Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0944 and Psyr_RNA19 are separated by 45 nucleotides Psyr_RNA19 and Psyr_0945 are separated by 37 nucleotides Psyr_0945 and Psyr_0946 are separated by 2 nucleotides Psyr_0946 and Psyr_0947 are separated by 4 nucleotides
Psyr_0944: Psyr_0944 - Ribose-phosphate pyrophosphokinase, at 1,079,101 to 1,080,042
_0944
Psyr_RNA19: Psyr_RNA19 - tRNA-Gln, at 1,080,088 to 1,080,162
_RNA19
Psyr_0945: Psyr_0945 - 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, at 1,080,200 to 1,081,048
_0945
Psyr_0946: Psyr_0946 - Outer membrane lipoprotein LolB, at 1,081,051 to 1,081,668
_0946
Psyr_0947: Psyr_0947 - TPR repeat protein:TPR repeat protein, at 1,081,673 to 1,083,343
_0947
Position (kb) 1080
1081
1082 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1080.082 kb on - strand at 1081.796 kb on + strand at 1081.797 kb on - strand at 1081.858 kb on - strand, within Psyr_0947 at 1081.976 kb on - strand, within Psyr_0947 at 1081.976 kb on - strand, within Psyr_0947 at 1081.976 kb on - strand, within Psyr_0947 at 1081.976 kb on - strand, within Psyr_0947 at 1081.976 kb on - strand, within Psyr_0947 at 1081.978 kb on - strand, within Psyr_0947 at 1081.978 kb on - strand, within Psyr_0947
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 1,080,082 - -3.0 1,081,796 + -0.2 1,081,797 - +0.8 1,081,858 - Psyr_0947 0.11 -1.6 1,081,976 - Psyr_0947 0.18 -0.1 1,081,976 - Psyr_0947 0.18 -0.5 1,081,976 - Psyr_0947 0.18 -0.6 1,081,976 - Psyr_0947 0.18 -0.7 1,081,976 - Psyr_0947 0.18 -1.4 1,081,978 - Psyr_0947 0.18 -0.3 1,081,978 - Psyr_0947 0.18 -0.0
Or see this region's nucleotide sequence