Experiment: KB with Aristolochic Acid 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0700 and Psyr_0701 are separated by 240 nucleotides Psyr_0701 and Psyr_0702 are separated by 32 nucleotides Psyr_0702 and Psyr_0703 are separated by 216 nucleotides
Psyr_0700: Psyr_0700 - Trans-hexaprenyltranstransferase, at 803,577 to 804,545
_0700
Psyr_0701: Psyr_0701 - LSU ribosomal protein L21P, at 804,786 to 805,097
_0701
Psyr_0702: Psyr_0702 - LSU ribosomal protein L27P, at 805,130 to 805,405
_0702
Psyr_0703: Psyr_0703 - GTP-binding protein, HSR1-related:GTP1/OBG subdomain protein, at 805,622 to 806,845
_0703
Position (kb) 804
805
806 Strain fitness (log2 ratio) -1
0
1 at 804.583 kb on - strand at 804.583 kb on - strand at 804.583 kb on - strand at 804.583 kb on - strand at 804.609 kb on + strand at 804.610 kb on - strand at 804.610 kb on - strand at 804.688 kb on + strand at 805.455 kb on + strand at 805.461 kb on + strand at 805.573 kb on + strand at 805.573 kb on + strand at 805.573 kb on + strand at 805.573 kb on + strand at 805.573 kb on + strand at 805.577 kb on + strand at 805.577 kb on + strand at 805.577 kb on + strand at 805.577 kb on + strand at 805.577 kb on + strand at 805.582 kb on + strand at 805.582 kb on + strand at 805.582 kb on + strand at 805.582 kb on + strand at 805.582 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Aristolochic Acid 10 mM remove 804,583 - +0.1 804,583 - -1.3 804,583 - -1.2 804,583 - +0.4 804,609 + +0.3 804,610 - -0.3 804,610 - +0.0 804,688 + -0.0 805,455 + +0.0 805,461 + -0.2 805,573 + +0.5 805,573 + -1.0 805,573 + +0.0 805,573 + +0.4 805,573 + +0.4 805,577 + +0.6 805,577 + -1.1 805,577 + +0.3 805,577 + -1.1 805,577 + -0.8 805,582 + -1.5 805,582 + +0.0 805,582 + +0.1 805,582 + -0.5 805,582 + -0.1
Or see this region's nucleotide sequence