1099.531 kb on + strand at 1099.531 kb on + strand at 1099.532 kb on - strand at 1099.532 kb on - strand at 1099.546 kb on + strand at 1099.546 kb on + strand at 1099.546 kb on + strand at 1099.547 kb on - strand at 1099.547 kb on - strand at 1099.547 kb on - strand at 1099.547 kb on - strand at 1099.547 kb on - strand at 1099.576 kb on + strand at 1099.576 kb on + strand at 1099.576 kb on + strand at 1099.576 kb on + strand at 1099.576 kb on + strand at 1099.576 kb on + strand at 1099.576 kb on + strand at 1099.576 kb on + strand at 1099.577 kb on - strand at 1099.577 kb on - strand at 1099.577 kb on - strand at 1099.577 kb on - strand at 1099.577 kb on - strand at 1099.577 kb on - strand at 1099.577 kb on - strand at 1099.577 kb on - strand at 1099.592 kb on + strand at 1099.593 kb on - strand at 1099.655 kb on - strand at 1100.050 kb on + strand, within Psyr_0966 at 1100.203 kb on + strand, within Psyr_0966 at 1100.229 kb on + strand, within Psyr_0966 at 1100.229 kb on + strand, within Psyr_0966 at 1100.229 kb on + strand, within Psyr_0966 at 1100.229 kb on + strand, within Psyr_0966 at 1100.229 kb on + strand, within Psyr_0966 at 1100.229 kb on + strand, within Psyr_0966 at 1100.229 kb on + strand, within Psyr_0966 at 1100.229 kb on + strand, within Psyr_0966 at 1100.229 kb on + strand, within Psyr_0966 at 1100.229 kb on + strand, within Psyr_0966 at 1100.230 kb on - strand, within Psyr_0966 at 1100.230 kb on - strand, within Psyr_0966 at 1100.231 kb on + strand, within Psyr_0966 at 1100.231 kb on + strand, within Psyr_0966 at 1100.231 kb on + strand, within Psyr_0966 at 1100.231 kb on + strand, within Psyr_0966 at 1100.232 kb on - strand, within Psyr_0966 at 1100.232 kb on - strand, within Psyr_0966 at 1100.338 kb on + strand, within Psyr_0966 at 1100.338 kb on + strand, within Psyr_0966 at 1100.338 kb on + strand, within Psyr_0966 at 1100.414 kb on + strand at 1100.414 kb on + strand at 1100.414 kb on + strand at 1100.424 kb on + strand at 1100.424 kb on + strand at 1100.425 kb on - strand at 1100.488 kb on + strand at 1100.488 kb on + strand at 1100.489 kb on - strand at 1100.489 kb on - strand at 1101.519 kb on + strand at 1101.519 kb on + strand at 1101.519 kb on + strand at 1101.519 kb on + strand at 1101.519 kb on + strand at 1101.519 kb on + strand at 1101.519 kb on + strand at 1101.519 kb on + strand at 1101.520 kb on - strand at 1101.520 kb on - strand at 1101.520 kb on - strand at 1101.520 kb on - strand at 1101.555 kb on + strand at 1101.840 kb on + strand, within Psyr_0968 at 1101.841 kb on - strand, within Psyr_0968 at 1102.078 kb on + strand, within Psyr_0968 at 1102.078 kb on + strand, within Psyr_0968 at 1102.078 kb on + strand, within Psyr_0968 at 1102.078 kb on + strand, within Psyr_0968 at 1102.078 kb on + strand, within Psyr_0968 at 1102.078 kb on + strand, within Psyr_0968 at 1102.079 kb on - strand, within Psyr_0968 at 1102.079 kb on - strand, within Psyr_0968 at 1102.117 kb on + strand, within Psyr_0968 at 1102.118 kb on - strand, within Psyr_0968 at 1102.118 kb on - strand, within Psyr_0968 at 1102.124 kb on + strand, within Psyr_0968 at 1102.387 kb on + strand, within Psyr_0968 at 1102.388 kb on - strand, within Psyr_0968 at 1102.388 kb on - strand, within Psyr_0968 at 1102.388 kb on - strand, within Psyr_0968 at 1102.388 kb on - strand, within Psyr_0968 at 1102.388 kb on - strand, within Psyr_0968
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 1,099,525 + +0.2 1,099,525 + -1.2 1,099,526 - -0.9 1,099,526 - -0.5 1,099,526 - +1.4 1,099,526 - -0.0 1,099,531 + +0.7 1,099,531 + -0.1 1,099,532 - +0.4 1,099,532 - -1.0 1,099,546 + -0.4 1,099,546 + +0.8 1,099,546 + -0.5 1,099,547 - -0.5 1,099,547 - -0.3 1,099,547 - -0.3 1,099,547 - -0.2 1,099,547 - +0.5 1,099,576 + +0.1 1,099,576 + +1.1 1,099,576 + -0.6 1,099,576 + +0.7 1,099,576 + -0.6 1,099,576 + -0.1 1,099,576 + +0.4 1,099,576 + -0.3 1,099,577 - -0.3 1,099,577 - +0.4 1,099,577 - -0.9 1,099,577 - -0.3 1,099,577 - -0.6 1,099,577 - -0.1 1,099,577 - -0.0 1,099,577 - +0.4 1,099,592 + +0.0 1,099,593 - -0.4 1,099,655 - -0.2 1,100,050 + Psyr_0966 0.53 -0.5 1,100,203 + Psyr_0966 0.70 -1.2 1,100,229 + Psyr_0966 0.73 -1.4 1,100,229 + Psyr_0966 0.73 +0.5 1,100,229 + Psyr_0966 0.73 -0.1 1,100,229 + Psyr_0966 0.73 -1.9 1,100,229 + Psyr_0966 0.73 -0.3 1,100,229 + Psyr_0966 0.73 -1.3 1,100,229 + Psyr_0966 0.73 -0.5 1,100,229 + Psyr_0966 0.73 +1.6 1,100,229 + Psyr_0966 0.73 +0.7 1,100,229 + Psyr_0966 0.73 +0.9 1,100,230 - Psyr_0966 0.73 -1.1 1,100,230 - Psyr_0966 0.73 -1.9 1,100,231 + Psyr_0966 0.73 +0.4 1,100,231 + Psyr_0966 0.73 +1.2 1,100,231 + Psyr_0966 0.73 -0.7 1,100,231 + Psyr_0966 0.73 +0.4 1,100,232 - Psyr_0966 0.73 +1.3 1,100,232 - Psyr_0966 0.73 +0.3 1,100,338 + Psyr_0966 0.85 -0.1 1,100,338 + Psyr_0966 0.85 -1.6 1,100,338 + Psyr_0966 0.85 -0.4 1,100,414 + -1.1 1,100,414 + +0.8 1,100,414 + +0.6 1,100,424 + -1.4 1,100,424 + +0.0 1,100,425 - +0.4 1,100,488 + +0.6 1,100,488 + -0.3 1,100,489 - -0.2 1,100,489 - -2.9 1,101,519 + +0.5 1,101,519 + +0.0 1,101,519 + +0.1 1,101,519 + -1.1 1,101,519 + +1.9 1,101,519 + -0.2 1,101,519 + -0.2 1,101,519 + +0.6 1,101,520 - +0.1 1,101,520 - -0.3 1,101,520 - -2.1 1,101,520 - -0.4 1,101,555 + -0.2 1,101,840 + Psyr_0968 0.11 +0.6 1,101,841 - Psyr_0968 0.11 -0.0 1,102,078 + Psyr_0968 0.30 -1.0 1,102,078 + Psyr_0968 0.30 +0.7 1,102,078 + Psyr_0968 0.30 -0.6 1,102,078 + Psyr_0968 0.30 +0.2 1,102,078 + Psyr_0968 0.30 +3.0 1,102,078 + Psyr_0968 0.30 +0.7 1,102,079 - Psyr_0968 0.30 +0.7 1,102,079 - Psyr_0968 0.30 -0.4 1,102,117 + Psyr_0968 0.33 -0.9 1,102,118 - Psyr_0968 0.33 -1.4 1,102,118 - Psyr_0968 0.33 -0.2 1,102,124 + Psyr_0968 0.33 -0.6 1,102,387 + Psyr_0968 0.54 -1.1 1,102,388 - Psyr_0968 0.54 +0.2 1,102,388 - Psyr_0968 0.54 +0.7 1,102,388 - Psyr_0968 0.54 +1.4 1,102,388 - Psyr_0968 0.54 -0.3 1,102,388 - Psyr_0968 0.54 -0.4
Or see this region's nucleotide sequence